2022
DOI: 10.1128/aem.00029-22
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Purification and Characterization of the Isoprene Monooxygenase from Rhodococcus sp. Strain AD45

Abstract: Isoprene is a highly abundant climate-active gas and a carbon source for some bacteria. Analyses of the genes encoding isoprene monooxygenase (IsoMO) indicate this enzyme is a soluble diiron center monooxygenase in the same family of oxygenases as soluble methane monooxygenase, alkene monooxygenase, and toluene monooxygenase.

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Cited by 6 publications
(14 citation statements)
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“…AD45 (Crombie et al, 2015 ; Dawson et al, 2020 ; Gibson et al, 2020 ; Larke‐Mejía et al, 2020 ; van Hylckama Vlieg et al, 2000 ). Isoprene monooxygenase (IsoMO, encoded by isoABCDEF ) catalyses the initial oxidation of isoprene to epoxyisoprene (Crombie et al, 2015 ; Dawson et al, 2020 ; Sims et al, 2022 ; van Hylckama Vlieg et al, 2000 ). An isoprene pathway‐specific glutathione S ‐transferase, IsoI, then conjugates epoxyisoprene with glutathione, a thiol not typically found in Gram‐positive bacteria (Johnson et al, 2009 ), which was also implicated in the mechanistically similar styrene metabolic pathway of Gordonia rubripertincta CWB2 (Heine et al, 2018 ), forming 1‐hydroxy‐2‐glutathionyl‐2‐methyl‐3‐butene (HGMB) (van Hylckama Vlieg et al, 1999 ).…”
Section: Introductionmentioning
confidence: 99%
“…AD45 (Crombie et al, 2015 ; Dawson et al, 2020 ; Gibson et al, 2020 ; Larke‐Mejía et al, 2020 ; van Hylckama Vlieg et al, 2000 ). Isoprene monooxygenase (IsoMO, encoded by isoABCDEF ) catalyses the initial oxidation of isoprene to epoxyisoprene (Crombie et al, 2015 ; Dawson et al, 2020 ; Sims et al, 2022 ; van Hylckama Vlieg et al, 2000 ). An isoprene pathway‐specific glutathione S ‐transferase, IsoI, then conjugates epoxyisoprene with glutathione, a thiol not typically found in Gram‐positive bacteria (Johnson et al, 2009 ), which was also implicated in the mechanistically similar styrene metabolic pathway of Gordonia rubripertincta CWB2 (Heine et al, 2018 ), forming 1‐hydroxy‐2‐glutathionyl‐2‐methyl‐3‐butene (HGMB) (van Hylckama Vlieg et al, 1999 ).…”
Section: Introductionmentioning
confidence: 99%
“…The biochemistry of isoprene metabolism is an emerging area of research. Recently, Sims et al (2022) described the expression and purification of the isoprene monooxygenase from Rhodococcus sp. AD45 in homologous and heterologous hosts, paving the way for detailed biochemical characterizations and further study of the ability of this enzyme to produce valuable chiral epoxides.…”
Section: Future Perspectivesmentioning
confidence: 99%
“…Iso proteins have been purified from Rhodococcus sp. AD45 (Sims et al, 2022), permitting in‐depth biochemical analyses. Evidence of a β‐oxidative metabolic pathway for the assimilation of carbon from isoprene via propionyl‐CoA, initially predicted by van Hylckama Vlieg et al (2000), has also been reported (Dawson et al, 2022), leading to the prediction of the complete pathway by which isoprene‐derived carbon is assimilated.…”
Section: Introductionmentioning
confidence: 99%
“…All isoprene degraders examined contain six genes ( isoABCDEF ) encoding an α 2 β 2 γ 2 oxygenase (IsoABE), a reductase (IsoF), a Rieske-type ferredoxin (IsoC), and a coupling protein (IsoD) that form the isoprene monooxygenase (IsoMO) ( 24 ). Five additional genes ( isoGHIJ and aldH ), encoding a putative coenzyme A transferase, an NAD + -dependent dehydrogenase, two glutathione S -transferases (GSTs), and a putative dehydrogenase (AldH), respectively, are located upstream of the IsoMO-encoding genes ( 22 , 25 ).…”
Section: Introductionmentioning
confidence: 99%
“…AD45 were described by van Hylckama Vlieg et al ( 20 , 25 , 27 ). Briefly, isoprene is oxidized to epoxyisoprene by IsoMO ( 24 ). The epoxide is then detoxified by conjugation with glutathione, a low-molecular-mass thiol not typically found in Gram-positive bacteria ( 28 , 29 ), forming 1-hydroxy-2-glutathionyl-2-methyl-3-butene (HGMB), a reaction catalyzed by IsoI.…”
Section: Introductionmentioning
confidence: 99%