Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry

Ren, Jiaoyan; Zhao, Mouming; Shi, John; Wang, Jinshui; Jiang, Yueming; Cui, Chun; Kakùda, Yukio; Xue, Sophia Jun

doi:10.1016/j.foodchem.2007.11.010

Cited by 319 publications

(242 citation statements)

References 36 publications

Supporting

Mentioning

213

Contrasting

Unclassified

Order By: Relevance

“…However, as described above, FIII clearly showed the highest antioxidant activity measured by both FRAP and ABTS. In agreement with other studies, besides the amino acid profile and the molecular weight, the amino acid sequence of the peptides may greatly contribute to generate the antioxidant activity observed (Suetsuna, Ukeda, & Ochi, 2000;Ren et al, 2008).…”

Section: Antioxidant Activity and Amino Acid Composition Of Fractionssupporting

confidence: 91%

See 1 more Smart Citation

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

et al. 2011

View full text Add to dashboard Cite

** This centre has implemented and maintains a Quality Management System which fulfils the requirements of the ISO standard 9001:2000 AbstractSquid gelatin obtained from inner and outer tunics was hydrolyzed with Alcalase to isolate antioxidant peptide sequences. The ACE-inhibitory activity of the isolated peptides was also evaluated. After fractionation by ultrafiltration and size-exclusion chromatography into four fractions, the antioxidant activity of the peptide fractions was determined by radical scavenging ability and ferric reducing power. Fraction FIII showed the highest antioxidant activity, although slight differences could be expected in the antioxidant activity of the different fractions based on the amino acid composition.FIII was subjected to liquid chromatography and tandem mass spectrometry (LC-MS/MS) and two major compounds were identified: the compound with m/z 952.42, which could be mostly comprised by the carbohydrate fucose, and the peptide with m/z 1410.63. Three possible sequences were proposed for this peptide, and the contribution of Leu or Hyp residues to the antioxidant and ACE-inhibitory activities of the resulting sequence was evaluated. The presence of Leu residues in the peptide sequence in *Manuscript Click here to view linked References 2 replacement of Hyp seems to play an important role in the antioxidant and ACEinhibitory activity.

show abstract

Section: Antioxidant Activity and Amino Acid Composition Of Fractionssupporting

confidence: 91%

“…Therefore, the amino acid sequence of the peptide might play an important role in its activity (Ren et al, 2008;Suetsuna et al, 2000). Leu has been reported to enhance the scavenging activities of peptides Li et al, 2007), as it has been also shown in this study.…”

Section: Antioxidant Activity Of Synthetically Derived Peptidessupporting

confidence: 77%

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Recent studies have demonstrated that protein hydrolysates from fish source possess antioxidant activities after enzymatic hydrolysis using various proteolytic enzymes such as bromelain, papain, flavorzyme, protamex, neutrase, and alcalase (Lamsal et al 2007;Ren et al 2008;Tanuja et al 2012). Among these enzymes, bromelain hydrolysate from freshwater carp (Catla catla) is appearing to give high disappearance rates of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Elavarasan et al 2013).…”

Section: Protein Hydrolysatementioning

confidence: 99%

Bromelain: an overview of industrial application and purification strategies

Arshad

Amid

Yusof

et al. 2014

Appl Microbiol Biotechnol

168

134

View full text Add to dashboard Cite

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

show abstract

“…Peptides with higher ratios of hydrophobic amino acids (e.g. tyrosine, tryptophan, phenylalanine, histidine, methionine or cysteine) are considered more effective (Ren et al 2008). Aromatic residues, such as tryptophan (indolic group) or tyrosine (phenolic group), may stabilise ROS by means of direct electron transfer and resonance .…”

Section: Effect Of Oxidative Stressors On Cytotoxicity Of Hepg2 Cellmentioning

confidence: 99%

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

View full text Add to dashboard Cite

Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H 2 O 2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P>0.05). Cell reactive oxygen species (ROS) generation as mediated by H 2 O 2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H 2 O 2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.

show abstract

Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry

Cited by 319 publications

References 36 publications

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

Bromelain: an overview of industrial application and purification strategies

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Contact Info

Product

Resources

About