Purification and neuroprotective effects of polysaccharides from Opuntia Milpa Alta in cultured cortical neurons

Yang, Changzhu; Zhao, Bo; Huang, Xiaolan; Zhan, Jinjin; Zhao, Yan; Guo, Lan

doi:10.1016/j.ijbiomac.2011.06.031

Cited by 15 publications

(4 citation statements)

References 31 publications

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“…Generally, polysaccharides are assumed to have no physiological effect, thus being considered as an optimal supportive material for medical applications. Recently, some polysaccharides showed therapeutic potentials for the treatment of neurodegenerative diseases [4] , [5] , [6] , [7] , [8] , [9] . Some appear to elicit responses similarly to an endogenous polysaccharide, polysialic acid (PSA) that enhances neurogenesis in neurons [10] , [11] .…”

Section: Additional Informationmentioning

confidence: 99%

Use of fluorescent ANTS to examine the BBB-permeability of polysaccharide

et al. 2015

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Section: Additional Informationmentioning

confidence: 99%

Use of fluorescent ANTS to examine the BBB-permeability of polysaccharide

et al. 2015

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“…However, at present, there are few studies on lactase activity in vitro, especially the effect of polysaccharides on lactase activity is rarely reported. In previous research, it was found that the inhibition rate of MAP-2 on α-glucosidase was significantly higher than that of acarbose at the same concentration, and as an edible plant, Opuntia Milpa Alta is relatively safe [ 37 ]. At the same time, it also had a good activation effect on lactase.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Extraction Process of Polysaccharides MAP-2 from Opuntia Milpa Alta by Response Surface Methodology and Evaluation of Its Potential as α-Glucosidase Inhibitor

Yang

Zhou

et al. 2022

Foods

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The α-glucosidase inhibitors play an important role in blood glucose control in patients with type 2 diabetes. At present, the development of new α-glucosidase inhibitors is an urgent clinical need. Our previous studies have found that the polysaccharide MAP-2 in the cactus Opuntia Milpa Alta has significantly better activity than acarbose (one of the most widely used first-line α-glucosidase inhibitors in clinical practice), but its optimal extraction process parameters and inhibition kinetic characteristics are not clear, and whether it has the potential to become a new α-glucosidase inhibitors is also unclear. In this study, based on previous research, we used the combination of single factor experiments and the response surface method (RSM) to identify the optimal extraction conditions for MAP-2 as follows: solid-liquid ratio 1:4, extraction temperature 90 °C, extraction time 1 h. Under these conditions, the extraction yield of MAP-2 was 3.47 ± 0.062%. When the concentration of MAP-2 was 16 mg/mL, the inhibition rate of α-glucosidase was 91.13 ± 0.62%. In addition, the results of inhibition kinetics showed that the inhibition rate of MAP-2 on α-glucosidase was the highest at pH 7.4 for 30 min, and showed a good dose-effect relationship, which was a reversible competitive inhibition. Meanwhile, we also compared the activities of MAP-2 and acarbose on the side effects of acarbose related enzymes. Compared with acarbose, MAP-2 not only had a better activation effect on lactase, but also inhibited the activity of hyaluronidase, and the activation and inhibition rate were positively correlated with the concentration. However, under the same conditions, the effect of acarbose on hyaluronidase was opposite to that of MAP-2. At low concentration, acarbose had a certain activation effect on lactase, but gradually attained an inhibitory effect with the increase in concentration. In contrast, MAP-2 not only activates lactase activity, improves diarrhea, abdominal distension, and abdominal pain, but also inhibits hyaluronidase activity, to solve the side effects of allergic reactions, suggesting that MAP-2 has the potential to become a novel and effective inhibitor of α-glucosidase with fewer side effects.

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“…Also, sulfate groups of sulfated PS trapped free radicals electrostatically 42 . The sixth axis is pH, the antioxidant abilities of acidic low molecular weight polysaccharides are better than acidic high molecular weight polysaccharides 43 . According to the structure–activity relationship mentioned before, it could be suggested that the antioxidant properties of BMEPS could be associated with its low molecular weight (4.31 × 10 4 g/mol), uronic acids (24.7%), sulfate (18.3%), monosaccharaides composition; mannuronic acid, glucouronic acid, glucose and mannose in a molar ratio of 0.8:1.0:2.8:2.3, respectively.…”

Section: Discussionmentioning

confidence: 99%

Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1

Selim

Mohamed

Asker

et al. 2023

Sci Rep

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Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetylcholinesterase inhibition. The isolate S16 provided the highest EPS yield (7.51 g/L) and acetylcholinesterase inhibition. It was identified morphologically and genetically using 16S rRNA gene sequence analysis as Bacillus maritimus. A Physicochemical analysis of S16 exopolysaccharide (BMEPS) was estimated, which pointed to the presence of uronic acid and sulfate (24.7% and 18.3%, respectively). HPLC analysis indicated that mannuronic acid, glucuronic acid, glucose, and mannose are presented in a molar ratio of 0.8:1.0:2.8:2.3, respectively. Furthermore, FT-IR revealed an abundance of β-configurations. The GPC estimated the average molecular weight (Mw) as 4.31 × 104 g/mol. BMEPS inhibited AChE (IC50; 691.77 ± 8.65 μg/ ml), BChE (IC50; 288.27 ± 10.50 μg/ ml), and tyrosinase (IC50; 3.34 ± 0.09, 14.00 ± 0.14, and 22.96 ± 1.23 μg/ ml during incubation durations of 10, 20, and 40 min). It also demonstrated a selective anti-inflammatory action against COX-2 rather than COX-1. Moreover, BMEPS exhibited antioxidant capabilities as free radical and oxygen reactive species (ROS) scavenger, metal chelator, reductant agent, and lipid peroxidation suppressor. These activities are due to the distinct chemical composition. The findings of this study indicate that BMEPS could be considered as promising anti-disease Alzheimer's (AD) material in an in-vitro model, which qualifies it for advanced in-vivo studies in the discovery of alternative Alzheimer’s treatment.

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Purification and neuroprotective effects of polysaccharides from Opuntia Milpa Alta in cultured cortical neurons

Cited by 15 publications

References 31 publications

Use of fluorescent ANTS to examine the BBB-permeability of polysaccharide

Use of fluorescent ANTS to examine the BBB-permeability of polysaccharide

Optimization of Extraction Process of Polysaccharides MAP-2 from Opuntia Milpa Alta by Response Surface Methodology and Evaluation of Its Potential as α-Glucosidase Inhibitor

Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1

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