Purification and characterization of human T-lymphocyte-derived erythroid-potentiating activity.

Westbrook, Carol A.; Gasson, Judith C.; Gerber, Silke; Selsted, Michael E.; Golde, David W.

doi:10.1016/s0021-9258(18)90916-6

Cited by 80 publications

(2 citation statements)

References 29 publications

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“…TIMP-1 was once known as erythroid-potentiating activity (EPA) for inducing erythroid progenitor cells to grow and proliferate [22]. From these results, other studies aimed to investigate whether TIMP-1 induced cell growth in other cell lines.…”

Section: Cell Growthmentioning

confidence: 99%

Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer

Justo

Jasiulionis

2021

IJMS

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Tissue Inhibitor of Metalloproteases 1, also known as TIMP-1, is named for its well-established function of inhibiting the proteolytic activity of matrix metalloproteases. Given this function, many studies were carried out to verify if TIMP-1 was able to interrupt processes such as tumor cell invasion and metastasis. In contrast, many studies have shown that TIMP-1 expression is increased in several types of tumors, and this increase was correlated with a poor prognosis and lower survival in cancer patients. Later, it was shown that TIMP-1 is also able to modulate cell behavior through the induction of signaling pathways involved in cell growth, proliferation, and survival. The mechanisms involved in the regulation of the pleiotropic functions of TIMP-1 are still poorly understood. Thus, this review aimed to present literature data that show its ability to form a membrane complex with CD63 and β1-integrin, and point to N-glycosylation as a potential regulatory mechanism of the functions exerted by TIMP-1. This article reviewed the characteristics and functions performed individually by TIMP1, CD63, and β1-integrin, the roles of the TIMP-1/CD63/β1-integrin complex, both in a physiological context and in cancer, and the regulatory mechanisms involved in its assembly.

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Section: Cell Growthmentioning

confidence: 99%

Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer

Justo

Jasiulionis

2021

IJMS

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“…TIMP-1 promotes cell growth in fibroblasts, erythroid precursors and other cell types. TIMP-1 was first described as EPA, (19) because the stimulation of blood cell progenitors through TIMP-1 leads to an increased number of erythrocytes in vitro [327,119]. Later, it was discovered that EPA is identical to TIMP-1 [71] and it was shown that the effect of EPA on HSPCs (20) was independent of metallo- (16) Theses experiments were performed in collaboration with Matthias Wilk; University Hospital D üsseldorf; Clinic for Hematology, Oncology and Clinical Immunology; D üsseldorf; Germany (17) Tissue Inhibitor of Metalloproteinases (18) Programmed cell death (19) Erythroid Potentiating Activity (20) Human Hematopoietic Stem and Progenitor Cells proteinase activity [48].…”

Section: Timp-1 Signalingmentioning

confidence: 99%

Fast STED Microscopy

Lauterbach¹

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The spatial resolution of optical far-field microscopes was hampered by diffraction until STED microscopy broke the diffraction limit. This thesis for the first time combines the high spatial resolution of STED microscopy with high temporal resolution to Fast STED microscopy. It heralds the study of fast dynamic processes via STED microscopy: diffraction unlimited movies with 200 images per second are recorded from colloids; video-rate imaging is achieved in living cells. In particular, the diffusion of 36 nm particles is shown at 80 frames per second (fps); the processes at the growth front of a colloidal crystal of particles as small as 200 nm are visualized at 200 fps. Biological samples are imaged and analyzed beyond the proof of principle, driven by open questions from actual biological research: it is shown that the movement of neurotransmitter vesicles encompasses low and high mobility states, and that young and mature vesicles show a distinct motion behavior. The use of pulsed and continuous wave lasers is compared for Fast STED microscopy of living neurons. Customized solutions for the quantitative analysis of the movies, especially for the localization and tracking of neurotransmitter vesicles, are developed and tested in Monte Carlo simulations. The algorithms are extended to show the correlation of the metabolic rate of cells with the density of the Translocase of the Outer Membrane in mitochondria. Beam-scanning STED microscopy is extended to two-color imaging and protein interactions in human stem cells are therewith analyzed, which introduces high-resolution imaging into medical research. The co-localization of the proteins CD63, TIMP-1 and β1-Integrin is shown. The thesis also presents the historic evolution of the knowledge about the diffraction limit and discusses the influence of noise and pixilation on resolution. STED-Mikroskopie wird auf Zweifarbenaufnahmen ausgedehnt. Damit werden Proteininteraktionen in menschlichen Stammzellen untersucht; dies f ührt die optische hochauflösende Bildgebung auch in die medizinische Forschung ein. Die Kolokalisierung der Proteine CD63, TIMP-1 und β1-Integrin wird nachgewiesen. Diese Doktorarbeit enth ält auch einen historischen Abriß der Erkenntnisse zur Beugungsbegrenzung und diskutiert den Einfluß von Rauschen und Pixeln auf die Auflösung.

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Regulation of Erythropoietin Production

Fisher

1992

Comprehensive Physiology

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The sections in this article are: Physicochemical Properties of Erythropoietin Assay and Standardization of Erythropoietin Model for Kidney Production of Erythropoietin Renal Site of Production of Erythropoietin Extrarenal Erythropoietin Production Sites of Action of Erythropoietin Anemia of Renal Insufficiency Erythropoietin and Polycythemia Pharmacological Agents that Influence Erythropoietin Production Pharmacokinetics of Erythropoietin Potential Therapeutic uses of Erythropoietin Summary

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Purification and characterization of human T-lymphocyte-derived erythroid-potentiating activity.

Cited by 80 publications

References 29 publications

Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer

Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer

Fast STED Microscopy

Regulation of Erythropoietin Production

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