2004
DOI: 10.1007/bf02931639
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Purification and characterization of xylanases fromAspergillus giganteus

Abstract: A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for xylanase I and 6.0 for xylanase II. At 50 degrees C xylanase I exhibited higher thermostability than xylanase II. Hg2+, Cu2+ and SDS were strong inhibitors, 1,4-dithiothreitol stimulated the react… Show more

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Cited by 45 publications
(41 citation statements)
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“…Xylanases have been purified to homogeneity with a molecular mass of 32-35 kDa from A. awamori 2B.361 U2/1 12 11 . The purification factor for xylanases from Aspergillus strains was relatively low as 1.3-4 8,14 and also in this study, middle high: 30-33 10,15,20 or very high: 77-93 11 . The purification yield of the xylanase from A. awamori VTCC-F312 was the highest (22%) among purification yield reported; low as 3.4-4.5% (xylanase II 8 , xylanase I 11, 14 ), middle high as 10.5-14.8% (xylanase II 11 , xylanase I 8, 15 ), and high as 20.7% 9 .…”
Section: Purification Of a Awamori Vtcc-f312 Xylanasesupporting
confidence: 69%
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“…Xylanases have been purified to homogeneity with a molecular mass of 32-35 kDa from A. awamori 2B.361 U2/1 12 11 . The purification factor for xylanases from Aspergillus strains was relatively low as 1.3-4 8,14 and also in this study, middle high: 30-33 10,15,20 or very high: 77-93 11 . The purification yield of the xylanase from A. awamori VTCC-F312 was the highest (22%) among purification yield reported; low as 3.4-4.5% (xylanase II 8 , xylanase I 11, 14 ), middle high as 10.5-14.8% (xylanase II 11 , xylanase I 8, 15 ), and high as 20.7% 9 .…”
Section: Purification Of a Awamori Vtcc-f312 Xylanasesupporting
confidence: 69%
“…The purification factor for xylanases from Aspergillus strains was relatively low as 1.3-4 8,14 and also in this study, middle high: 30-33 10,15,20 or very high: 77-93 11 . The purification yield of the xylanase from A. awamori VTCC-F312 was the highest (22%) among purification yield reported; low as 3.4-4.5% (xylanase II 8 , xylanase I 11, 14 ), middle high as 10.5-14.8% (xylanase II 11 , xylanase I 8, 15 ), and high as 20.7% 9 . The specific activity of the xylanase in this study (217 U/mg) was higher than that (124 U/mg) of the A. niger C3486 xylanase 15 , relatively high as that (289 U/mg) of the xylanase from A. ficuum AF-98 U2/1 (490 U/mg) 12 and A. cf.…”
Section: Purification Of a Awamori Vtcc-f312 Xylanasesupporting
confidence: 69%
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