Purification and primary structure determination of a galactose-specific lectin from Vatairea guianensis Aublet seeds that exhibits vasorelaxant effect

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

“…The loss of activity of the lectin with increased temperature is due to destabilization of sporadic weak interactions of tertiary structure responsible for native conformation of lectin [39]. These results are comparable to the reported results of lectins purified from Vatairea marcocarpa [11], Vatairea guianensis [15], Platymiscium floribundum [16], and Canavalia oxyphylla [42]. In contrast, extremely thermostable lectins have been reported from Bauhinia forficate [43] and Apuleia leiocarpa [44].…”

“…These probably suggest that POSL does not need divalent cations for it to be active or the metal ions are tightly bound to the lectin. The hemagglutinating activity of lectin from Vatairea guianensis [15] and Platymiscium floribundum [16] among others showed similar results when incubated with EDTA. Although, this is in contrast to the P. angolensis [13] and Centrolobium microchaete [18] lectins that completely lost their activity after treatment with EDTA and only addition of metal ions restored their full lectin activity.…”

“…They are found to display maximum activity at around neutral pH. Lectins from Vatairea guianensis [15], Platymiscium floribundum [16] and Centrolobium microchaete [18] retained full hemagglutinating activity at pH 6-9. They all belong to the same Dalbergieae tribe with the P. osun.…”

“…Among well studied member of the tribe that belong to mannose/glucose specificity group are lectins from Pterocarpus angolensis [3], Platymiscium floribundum [16], Centrolobium microchaete [18], and Platypodium elegans [40]. Though, other members of the tribe that have specificity for other carbohydrates especially galactose have also been reported (Vatairea marcocarpa [11]; Lonchocarpus capassa [14]; Vatairea guianensis [15]). The biological importance of mannose-binding lectin also has been stretched [41].…”

“…Worthy of mention is Pterocarpus angolensis seeds lectin which has been purified and physicochemically, biochemically and structurally characterized [12,13]. Other seed lectins that have been purified and biochemically characterized from this tribe include galactosebinding lectins from Lonchocarpus capassa [14], Vatairea macrocarpa [11] and Vatairea guianensis [15] and mannose-binding lectins from Platymiscium floribundum [16] and Centrolobium tomentosum [17]. Amino acid sequence of P. floribundum [16], C. tomentosum [17] and Centrolobium microchate [18] lectins has been determined partially.…”