Purification and characterization of riproximin from Ximenia americana fruit kernels

Bayer, Helene; Ey, Noreen; Wattenberg, Andreas; Voss, Cristina; Berger, Martin R.

doi:10.1016/j.pep.2011.11.018

Cited by 23 publications

(9 citation statements)

References 31 publications

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“…25 For the in vitro and in vivo experiments, Rpx was diluted in full medium or in sterile physiological saline containing 5% albumin, respectively. Animals All animal experiments were performed in accordance with the Regierungspräsidium Karlsruhe, which as Institutional Animal Care and Use Committee (IACUC) approved the animal ethics of this study for the German Cancer Research Center (DKFZ).…”

Section: Cell Culturementioning

confidence: 99%

Riproximin’s activity depends on gene expression and sensitizes PDAC cells to TRAIL

Adwan

Murtaja

Al-Taee

et al. 2014

Cancer Biology & Therapy

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Section: Cell Culturementioning

confidence: 99%

Riproximin’s activity depends on gene expression and sensitizes PDAC cells to TRAIL

Adwan

Murtaja

Al-Taee

et al. 2014

Cancer Biology & Therapy

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“…However, this cytotoxicity of Rpx against different cancer cell lines varied over a broad range (maximum by a factor of 100), which is possibly due to differential expression of receptors required for Rpx binding, specific molecular routes and nega-tive feedback mechanisms developed by the cancer cells over time. Nevertheless, Rpx illustrated specific toxicity towards tumor cell lines, whereas non-tumor cell lines showed either no or only a marginal sensitivity (16)(17)(18)(19). Further investigations on the antineoplastic potential of Rpx were accomplished in colorectal and pancreatic cancer liver metastasis rat animal models.…”

Section: Introductionmentioning

confidence: 99%

Riproximin: A type II ribosome inactivating protein with anti-neoplastic potential induces IL24/MDA-7 and GADD genes in colorectal cancer cell lines

Pervaiz

Adwan

Berger

2015

International Journal of Oncology

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Riproximin (Rpx) is a type II ribosome inactivating protein, which was extracted and purified from the seeds of Ximenia americana. Previous studies demonstrated cytotoxicity of Rpx against a variety of cell lines originating from solid and non-solid cancers. In this study, we investigated the mechanistic aspects of Rpx in selected human and rat colorectal cancer (CRC) cell lines. Cytotoxic levels of Rpx were determined by MTT assay, while cytostatic and apoptotic effects were investigated by flow cytometry and nuclear staining procedures. Effects of Rpx exposure on colony formation/migration of CRC cells and expressional modulations in anticancer/stress-related genes were also studied. Rpx showed significant and comparable levels of cytotoxicity in CRC cells as determined by inhibitory concentration (IC) values. Similar inhibitory effects were found for clonogenicity, while more pronounced inhibition of migration was observed in response to Rpx exposure. Profound arrest in S phases of the cell cycle was noted especially in primary CRC cells. Apoptotic effects were more prominent in rat CRC cells as indicated by Annexin V-FITC assay and Hoechst 33342 nuclear staining. Rpx exposure induced significantly increased levels of the IL24/MDA-7, a well characterized anticancer gene, in all CRC cells. In addition, following Rpx treatment, high expression levels of growth arrest and DNA damage (GADD family) genes were also observed. Increased expression of two additional GADD genes (34 and 153) only in rat CRC cells (CC531) conferred higher sensitivity towards Rpx and subsequent anti-proliferative/apoptotic effects as compared to human CRC cells (SW480 and SW620). The present investigation indicates the anticancer potential of Rpx in CRC and favor further evaluation of this natural compound as therapeutic agent.

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“…In this follow-up study, we investigated commonalities between Rpx and LSBPs and assessed the effect of Rpx on tumor derived LSBPs with regard to metastasis. The rationale of the study based on the same method, which had been used for isolating these proteins (Bayer et al, 2012b;Sagini et al, 2020). Voss et al showed that Rpx binds to galactose, a feature that led to its purification by affinity chromatography (Voss et al, 2006b).…”

Section: Discussionmentioning

confidence: 99%

“…Besides inhibiting protein synthesis, Rpx was also shown to induce cell death through unfolded protein response (Horrix et al, 2011). Rpx can be purified from the fruits of Ximenia americana by affinity chromatography using a resin coupled with lactosyl residues (Bayer et al, 2012b). When evaluated by sequence analysis and molecular modeling, Rpx was found to interact with galactose via its two sub-domains in the B-chain (Voss et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

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Riproximin Exhibits Diversity in Sugar Binding, and Modulates some Metastasis-Related Proteins with Lectin like Properties in Pancreatic Ductal Adenocarcinoma

et al. 2020

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Riproximin (Rpx) is a type II ribosome-inactivating protein with specific anti-proliferative activity. It was purified from Ximenia americana by affinity chromatography using a resin coupled with lactosyl residues. The same technique facilitated isolation of proteins with lectin-like properties from human Suit2-007 and rat ASML pancreatic cancer cells, which were termed lactosyl-sepharose binding proteins (LSBPs). The role of these proteins in cancer progression was investigated at mRNA level using chip array data of Suit2-007 and ASML cells re-isolated from nude rats. These data compared significant mRNA expression changes when relating primary (pancreas) and metastatic (liver) sites following orthotopic and intraportal implantation of Pancreatic Ductal Adenocarcinoma (PDAC) cells, respectively. The affinity of Rpx to 13 simple sugar structures was modeled by docking experiments, the ranking of which was principally confirmed by NMR-spectroscopy. In addition, Rpx and LSBPs were evaluated for anti-proliferative activity and their cellular uptake was assessed by fluorescence microscopy. From 13 monosaccharides evaluated, open-chain rhamnose, β-d-galactose, and α-l-galactopyranose showed the highest affinities for site 1 of Rpx’s B-chain. NMR evaluation yielded a similar ranking, as galactose was among the best binders. Both, Rpx and LSBPs reduced cell proliferation in vitro, but their anti-proliferative effects were decreased by 15–20% in the presence of galactose. The program “Ingenuity Pathway Analysis” identified 2,415 genes showing significantly modulated mRNA expression following exposure of Suit2-007 cells to Rpx in vitro. These genes were then matched to those 1,639 genes, which were significantly modulated in the rat model when comparing primary and metastatic growth of Suit2-007 cells. In this overlap analysis, LSBP genes were considered separately. The potential suitability of Rpx for treating metastatic Suit2-007 PDAC cells was reflected by those genes, which were modulated by Rpx in a way opposite to that observed in cancer progression. Remarkably, these were 14% of all genes modulated during cancer progression, but 71% of the respective LSBP gene subgroup. Based on these findings, we predict that Rpx has the potential to treat PDAC metastasis by modulating genes involved in metastatic progression, especially by targeting LSBPs.

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Purification and characterization of riproximin from Ximenia americana fruit kernels

Cited by 23 publications

References 31 publications

Riproximin’s activity depends on gene expression and sensitizes PDAC cells to TRAIL

Riproximin’s activity depends on gene expression and sensitizes PDAC cells to TRAIL

Riproximin: A type II ribosome inactivating protein with anti-neoplastic potential induces IL24/MDA-7 and GADD genes in colorectal cancer cell lines

Riproximin Exhibits Diversity in Sugar Binding, and Modulates some Metastasis-Related Proteins with Lectin like Properties in Pancreatic Ductal Adenocarcinoma

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