Purification and characterization of silkworm hemocytes by flow cytometry

Nakahara, Yuko; Shimura, Sachiko; Ueno, Chihiro; Kanamori, Yasushi; Mita, Kazuei; Kiuchi, Makoto; Kamimura, Manabu

doi:10.1016/j.dci.2008.09.005

Cited by 61 publications

(75 citation statements)

References 46 publications

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“…The biological role of spherulocytes in insects has not been elucidated to date (Strand, 2008). Nakahara et al (2009) performed a transcriptome analysis of each hemocyte subset and revealed that several insect immune-related genes expressed in other hemocyte subsets were rarely detected in spherulocytes, suggesting that spherulocytes are not involved in the immune system, and thus likely to be distinct from those of the other subsets. It has been known that several strains of B. mori lack this hemocyte subset (Nittono, 1960).…”

Section: Resultsmentioning

confidence: 98%

Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

Hori

Kiuchi

Shimada

et al. 2013

Journal of Invertebrate Pathology

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Section: Resultsmentioning

confidence: 98%

Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

Hori

Kiuchi

Shimada

et al. 2013

Journal of Invertebrate Pathology

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“…But there are five types of hemocyte in B. mori, including prohemocyte, oenocytoid, plasmatocyte, granulocyte and spherulocyte (Nakahara et al, 2009;Tan et al, 2013). Oenocytoid in B. mori is similar to the crystal cell in D. melanogaster (Strand, 2008).…”

Section: Discussionmentioning

confidence: 97%

A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph

Wang

Tan

et al. 2015

Developmental & Comparative Immunology

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“…Because of their body size, a large number of hemocytes can be obtained from silkworms for analysis of the functions of each subtype. Nakahara et al (41) established a method for purifying different subtypes of hemocytes in B. mori. These technical advantages of silkworms offer the prospect of identifying the specific hemocyte subtype responsible for promoting phagocytosis induced by active PP.…”

Section: Discussionmentioning

confidence: 99%

Insect Cytokine Paralytic Peptide (PP) Induces Cellular and Humoral Immune Responses in the Silkworm Bombyx mori

Ishii

Hamamoto

Kamimura

et al. 2010

Journal of Biological Chemistry

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In the blood (hemolymph) of the silkworm Bombyx mori, the insect cytokine paralytic peptide (PP) is converted from an inactive precursor to an active form in response to the cell wall components of microorganisms and contributes to silkworm resistance to infection. To investigate the molecular mechanism underlying the up-regulation of host resistance induced by PP, we performed an oligonucleotide microarray analysis on RNA of blood cells (hemocytes) and fat body tissues of silkworm larvae injected with active PP. Expression levels of a large number of immune-related genes increased rapidly within 3 h after injecting active PP, including phagocytosis-related genes such as tetraspanin E, actin A1, and ced-6 in hemocytes, and antimicrobial peptide genes cecropin A and moricin in the fat body. Active PP promoted in vitro and in vivo phagocytosis of Staphyloccocus aureus by the hemocytes. Moreover, active PP induced in vivo phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) in the fat body. Pretreatment of silkworm larvae with ML3403, a pharmacologic p38 MAPK inhibitor, suppressed the PP-dependent induction of cecropin A and moricin genes in the fat body. Injection of active PP delayed the killing of silkworm larvae by S. aureus, whereas its effect was abolished by preinjection of the p38 MAPK inhibitor, suggesting that p38 MAPK activation is required for PP-dependent defensive responses. These findings suggest that PP acts on multiple tissues in silkworm larvae and acutely activates cellular and humoral immune responses, leading to host protection against infection.

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Purification and characterization of silkworm hemocytes by flow cytometry

Cited by 61 publications

References 46 publications

Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph

Insect Cytokine Paralytic Peptide (PP) Induces Cellular and Humoral Immune Responses in the Silkworm Bombyx mori

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