Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells

Xia, Hengchuan; Li, Feng; Li, Zhen; Zhang, Zu Chuan

doi:10.1038/sj.cr.7290182

Cited by 64 publications

(39 citation statements)

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“…Moreover, other proteins from pumpkin seeds were reported to inhibit melanoma proliferation (44) . Xia et al (45) isolated a novel ribosome-inactivating protein (RIP) called moschatin from the mature seeds of pumpkin (C. moschata) and a novel immunotoxin moschatin-Ng76 was prepared successfully which efficiently inhibits the growth of targeted melanoma cells M21 with an IC 50 (50 % inhibitory concentration) of 0·04 nM, 1500 times lower than that of free moschatin. Recently, Hou et al (46) isolated a novel type 1 RIP designated cucurmosin from the sarcocarp of C. moschata that exhibits strong cytotoxicity to three cancer cell lines of both human and murine origin, besides rRNA N-glycosidase activity.…”

Section: Anti-carcinogenic Effectmentioning

confidence: 99%

Medicinal and biological potential of pumpkin: an updated review

et al. 2010

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The use of herbal remedies individually or in combination with standard medicines has been used in various medical treatises for the cure of different diseases. Pumpkin is one of the well-known edible plants and has substantial medicinal properties due to the presence of unique natural edible substances. It contains several phyto-constituents belonging to the categories of alkaloids, flavonoids, and palmitic, oleic and linoleic acids. Various important medicinal properties including anti-diabetic, antioxidant, anti-carcinogenic, anti-inflammatory and others have been well documented. The purpose of the present article is to discuss various medicinal and biological potentials of pumpkin that can impart further research developments with this plant for human health benefits.

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Section: Anti-carcinogenic Effectmentioning

confidence: 99%

Medicinal and biological potential of pumpkin: an updated review

et al. 2010

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“…The fascinating multimodal activities of RIPs have since long time generated interest towards developing antitumor drugs that selectively target tumor cells 10 . A stern resolve in this regard, particularly in the case of type 1 RIPs, are the immunotoxins [11][12][13][14][15] , RIPs conjugated to specific antibodies, which may present promising options for treating various diseases and interestingly against HIV-infected cells 16 . The action mechanism of RIPs have been extremely imperceptible and numerous accounts have been put forth to decipher the same.…”

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confidence: 99%

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

Mohamed

Veeranarayanan

Minegishi

et al. 2014

Sci Rep

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Curcin, a type 1 ribosome inactivating protein (RIP) is investigated here for its cellular competence on six mammalian cell lines. Cells exposed to curcin (100 mg/ml) for 72 h exhibited significant cellular metabolic arrest, with the cancer cell lines being more sensitive. The viability assessment of the cancer cells in a 3D cell culture based assay revealed highly restricted sprouting and proliferation with near to complete dead cell population. Prominent mitochondrial dysfunction, elevated reactive oxygen species levels, nuclear degeneration, structural/mechanical destabilization and suppression of defense mechanisms were imminent with the RIP treated cells. Expression levels of nuclear factor kB (NF-kB), cytoskeletal focal adhesion kinases (FAK) and vinculin were significantly diminished. Vital cellular organelles as nucleus, mitochondria and actin were severely incapacitated on RIP exposure resulting in multimodal apoptosis and necrosis. The ability of curcin to impart comprehensive shutdown of the cells, especially cancer cells, complemented with its hemocompatibility, opens up possibilities of utilizing this ribotoxin as a prospective therapeutic candidate against cancers of diverse origins.

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“…3) To our knowledge, most of the chemical studies on pumpkin seeds were carried out on lipids in the pumpkin seed oil, [4][5][6] and no report has been published concerning the water-soluble constituents. The nutritive value and potential medicinal importance prompted us to investigate its chemical constituents, which resulted in the isolation of five new phenolic glycosides, cucurbitosides A-E (1)(2)(3)(4)(5). This paper deals with the isolation and structural elucidation of the new constituents on the basis of spectroscopic analysis and chemical evidence.…”

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New Phenolic Glycosides from the Seeds of Cucurbita moschata

Koike

Liu

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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Pumpkin, the fruits of Cucurbita moschata (Cucurbitaceae), has been used as a popular vegetable in cooking since antiquity and the pumpkin seeds are utilized in several countries as snacks after salting and roasting. The pumpkin seeds are also an important Traditional Chinese Medicine in the treatment of cestodiasis, ascariasis, and schistosomiasis. 1)Pharmacological studies on the seeds have demonstrated hepatoprotective 2) and antitumor activities.3) To our knowledge, most of the chemical studies on pumpkin seeds were carried out on lipids in the pumpkin seed oil, [4][5][6] and no report has been published concerning the water-soluble constituents. The nutritive value and potential medicinal importance prompted us to investigate its chemical constituents, which resulted in the isolation of five new phenolic glycosides, cucurbitosides A-E (1-5). This paper deals with the isolation and structural elucidation of the new constituents on the basis of spectroscopic analysis and chemical evidence.The seeds of C. moschata purchased in Harbin, China, were extracted with methanol. The methanolic extract was partitioned between ethyl acetate and water. The water layer was passed through a Diaion HP-20 column, and washed with methanol. The methanolic eluate was evaporated and applied to an ODS column chromatography (CC). Further purification by repeated reverse-phase HPLC gave five new phenolic glycosides, which were termed as cucurbitosides A-E (1-5).Cucurbitoside A (1) was isolated as an amorphous powder. The molecular formula was established as C 26 H 32 O 12 by high-resolution (HR)-FAB-MS. On acid hydrolysis, 1 afforded D-glucose and D-apiose as component sugars, which was identified by GLC analysis of its trimethylsilyl thiazolidine derivative.7) The 1 H-and 13 C-NMR spectra of 1 (Table 1) also revealed the presence of glucopyranose and apiofuranose moieties. The b-anomeric configuration for the glucopyranose was determined from a large 3 J H1,H2 coupling constant value (7.5 Hz). The b-anomeric configuration for the apiofuranose was suggested by the chemical shift of its anomeric carbon (d 110.5).8) Besides the signals due to sugars, the 1 H-NMR spectrum of 1 also showed a set of proton signals due to 1,4-disubstituted aromatic ring at d 6.95 (2H, dd, Jϭ8.9, 1.9 Hz) and 6.91 (2H, d, Jϭ8.9 Hz), as well as a benzylic methylene at d 2.65 (2H, td, Jϭ7.1, 1.9 Hz) and a hydroxymethyl at d 3.62 (2H, t, Jϭ7.1 Hz), indicating the presence of a 2-(4-hydroxyphenyl)ethanol moiety. Furthermore, the signals attributable to a monosubstituted aromatic ring were observed at d 7.94 (2H, dd, Jϭ8.3, 1.4 Hz), 7.41 (2H, t, Jϭ8.3 Hz) and 7.58 (1H, tt, Jϭ8.3, 1.4 Hz), in combination with the signal for a carbonyl carbon at d 167.8 in the 13 C-NMR spectrum, suggesting a benzoyl moiety in 1. The connection of the aforementioned structural units was determined by the 1 H-detected heteronuclear multiple bond connectivity (HMBC) data (Fig. 1). The b-D-glucopyranose was found to be connected to 4-OH of the 2-(4-hydroxy)phenylethanol moiety by ob...

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Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells

Cited by 64 publications

References 20 publications

Medicinal and biological potential of pumpkin: an updated review

Medicinal and biological potential of pumpkin: an updated review

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

New Phenolic Glycosides from the Seeds of Cucurbita moschata

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