Purification and characterization of novel raw-starch-digesting and cold-adapted α-amylases from Eisenia foetida

Ueda, Mitsuhiro; Asano, Takeyoshi; Nakazawa, Masami; Miyatake, Kazutaka; Inouye, Kuniyo

doi:10.1016/j.cbpb.2008.02.003

Cited by 47 publications

(27 citation statements)

References 20 publications

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“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed a single band near 58 kDa. This molecular weight falls within the range of values (54 to 68 kDa) reported for purified raw-starchdigesting amylase from other sources (Ueda et al, 2008).…”

Section: Electrophoresissupporting

confidence: 85%

Isolation and characterization of a cold-active amylase from marine Wangia sp. C52

Liu¹,

Zhang²,

Dang³

et al. 2011

Afr. J. Microbiol. Res.

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Amylase activity was detected in the culture medium of marine Wangia sp. C52, which was isolated from the Southern Okinawa Trough deep-sea sediment. In the present study, a cold-active amylase was purified to homogeneity from the culture broth by ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the amyalse was 58 kDa, and its isoelectric point was close to 5.6. The optimal pH and temperature were 30°C and 6.0, respectively. In the presence of Ca 2+ and Co 2+ , the enzyme activity was stimulated while Cu 2+ , Hg 2+ , Mn 2+ , Zn 2+ , Fe 3+ , Al 3+ , EDTA, EGTA and SDS reduced the activity. K m and V max values of the purified enzyme for soluble starch were 2.08 ± 0.3 mg/ml and 1.26 ± 0.02 mg/ml/min, respectively. The final purified enzyme had -helix of 25%, -sheet of 26% and random coil of 49%, consistent with the theoretical values. This showed that the purified amylase folded with a reasonable secondary structure.

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Section: Electrophoresissupporting

confidence: 85%

Isolation and characterization of a cold-active amylase from marine Wangia sp. C52

Liu¹,

Zhang²,

Dang³

et al. 2011

Afr. J. Microbiol. Res.

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“…SDS gel electrophoresis analysis manifests the presence of a single band near 66 kDa. This molecular weight matches with the previous reports (54 to 68 kDa) regarding the molecular weight of purified amylase from other sources [39].…”

Section: Purification Of Amylase Of Bacillus Subtilis (Atcc 6633) Frosupporting

confidence: 92%

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Mallik¹

2015

J Bioprocess Biotech

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Alpha amylase production using microbial source and solid state fermentation has been conducted for past few years in search of thermostable enzyme. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on. In the present work, Bacillus subtilis (ATCC 6633) has been utilized for generation of alpha amylase followed by optimization of the fermentation media. Change in fermentation conditions like fermentation hour, temperature, inoculums size, nitrogen and sugar sources have pivotal role to enhance alpha amylase yield. The thermal, pH and detergent stability of partially purified alpha amylase have been tested and compared with purified porcine pancreatic amylase. The result is encouraging with approximate 80% retention of alpha amylase activity comparable to purified porcine pancreatic amylase in presence of drastic condition of temperature (60°C), pH (6-11) and detergents. This makes it apt for use in various industries like detergent, food and paper industries.

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“…As enzimas digestórias encontradas em minhocas são responsáveis pela decomposição e humificação da matéria orgânica (Prabha et al, 2007). Neste sentido, Ueda et al (2008) caracterizaram, em minhocas E. foetida, dois tipos de α-amilases com propriedades termoestáveis sugerindo que ambas as enzimas possuem funções complementares e com alta atividade hidrolítica sobre o amido. Os resultados encontrados neste trabalho revelam que a enzima α-amilase de E. foetida não foi significativamente afetada durante os diferentes períodos de exposição à IVM.…”

Section: Methodsunclassified

“…É um processo bioquímico que tem a participação combinada de micro-organismos e minhocas que atuam na fragmentação e na degradação da matéria orgânica (Prabha et al, 2007;Ueda et al, 2008;Aira & Domínguez, 2009) possibilitando o reaproveitamento do esterco bovino, um resíduo sólido produzido em elevada quantidade durante o sistema de pecuária intensiva (Garg et al, 2006). Entretanto, a presença de vários compostos e metabólitos oriundos de fármacos veterinários e que são eliminados nas fezes de animais tratados, incide num risco ecológico no uso dos estercos (Floate et al, 2005) podendo, inclusive, comprometer o processo de vermicompostagem.…”

Section: Introductionunclassified

Indução de ivermectina na hormese sobre Eisenia foetida durante a vermicompostagem de esterco bovino

Tuerlinckx

Morselli

Huber

2015

Rev. bras. eng. agríc. ambient.

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R E S U M OOs medicamentos veterinários são, em sua maioria, excretados nas fezes de animais tratados demonstrando que os fármacos da classe das avermectinas podem ter efeitos nocivos sobre os organismos não alvo, caso se utilizem das fezes. O objetivo deste estudo foi determinar os efeitos subletais da ivermectina sobre as minhocas Eisenia foetida expostas às concentrações de ivermectina a 0, 1, 5, 10, 50 e 100 mg kg -1 ; amostras foram tomadas nos dias 7, 14 e 28 de exposição para determinação da sobrevivência, crescimento, produção de casulos e atividades enzimáticas, além de estudadas as alterações químicas e a atividade microbiana do vermicomposto não se observando, no entanto, efeitos negativos da ivermectina quanto à mortalidade de minhocas; da mesma forma, as características químicas e a atividade microbiana do vermicomposto não foram afetadas porém os resultados demonstraram que a ivermectina induz, quando em baixas concentrações, a um aumento na atividade da fosfatase alcalina e no crescimento de E. foetida mas altas concentrações inibiram esses parâmetros, o que foi refletido numa curva em forma de U invertido, uma representação gráfica do efeito hormético.Induction of ivermectin in the hormesis on Eisenia foetida during the vermicomposting of cattle manure A B S T R A C TMost of the veterinary pharmaceuticals are excreted in the faeces of treated animals and it has been demonstrated that members of the avermectin group may have deleterious effects on non-target organisms utilising the faeces. The aim of this study is to determine the sublethal effects of ivermectin on the Eisenia foetida earthworms. Earthworms were exposed to ivermectin at 0, 1, 5, 10, 50 and 100 mg kg -1 concentrations; samples were taken at 7, 14, and 28 days exposure for determination of survival, growth rate, cocoon production and enzyme activities. Furthermore, the chemical changes and microbial activity of the vermicompost were also studied. There was no negative effects of ivermectin on mortality of earthworms. Likewise the chemical characteristics and microbial activity of vermicompost were not affected. However, the results showed that ivermectin at low concentrations induces an increase in the activity of alkaline phosphatase and growth of E. foetida, but high concentrations inhibited these endpoints, and this was reflected in an inverted U-shaped curve, a graphic representation of hormetic effect. Palavras-chave:efeito hormético fármacos veterinários minhocas toxicidade Key words: earthworms hormetic effect toxicity veterinary pharmaceuticals

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Purification and characterization of novel raw-starch-digesting and cold-adapted α-amylases from Eisenia foetida

Cited by 47 publications

References 20 publications

Isolation and characterization of a cold-active amylase from marine Wangia sp. C52

Isolation and characterization of a cold-active amylase from marine Wangia sp. C52

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Indução de ivermectina na hormese sobre Eisenia foetida durante a vermicompostagem de esterco bovino

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