Purification and characterization of pectinmethylesterase from Aspergillus repens isolated from cultivated soil

Arotupin,; D, J; Akinyosoye,; F, A; Onifade,; A, K

doi:10.5897/ajb2008.000-5046

Cited by 22 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They reported that the Km value was 1.96mg/ml and Vmax value was 1.42 U/ml in starch hydrolysis. The results of current work are also similar with the findings of Arotupin et al (2008). They reported that the activity of enzyme produced by Aspergillus repens increased with increase in substrate concentration reaching maximum at 4 mg/ml and the lineweaver-Burk plot for the hydrolysis of pectin indicated approximately 1.3 mg/ml.…”

Section: Determination Of Km and Vmax Of Enzyme Activity From Starch supporting

confidence: 90%

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Shams

Kabir²,

Sharmin³

et al. 2019

Malaysian Journal of Halal Research

View full text Add to dashboard Cite

The research was concerned with the enzymatic hydrolysis followed by alcoholic fermentation of corn flour. Commercial corn flour purchased from local market contained starch of 87.25%, moisture 11.86% and ash 0.70%. The kinetics of corn flour hydrolysis was performed at 50°C, 65°C and 80°C using commercial thermos table-∞-amylase and ∞-amyloglucosidase. During hydrolysis the residual starch content was decreased with increasing hydrolysis time. The line weaver- Burk plot for enzyme hydrolysis of corn starch for 2.5%, 5% and 10% flour solution showed a Km value of 2.46, 2.27 and 1.73 mg/ml and Vmax values 1.23, 1.21 and 0.96 U/ml for 50°C, 65°C and 80°C respectively. Thin layer chromatography (TLC) was used to detect the presence of dextrose in the hydrolyzates. The Rf (Retention factors) values showed that the starch was mainly composed of dextrose and depended on carrier solvent use for dextrose assessment. When 100% ethyl alcohol used as carrier solvent the Rf value was 0.72 and ethyl alcohol: ethyl acetate= 1:1 the Rf value was 0.65. Both corn flour solution and its enzymatic hydrolysates were fermented with commercial baker’s yeast. Both the cases starch content was decreased and alcohol content was increased during hydrolysis and alcohol production was 3 times more from hydrolysates. The results obtained here will be useful for further research in the production of other value added products like white vinegar from the produced alcohol from con flour.

show abstract

Section: Determination Of Km and Vmax Of Enzyme Activity From Starch supporting

confidence: 90%

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Shams

Kabir²,

Sharmin³

et al. 2019

Malaysian Journal of Halal Research

View full text Add to dashboard Cite

show abstract

“…However, findings show B. cereus, B. firmus I-10104, B. cereus and B. endophyticus and B. coagulans exhibited maximum pectinase production at 37°C [90][91][92]. Simran and Vijay attested that Bacillus subtilis isolated from different agro-industrial wastes were able to produce pectinase at a temperature of 35ºC and at pH 7.0 [93]. The temperature range of millet cobs during degradation fall short of these values.…”

Section: Discussionmentioning

confidence: 99%

Millet Cobs: A Source of Microbial Enzymes

Ao¹,

Dj²

2018

J Microb Biochem Technol

View full text Add to dashboard Cite

show abstract

“…The method [7] was used for DNA isolation. Dissolved generic DNA samples from each of the A. niger isolates P1 -P6 were stored at -200°C.…”

Section: Preparation Of Genomic Dnamentioning

confidence: 99%

Purification and Characterization of Cellulase from <i>Aspergillus niger</i> Causing Soft Rot of White Yam in Three Environments in Nigeria

A.Adegboyega¹,

Sani²,

Dangwel³

et al. 2019

IJAAS

View full text Add to dashboard Cite

Aspergillus niger is abundant in most tropical soils and invariably, on the surface of yam tubers while still attached to the plant and on the root hairs during harvesting or storage. Naturally, the peridermic surface of the tubers function to exclude pathogen but damage caused by accidental incision or cut surface during weeding, insect attack and harvesting provide avenue for the infection. Aspergillus niger isolated from diseased yam in three zones in Nigeria synthesized cellulase which caused soft rot of the yam within nine days of inoculation. Microscopic and molecular analyses revealed two isolates of A. niger, P 1 and P 2, from different environment produced cellulase enzymes in significantly different proportions. When protein extracts from the infection were subjected to molecular exclusion chromatography, three peaks of absorption (A, B and C) were produced with only the components of peak A showing cellulase activity. Further fractionation of the components of peak A produced two absorption peaks (Aa and Ab) with only component Aa showing Cellulase activity. A. niger isolates, P 1 and P 2 showed considerable differences in the intensity of cellulase production suggesting that multiple strains of A. niger in the soil of yam-growing environments synthesized cellulase as transcriptional products in different manner underscoring the effect of physico-chemical properties of the soil on infectivity and virulence of the organism during yam rot.

show abstract

Purification and characterization of pectinmethylesterase from Aspergillus repens isolated from cultivated soil

Cited by 22 publications

References 13 publications

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Millet Cobs: A Source of Microbial Enzymes

Purification and Characterization of Cellulase from <i>Aspergillus niger</i> Causing Soft Rot of White Yam in Three Environments in Nigeria

Contact Info

Product

Resources

About

Purification and characterization of pectinmethylesterase from Aspergillus repens isolated from cultivated soil

Cited by 22 publications

References 13 publications

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Evaluation of enzymatic hydrolysis via alcoholic fermentation of corn flour

Millet Cobs: A Source of Microbial Enzymes

Purification and Characterization of Cellulase from &lt;i&gt;Aspergillus niger&lt;/i&gt; Causing Soft Rot of White Yam in Three Environments in Nigeria

Contact Info

Product

Resources

About

Purification and Characterization of Cellulase from <i>Aspergillus niger</i> Causing Soft Rot of White Yam in Three Environments in Nigeria