Purification and characterization of membrane-bound peroxidases from Metroxylon sagu

Onsa, Galila Hassan; Saari, Nazamid; Selamat, Jinap; Bakar, Jamilah

doi:10.1016/j.foodchem.2003.07.013

Cited by 111 publications

(78 citation statements)

References 41 publications

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“…The isoenzymes, mPOD I and mPOD II membrane-bound peroxidases (mPOD I and mPOD II), from Metroxylon sagu also had pH optima at 6.0 and 5.5, respectively [43] with guaiacol substrate. Similarly, peroxidase from peels of Citrus reticulata var.…”

Section: Effect Of Phmentioning

confidence: 99%

Characterization of Plant Peroxidases and Their Potential for Degradation of Dyes: a Review

Kalsoom

Bhatti

Asgher

2015

Appl Biochem Biotechnol

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Peroxidases are ubiquitously found in all vascular plants and are promising biocatalysts for oxidization of wide range of aromatic substrates including various industrial dyes. Peroxidases can catalyze degradation of chemical structure of aromatic dyes either by precipitation or by opening the aromatic ring structure. Both soluble and immobilized peroxidases have been successfully used in batches as well as in continuous processes for the treatment of aromatic dyes present in industrial effluents. Plant peroxidases are stable catalysts that retain their activities over a broad range of pH and temperatures. The performance of an enzyme for degradation process depends upon the structure of dyes and the operational parameters like concentration of enzyme, H2O2 and dye, incubation time, pH, and temperature. Recalcitrant dyes can also be mineralized by plant peroxidases in the presence of redox mediators. Thus, plant peroxidases are easily available, inexpensive, and ecofriendly biocatalysts for the treatment of wastewaters containing a wide spectrum of textile and non-textile synthetic dyes. This article reviews the recent developments in isolation and characterization of plant peroxidases and their applications for bioremediation of synthetic dyes.

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Section: Effect Of Phmentioning

confidence: 99%

Characterization of Plant Peroxidases and Their Potential for Degradation of Dyes: a Review

Kalsoom

Bhatti

Asgher

2015

Appl Biochem Biotechnol

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“…Berkaitan dengan reaksi kopling oksidatif, enzim peroksidase adalah salah satu biokatalis kelas oksidoreduktase yang dapat memfasilitasi penggabungan molekul fenolik menjadi bentuk dimer maupun polimer (Onsa, bin Saari, Selamat, & Bakar, 2004). Pada penelitian terdahulu, guaiakol dapat mengalami dimerisasi yang dikatalisis oleh enzim peroksidase dari Brassica oleracea var alboglabra (Anita, Widiyarti, & Abbas, 2014).…”

Section: Gambar 1 Reaksi Kopling Oksidatif Eugenol Menggunakan Perokunclassified

Kopling Oksidatif Eugenol Menggunakan Ekstrak Enzim Peroksidase dari Akar Tanaman Sawi Hijau serta Uji Bioaktivitasnya

Ardiansah

Cahyana

Suwarso

et al. 2018

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Peroxidase has been successfully extracted from root of mustard greens (Brassica juncea L. Czern). The root partially purified using ammonium sulfate. This enzyme was applied as biocatalyst to coupling reaction of eugenol. The product was analyzed optical properties and antioxidant activity. Structure elucidation of product was conducted by FTIR, UV-vis, and GC-MS. Protein content and specific activities of peroxidase were 0.747 mg/mL and 0.244 U/mg, respectively. Peroxidase was applied as biocatalyst for green oxidative coupling reaction of eugenol to produce dimeric form of eugenol, an orange crystal (84% yields). The optical property of dimeric eugenol was Ra-configuration. Furthermore, biological activity of the product as antioxidant has been evaluated resulting in IC50 values of 32.14 and 42.43 ppm for dimeric and monomeric eugenol, respectively.

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“…(González et al 2000), sagu (Cycas revoluta Thunb.) (Onsa et al 2004 (Martínez et al 2001;Civello et al 1995), se empleó un pH de extracción y medición de 6,5. La velocidad de centrifugación se estableció basándose en que la técnica de disrupción seleccionada no debe ser más vigorosa que la requerida para causar ruptura en las células, ya que puede causar la inactivación de las enzimas una vez liberadas (Roe 2001).…”

Section: Metodología De Extracción De La Enzimaunclassified

Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.)

2013

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RESUMENEl objetivo de esta investigación fue obtener un protocolo de análisis de la actividad enzimática de Peroxidasa (POD) en pulpa de tomate de árbol (Solanum betaceum Cav.). Se evaluaron variables en el proceso de extracción como: el porcentaje de pulpa (PP), de 20 a 50% y el tiempo de centrifugación (TC) de 10 a 30 minutos; mientras que en la medición se varió el volumen de extracto (VE), de 30 a 90 L. Se utilizó un diseño experimental de composición central 23 más puntos estrellas y puntos centrales con metodología de superficie de respuesta con dos repeticiones y un total de 32 unidades experimentales. Para la optimización, la mayor actividad enzimática se presentó empleando un PP de 60%, un TC de 34 min y un VE de 110 µl.Palabras clave: Enzimas, actividad peroxidasa, frutales, pulpa, protocolo. ABSTRACTThe aim of this research was to obtain an analysis protocol of the enzymatic activity of Peroxidase (POD) in tree tomato pulp (Solanum betaceum Cav.). Variables were evaluated in the extraction process as the percentage of pulp (PP), of 20 to 50% and the centrifugation time (CT) of 10 to 30 min, while in the measurement was varied extract volume (EV) of 30 to 90 L. It was used an experimental central composite design 23 more star points and center points with response surface methodology replicated twice for a total of 32 experimental units. For optimization, the highest enzyme activity is presented using a PP of 60%, a CT of 34 min and a VE of 110 µl.

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Purification and characterization of membrane-bound peroxidases from Metroxylon sagu

Cited by 111 publications

References 41 publications

Characterization of Plant Peroxidases and Their Potential for Degradation of Dyes: a Review

Characterization of Plant Peroxidases and Their Potential for Degradation of Dyes: a Review

Kopling Oksidatif Eugenol Menggunakan Ekstrak Enzim Peroksidase dari Akar Tanaman Sawi Hijau serta Uji Bioaktivitasnya

Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.)

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