Purification and Characterization of Lipase from thermophilic Geobacillus sp.

Mehta, Akshita; Saun, Nitin Kumar; Gupta, Reena

doi:10.2174/2211550105666151222183051

Cited by 5 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the survey performed in this work is for identifying the protein responsible for extracellular activity in B13-1 strain on olive oil, molecular size and cellular localization were analyzed. One of the putative lipase class 3 gave a negative result for signal peptide in SignalP program (502433) and its size is larger (115 kDa) than expected for extracellular lipases, which ranks from molecular weights less than 20 to 65 kDa [ 73 , 74 ]. The other three class 3 lipases were predicted with signal peptide and as being extracellular, making them good candidates ( Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

Canseco-Pérez

Castillo-Avila

Chí-Manzanero

et al. 2018

Genes

View full text Add to dashboard Cite

A lipolytic screening with fungal strains isolated from lignocellulosic waste collected in banana plantation dumps was carried out. A Trichoderma harzianum strain (B13-1) showed good extracellular lipolytic activity (205 UmL−1). Subsequently, functional screening of the lipolytic activity on Rhodamine B enriched with olive oil as the only carbon source was performed. The successful growth of the strain allows us to suggest that a true lipase is responsible for the lipolytic activity in the B13-1 strain. In order to identify the gene(s) encoding the protein responsible for the lipolytic activity, in silico identification and characterization of triacylglycerol lipases from T. harzianum is reported for the first time. A survey in the genome of this fungus retrieved 50 lipases; however, bioinformatic analyses and putative functional descriptions in different databases allowed us to choose seven lipases as candidates. Suitability of the bioinformatic screening to select the candidates was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The gene codifying 526309 was expressed when the fungus grew in a medium with olive oil as carbon source. This protein shares homology with commercial lipases, making it a candidate for further applications. The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and bioinformatic survey carried out herein, support the premise that the strategy can be used in other microorganisms with sequenced genomes to search for true lipases, or other enzymes belonging to large protein families.

show abstract

Section: Resultsmentioning

confidence: 99%

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

Canseco-Pérez

Castillo-Avila

Chí-Manzanero

et al. 2018

Genes

View full text Add to dashboard Cite

show abstract

“…The elution buffer used was 0.02 M Tris HCl buffer having pH 9.5. All eluted fractions were assayed both for lipase activity as well as total protein A280 and the fractions showing highest lipase activity were pooled and assayed for protein content 12 .…”

Section: Puri Cation Of Enzymementioning

confidence: 99%

“…Recently, it has been found that combination of rice straw and Mary olive cakes could be used as suitable and low-cost substrate for lipase production 10 . Because of inherent stability of thermostable lipase enzyme, they find various applications in industries and biotechnological sectors such as additives in detergents and food industries, environmental bioremediations and in molecular biology 11,12 . The ester s of salicylic acid are useful in preservation of food, pain control and fever control.…”

mentioning

confidence: 99%

Immobilization of Lipase from <i>Geobacillus</i> sp. and Its Application in Synthesis of Methyl Salicylate

2017

Self Cite

View full text Add to dashboard Cite

“…Microbial enzymes are widely used in different industries and their applications are mentioned below in Table 2 [47,48].…”

Section: Applications Of Microbial Enzymesmentioning

confidence: 99%

“…Novel developments in biotechnology allowed researchers to conduct safe gene transfer that codes for specific trait from one organism to other. This also helps to improve the production of enzymes and also supply in adequate proportion [48].…”

Section: Importance Of Microbial Enzymes In Biotechnologymentioning

confidence: 99%

Fermentative Production of Microbial Enzymes and their Applications: Present status and future prospects

2017

J App Biol Biotech

View full text Add to dashboard Cite

Microbial enzymes are widely used in different industries mainly because of vast availability of sources. Microbial enzymes could be genetically modified and are considered as economical in comparison to plant and animal enzymes. Production of microbial enzymes by application of fermentation procedures involves microbial propagation to get desired product. The process of fermentation is classified based on specific parameters. Microbial enzymes exhibit wide variety of applications in different industries like food, wine, dairy, baking, milling, beverages, and cereals. There are different techniques employed to produce microbial enzymes using downstream processing methods that are aimed at enzyme purification and recovery. The improvement in concentration, purity and percentage of recovery of enzymes can be achieved based on standard principles which are microbial sources, improvement of strain and application of membrane augmented downstream processing method to improve specific activity of enzyme. The article reviews on principles include microbial sources, methods of strain improvement and modern techniques associated with improvement of enzyme activity and recovery process. The application of microbial enzymes in various industries and their importance in biotechnology is highlighted.

show abstract

Purification and Characterization of Lipase from thermophilic Geobacillus sp.

Cited by 5 publications

References 0 publications

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

Immobilization of Lipase from <i>Geobacillus</i> sp. and Its Application in Synthesis of Methyl Salicylate

Fermentative Production of Microbial Enzymes and their Applications: Present status and future prospects

Contact Info

Product

Resources

About