Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676

Jin, Jian; Baek, Seung Ryel; Lee, Kyung Rim; Lee, Jung Kwan; Yun, Sung Hwan; Kang, Seogchan; Lee, Yin Won

doi:10.5423/ppj.2008.24.4.417

Cited by 11 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…incarnatum , also known by the synonyms F. pallidoroseum and F. semitectum , is often regarded as a secondary colonizer of plant tissues and causes several plant diseases including pod and collar rot in soybeans [35], soybean root rot [36], and postharvest fruit rot in oriental melon [37]. It produces apicidins phytotoxic to seedlings and 2-wk-old plants of diverse species [38] and is one of 11 pathogenic Fusarium species listed as quarantine pests in Korea [39]. In addition, it has also been isolated from rotten ginseng roots [5].…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Song

Yun

Kim

2014

Journal of Ginseng Research

View full text Add to dashboard Cite

BackgroundThis study aimed to develop a biocontrol system for ginseng root rot caused by Fusarium cf. incarnatum.MethodsIn total, 392 bacteria isolated from ginseng roots and various soils were screened for their antifungal activity against the fungal pathogen, and a bacterial isolate (B2-5) was selected as a promising candidate for the biocontrol because of the strong antagonistic activity of the bacterial cell suspension and culture filtrate against pathogen.ResultsThe bacterial isolate B2-5 displayed an enhanced inhibitory activity against the pathogen mycelial growth with a temperature increase to 25°C, produced no pectinase (related to root rotting) and no critical rot symptoms at low [106 colony-forming units (CFU)/mL] and high (108 CFU/mL) inoculum concentrations. In pot experiments, pretreatment with the bacterial isolate in the presumed optimal time for disease control reduced disease severity significantly with a higher control efficacy at an inoculum concentration of 106 CFU/mL than at 108 CFU/mL. The establishment and colonization ability of the bacterial isolates on the ginseng rhizosphere appeared to be higher when both the bacterial isolate and the pathogen were coinoculated than when the bacterial isolate was inoculated alone, suggesting its target-oriented biocontrol activity against the pathogen. Scanning electron microscopy showed that the pathogen hyphae were twisted and shriveled by the bacterial treatment, which may be a symptom of direct damage by antifungal substances.ConclusionAll of these results suggest that the bacterial isolate has good potential as a microbial agent for the biocontrol of the ginseng root rot caused by F. cf. incarnatum.

show abstract

Section: Discussionmentioning

confidence: 99%

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Song

Yun

Kim

2014

Journal of Ginseng Research

View full text Add to dashboard Cite

show abstract

“…The ability to produce apicidin is not widely distributed among Fusarium spp., including the F. semitectum – F. equiseti complex (Park et al ., 1999; Jin et al ., 2008). None of the fungal strains tested in this study produced apicidin, but some strains of F. asiaticum carry bands hybridized to APS genes.…”

Section: Discussionmentioning

confidence: 99%

“…The saprophytic fungus F. semitectum KCTC16676 isolated from soybeans in Korea (Park et al ., 1999; Jin et al ., 2008) was used as the wild‐type strain. Fungal stock cultures were stored in 20% glycerol at −80°C and activated on potato dextrose agar (PDA).…”

Section: Methodsmentioning

confidence: 99%

“…Certain isolates of F. semitectum produce cyclic tetrapeptides [cyclo‐( l ‐ N ‐ O ‐methyl‐tryptophanyl‐ l ‐isoleucinyl‐ d ‐pipecolinyl‐ l ‐2‐amino‐8‐oxo‐decanoyl)] named apicidin ( 1 ), apicidin D 2 ( 3 ) and apicidin B ( 4 ) (Fig. 1) (Singh et al ., 1996; 2001; 2002;Park et al ., 1999; Jin et al ., 2008). Apicidin exhibits broad‐spectrum antiprotozoal activity in vivo against Apicomplexan parasites.…”

Section: Introductionmentioning

confidence: 99%

“…The unique structure, mode of action and biological activity of apicidin have generated strong interest in the possibility of producing novel apicidin analogues by isolating strains with such capability and/or genetic modifications of the existing biosynthetic pathway (Singh et al ., 1996). In this study, we cloned and defined the genes involved in apicidin biosynthesis from an apicidin‐producing F. semitectum strain (Park et al ., 1999; Jin et al ., 2008). Reconstruction of the biosynthetic pathway via targeted mutagenesis of individual genes and chemical analyses of the resulting mutants allowed the engineering of this biosynthetic pathway to improve the production of apicidin and to produce new analogues.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional characterization and manipulation of the apicidin biosynthetic pathway inFusarium semitectum

Jin

Lee

et al. 2010

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

SummaryApicidin is a cyclic tetrapeptide produced by certain isolates of Fusarium semitectum and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin-producing strain (KCTC16676) of the filamentous fungus was mutated using an Agrobacterium tumefaciens-mediated transformation, resulting in 24 apicidin-deficient mutants. Three of the mutants had a T-DNA insertion in a gene that encodes a nonribosomal peptide synthetase (NRPS). Results of sequence, expression, and gene deletion analyses defined an apicidin biosynthetic gene cluster, and the NRPS gene was named as apicidin synthetase gene 1 (APS1). A 63 kb region surrounding APS1 was sequenced and analysis revealed the presence of 19 genes. All of the genes including APS1 were individually deleted to determine their roles in apicidin biosynthesis. Chemical analyses of the mutant strains showed that eight genes are required for apicidin production and were used to propose an apicidin biosynthetic pathway. The apicidin analogues apicidin E, apicidin D 2 and apicidin B were identified from chemical analysis of the mutants. The cluster gene APS2, a putative transcription factor, was shown to regulate expression of the genes in the cluster and overexpression of APS2 increased apicidin production. This study establishes the apicidin biosynthetic pathway and provides new opportunities to improve the production of apicidin and produce new analogues.

show abstract

An Endophytic Sanguinarine-Producing Fungus from Macleaya cordata, Fusarium proliferatum BLH51

Wang

Min

Ge³

et al. 2013

Curr Microbiol

View full text Add to dashboard Cite

Fermentation processes using sanguinarine-producing fungi other than Macleaya cordata may be an alternative way to produce sanguinarine (SA), which is a quaternary benzo[c]phenanthridine alkaloid possessing antibacterial, anthelmintic, and anti-inflammatory properties. In this study, a SA-producing endophytic fungus strain BLH51 was isolated from the leaves of M. cordata grown in the Dabie Mountain, China. Strain BLH51 produced SA when grown in potato dextrose liquid medium. The amount of SA produced by this endophytic fungus was quantified to be 178 μg/L by HPLC, substantially lower than that produced by the host tissue. The fungal SA--which was analyzed by thin layer chromatography and high-performance liquid chromatography--was shown to be identical to authentic SA. Strain BLH51 was identified as Fusarium proliferatum based on the morphological characteristics and nuclear ribosomal DNA ITS sequence analysis. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic SA-producing fungi from the host plant, which further proved that endophytic fungi are valuable reservoirs of bioactive compounds.

show abstract

Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676

Cited by 11 publications

References 19 publications

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Functional characterization and manipulation of the apicidin biosynthetic pathway inFusarium semitectum

An Endophytic Sanguinarine-Producing Fungus from Macleaya cordata, Fusarium proliferatum BLH51

Contact Info

Product

Resources

About