Purification and Characterization of Mitochondrial F₁-ATPase fromLilium longiflorumBulbs

Tissue specificity of mitochondrial F0F1‐ATPase activity of Lilium longiflorum plant

“…uate the contribution of F,-ATPase to the tissue specificity, F,-ATPase was purified to homogeneity from bulb and pollen SMP (Table 2) [5]. However, we could not explain the more than 10 times difference in F,F,-ATPase activities of bulbs and pollen, although the activity of pollen F,-ATPase was twice as high as that of the bulb.…”

“…F,-ATPase was dissolved out from SMP suspended in 1 mM EDTA, 4 mM ATP, 1 mM PMSF and 20 mM TES-KOH, pH 7.5, using CH,Q instead of CHCl, and further purified to homogeneity at room temperature by the method described previously [5]. The enzyme was dissolved in 0.25 M sucrose, 1 mM ATP, 1 mM dithiothreitol, 10 mM Tris-HCI, pH 7.5, and stored at -80°C until use.…”

“…ATPase activity was assayed calorimetrically by measuring Pi liberated [5]. A reaction mixture (1 ml) contained 3 mM ATP, 3 mM MgCl,, 20 mM MOPS-Tris, pH 8.0, and enzyme solution.…”

Partial characterization of potato F0F1‐ATPase Γ‐subunit cDNA and its regulation during fungal infection and elicitor treatment

“…Mitochondria were disrupted by sonication (2×20 s) at a maximum output (23 kHz, 50 W). ATPase activity was assayed colorimetrically by measurement of inorganic phosphate (P i ) released during a 10‐min incubation period at 30°C using Fiske‐Subbarow reagent (Itoh et al 1993). The reaction mixture (1 ml) contained 3 m M ATP, 3 m M MgCl 2 , 20 m M MOPS‐Tris, pH 8.0, and enzyme solution (storage buffer).…”