Purification and Characterization ofα-Glucuronidase from Snail Acetone Powder

A Chromogenic Substrate for a β-Xylosidase-Coupled Assay of α-Glucuronidase

“…There have been several successful attempts to synthesize 4-nitrophenyl ␣-D-glucuronide as a potential chromogenic substrate of ␣-glucuronidase (7,25,26). However, none of the tested microbial ␣-glucuronidases was found to be capable of hydrolyzing this substrate.…”

“…However, none of the tested microbial ␣-glucuronidases was found to be capable of hydrolyzing this substrate. The only exception was an ␣-glucuronidase isolated from the digestive tract of the snail Helix pomatia (7,26). Microbial enzymes apparently require the uronic acid to be linked to at least one xylopyranosyl residue.…”

Purification and Characterization of a Novel α-Glucuronidase fromAspergillus nigerSpecific forO-α-D-Glucosyluronic Acid α-D-Glucosiduronic Acid

“…4)-xylose (Table 3). 11,19,20) The V max value toward TreDC was reported to be only a few percent of that toward PNP -glucuronide, suggesting that TreDC is a poor substrate for the enzyme.…”

“…1,2,[6][7][8] It is known that -glucuronidases from bacteria and snail are composed of two subunits (Table 2). [3][4][5]9,11) Effects of pH, temperature, and chemicals As shown in Fig. 2, the enzyme was stable in a pH range of 2.0-4.0 at 30 C, and the optimum pH was pH 3.0-3.5.…”

Regulation of the α-glucuronidase-encoding gene (aguA) from Aspergillus niger

“…a-Glucuronidases have been purified from bacteria (Shao et al 1995;Ruile et al 1997), fungi (Uchida et al 1992;Siika-aho et al 1994;de Vries et al 1998) and from the snail Helix pomatia (Kawabata et al 1995). a-Glucuronidases are mainly active against short xylooligomers, but some activity has been detected against the polymeric substrate (de Vries et al 1998).…”