Purification and properties of a ribonuclease from avena leaf tissues

Wyen, N.V.; Udvardy, J.; Solymosy, Ferenc; Marrè, E.; Farkas, G. L.

doi:10.1016/0005-2744(69)90352-0

Cited by 30 publications

(4 citation statements)

References 25 publications

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“…Isolation and characterization of RNase from plant tissues have been carrited out by several workers (3,28,(31)(32)(33)(34). A general increase in RNase activity has been reported in virus infected plants (23) and in wheat leaves inoculated with wheat rust (25).…”

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Changes in the Ribonuclease Activity of Flax Cotyledons following Inoculation with Flax Rust

1972

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“…Mechanical injury of plant tissues has been shown to result in demonstrable increases in the activities of hydrolytic enzymes (2,34). The following experiments were designed to ascertain whether or not the observed increases in RNase activity of flax cotyledons ( Fig.…”

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Changes in the Ribonuclease Activity of Flax Cotyledons following Inoculation with Flax Rust

1972

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“…Those plant ribonucleases that have been studied exhibit no absolute base-specificity, but do show a marked preference for purine bases. They will hydrolyse purine cyclic nucleotides and are inhibited by purine mononucleotides (Wyen et al, 1969;L. Jervis, unpublished work).…”

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Purification of tobacco ribonuclease by affinity chromatography

Jervis

1972

Biochemical Journal

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Although it has long been considered that there may be specific proteins mediating the primary action of plant hormones, activity in this area has been relatively unproductive. Recently preliminary reports have indicated that, in the presence of certain protein fractions, auxins (Matthyse & Phillips, 1969) and cytokinins (Matthyse & Abrams, 1970) can stimulate RNA synthesis directed by isolated pea chromatin or DNA. Similarly it appears that both gibberellic acid (Johri & Varner, 1968) and abscisic acid (Pearson & Wareing, 1969) effects on RNA synthesis in vitro may require the presence of factors that are rapidly lost from nuclei or chromatin during the isolation procedures. An attempt has been made to obtain possible protein mediators of this kind by the affinity-chromatography principle, by using an agarose column to which a derivative of the synthetic auxin 2,4-dichlorophenoxyacetic acid is attached by the method of Cuatrecasas et al. (1968). Such a column retains small amounts of protein from crude extracts of pea or maize shoots. Elution with 2mM-KOH gives a protein factor that enhances DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 50-300 % in different preparations. Activity is not due to inhibition of adenosine triphosphatase or ribonuclease action, nor is it an endonuclease effect. Time-course experiments involving rifampicin suggest the factor acts, partially at least, on RNA chain initiation.

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“…β) Δεοξυριβονουκλεάσες που υδρολύουν μόνο το DNA και όχι το RNA, όπως είναι η ϋΝάση Ι στο πάγκρεας μόσχου (28) και η ϋΝάση της κριθής (3,207). γ) Ριβονουκλεάσες που υδρολύουν μόνο το RNA (αντίστροφα από τις προηγούμενες), όπως η παγκρεατική RNàcni Α (6) και οι φυτικές ΡΝασες Ι και II (123), για παράδειγμα οι ΡΝασες του καλαμποκιού (137,138) και η RNàoT) της βρώμης (151,153). II) Ανάλογα με το αν ο φωσφοδιεστερικός δεσμός που υδρολύεται βρί σκεται στο εσωτερικό ή στην άκρη της αλυσίδας, διακρίνονται σε : α) Ενδονουκλεάσες που προσβάλλουν φωσφοδιεστερικούς δεσμούς στο εσωτερικό του μορίου.…”

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Η Νουκλεολυτικη Δραση Σπερματων Βλαστημενης Φακης Καθαρισμος Και Μελε- Τη Ιδιοτητων Μιας Ενδονουκλεασης

Kefalas¹,

Κεφαλάς²

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Επιβλέπων καθηγητής Μέλος συμβουλευτικής επιτροπής Μέλος συμβουλευτικής επιτροπής Πανεπιστήμιο Πατρών Αριστοτέλειο Παν/μιο Θεσσαλονίκης Αριστοτέλειο Παν/μιο Θεσσαλονίκης Αριστοτέλειο Παν/μιο Θεσσαλονίκης ΘΕΣΣΑΛΟΝΙΚΗ 1995 ΠΕΡΙΕΧΟΜΕΝΑ ΣΥΝΤΜΉΣΕΙΣ Α. ΕΙΣΑΓΩΓΗ Α.1. Ιστορική αναδρομή στη μελέτη των νουκλεολυτικών ενζύμων Α.2. Δράση και μηχανισμός δράσης των νουκλεολυτικών ενζύμων Α.3. Κατάταξη των νουκλεολυτικών ενζύμων Α.4. Εξειδίκευση των νουκλεασών Α.4.1. Εξειδίκευση ως προς την πρωτοταγή δομή των νουκλεϊνικών οξέων Α.4.2. Εξειδίκευση απέναντι στις ανώτερες δομές των νουκλεϊνικών οξέων..

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Purification and properties of a ribonuclease from avena leaf tissues

Cited by 30 publications

References 25 publications

Changes in the Ribonuclease Activity of Flax Cotyledons following Inoculation with Flax Rust

Changes in the Ribonuclease Activity of Flax Cotyledons following Inoculation with Flax Rust

Purification of tobacco ribonuclease by affinity chromatography

Η Νουκλεολυτικη Δραση Σπερματων Βλαστημενης Φακης Καθαρισμος Και Μελε- Τη Ιδιοτητων Μιας Ενδονουκλεασης

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