Purification and characterization of intracellular lipase from the polyunsaturated fatty acid-producing fungus Mortierella alliacea

Immobilization Effects on the Catalytic Properties of Two Fusarium Verticillioides Lipases: Stability, Hydrolysis, Transesterification and Enantioselectivity Improvement

“…In contrast, Ca 2+ activated Lip2 in about 45% in both concentrations. Calcium activation corroborates with literature results, since it is well-known that some lipases described are positively influenced by this ion, for the reason that in a calcium-free system, lipases are not able to adsorb at the water-fat interface and no lipolytic activity occurs [22,25,26]. On the other hand, it is known that heavy metals inhibit lipase activity as copper, aluminum and zinc ions.…”

“…Consequently, in this study Co 2+, Al 3+ and Cu 2+ ions and EDTA decreased Lip1 activity around 40% and Pb + and Zn 2+ decreased approximately 60% Lip2 activity, while other ions did not affect them significantly. The inhibition by Zn 2+ and Hg 2+ ions was observed for the lipase from Mortierela alliacea [26]. Generally, free lipases from mesophilic fungi are active and stable at temperatures between 25 and 40 • C [23].…”

Biodegradable Redox-Sensitive Star Polymer Nanomicelles for Enhancing Doxorubicin Delivery

“…In the presence of 10 mM DTT (Figure 4c,e), the particle size distribution after 8 hours was more uneven than the initial distribution (Figure 4a,d). Because the cracked disulfide bond of the polymer caused the hydrophilic shell to be broken and the hydrophobic fragments to aggregate in the PBS containing 10 mM DTT, when the micelles structure and the disulfide bond were destroyed, the heterochain polymer with many ester bonds could be hydrolyzed in the aqueous solution due to the polarity of the main chain, which was also the reason for the emergence of small molecules [34]. The redox sensitivity of the synthesized polymer was confirmed by DLS tests (Figure 4).…”

“…To investigate the in vitro stability of polymer 3 micelles and DOX-loaded polymer 3 micelles under reducing conditions, they were separately incubated in PBS containing 10 mM DTT with continuous oscillation for 8 h. In the presence of 10 mM DTT (Figure 4c,e), the particle size distribution after 8 h was more uneven than the initial distribution (Figure 4a,d). Because the cracked disulfide bond of the polymer caused the hydrophilic shell to be broken and the hydrophobic fragments to aggregate in the PBS containing 10 mM DTT, when the micelles structure and the disulfide bond were destroyed, the heterochain polymer with many ester bonds could be hydrolyzed in the aqueous solution due to the polarity of the main chain, which was also the reason for the emergence of small molecules [34]. The TEM images of polymer 3 micelles and drug-loaded polymer 3 micelles, as presented in Figure 5a,b, showed two core-shell spheres with the diameters of less than 170 nm.…”

Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

“…Colorimetric methods are widely used as reference method for determination of the specificity and the kinetic parameters of lipases. Several authors reported the assay of microbial lipase by colorimetric copper soap method [10,11,12]. Industries are still seeking strains of bacteria that produce a high yield of potent lipase with…”