2005
DOI: 10.1007/s11120-005-5103-2
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Purification and Characterization of the B808–866 Light-harvesting Complex from Green Filamentous Bacterium Chloroflexus aurantiacus

Abstract: The integral membrane light-harvesting complex B808-866 from the thermophilic green filamentous bacterium Chloroflexus aurantiacus has been isolated and characterized. Reversed-phase HPLC analysis demonstrated that the number of bacteriochlorophyll (BChl) in the B808-866 antenna complex is 36 +/- 2 per reaction center. The main carotenoid type is gamma-carotene, and the molar ratio of BChl to carotenoid is 3:2. The steady-state absorption and fluorescence spectroscopy of the B808-866 complex are reminiscent of… Show more

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Cited by 27 publications
(20 citation statements)
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“…[15] Like in LH2, it contains two rings of BChl pigments (i.e., B808 and B866), however, the number of pigments in the rings is different. As has been suggested in [16], the B808 ring contained 24 and the B866 ring 48 BChl molecules.…”
Section: Structural Models For the Antennae And The Reaction Centersupporting
confidence: 54%
“…[15] Like in LH2, it contains two rings of BChl pigments (i.e., B808 and B866), however, the number of pigments in the rings is different. As has been suggested in [16], the B808 ring contained 24 and the B866 ring 48 BChl molecules.…”
Section: Structural Models For the Antennae And The Reaction Centersupporting
confidence: 54%
“…Both samples exhibited peaks at 465 and 745 nm that originated from aggregated BChl c within intact chlorosomes. A weak peak around 675 nm indicated a small amount of monomeric BChl c or bacteriopheophytin c. The spectrum of one sample included prominent features at 805 and 870 nm, which were assigned to residual amounts of B806-866 light-harvesting complexes (46). These complexes reside in the underlying bacterial membrane and are not part of the chlorosome.…”
Section: Resultsmentioning
confidence: 99%
“…Previous studies suggest that the building blocks of the LHB808-866 antenna from Cf. aurantiacus spectrally similar to LH2 complexes from purple bacteria, but topologically similar to LH1 complexes (Feick et al 1995;Novoderezhkin et al 1998;Xin et al 2005;Collins et al 2009Collins et al , 2010. The physiological significance of this mosaic antenna is yet to be revealed.…”
Section: Introductionmentioning
confidence: 90%