Purification and Properties of Acid Phosphatase-1 from a Nematode Resistant Tomato Cultivar

Paul, E. M. M.; Williamson, Valerie M.

doi:10.1104/pp.84.2.399

Cited by 31 publications

(29 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…indicate that the native enzyme is a homo-dimer with a molecular weight of 72,000 for the subunit. This molecular weight of the homo-dimer was larger than that of other acid phosphatases purified from seeds of triticale (45,700 for monomer, Ching et al 1987), cell cultures of tomato (51,000 for dimer, Paul and Williamson 1987), barley roots (79,000 and 77,600 for dimer, Panara et al 1990), soybean axes and cotyledons (i00,000 for dimer, Kaneko et al 1990), seeds of sunflower (103,000 for dimer, Park and Etten 1986), tuberous root of sweet potato (110,000 and 105,000 for dimer, Uehara et al 1974), and cell culture of soybean (130,000 for dimer, LeBansky et al 1992), but smaller than that from cotton seedlings (200,000 for tetramer, Bhargava and Sachar 1987), and peanut seeds (240,000 for hexomer, Basha et al 1984). The isoelectric points (pI) of crude acid phosphatases from secretion, roots and leaves were determined by I E F (Fig.…”

Section: H a R A C T E R I S T I C S Of S E C R E T E D A C I D P Hmentioning

confidence: 99%

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Ozawa

Osaki

Matsui

et al. 1995

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

Section: H a R A C T E R I S T I C S Of S E C R E T E D A C I D P Hmentioning

confidence: 99%

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Ozawa

Osaki

Matsui

et al. 1995

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

“…The procedure developed here for purification of Apase-11 is considerably faster than previously reported methods (14,22). Because the procedure eliminates the ammonium sulfate precipitation and lengthy dialysis steps the purification can be accomplished in 3 d. Each chromatography step can be carried out in <2.5 h with minimal sample manipulation between steps.…”

Section: Purification Of Apase-11mentioning

confidence: 83%

“…Approximately 25 to 50 ,ug of pure Apase-1' was recovered from 1.5 kg of packed cells. The presence of other interfering acid phosphatases, which account for most of the activity present in cell extracts, prevents the determination of overall recoveries and fold purifications (14). The concentration of Apase-l1 in crude extracts was estimated by comparing the Apase-1 enzyme activity of crude and purified samples using the cellulose acetate gel electrophoresis procedure.…”

Section: Purification Of Apase-11mentioning

confidence: 99%

“…Purification of Apase-11 from Tomato Suspension Cells Tomato, Lycopersicon esculentum cv VFNT, cells were grown in 6-L flasks for 7 to 10 d with 1 L ofcomplete medium containing sucrose (14). Six flasks yield 1.5 (2).…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…Apase-1' activity was determined by a cellulose acetate gel electrophoresis procedure using f3-3-napthylphosphate as the substrate (14) and by a microtiter plate assay using p-nitrophenylphosphate as the substrate. Other conditions for enzyme assays were as previously described (14).…”

Section: Enzyme Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Characterization of a Tomato Acid Phosphatase Complementary DNA Associated with Nematode Resistance

Erion¹,

Ballo²,

May³

et al. 1991

Plant Physiol.

View full text Add to dashboard Cite

The tomato (Lycopersicon esculentum) acid phosphatase-1 (Apase-11, EC 3.1.3.2) isozyme variant, genetically linked to the root-knot nematode resistance locus (Mi) on chromosome 6, has been purified by a rapid procedure from tomato cell suspension cultures. Peptide fragments of the purified enzyme were generated from trypsin and Lys-C endoprotease digests and separated by reverse-phase high-performance liquid chromatography. Amino acid sequences derived from the purified peptide fragments represented >50% of the total amino acid content of the protein and enabled the construction of degenerate oligonucleotide probes that were used to screen a tomato cell culture complementary DNA library. Clones corresponding to full-length coding sequences for Apase-1 have been isolated and sequenced. Southem blot analysis of DNA isolated from a number of tomato cultivars shows that the Apase-11 gene (apsl) is present at one copy per genome and that genotypes containing the apsll allele have restriction fragment length polymorphisms that distinguish them from cultivars having the aps1+ allele. Segregation analysis demonstrates that the restriction fragment length polymorphisms are associated with the apsl locus. Tomato Apase-1' is also found to have significant homology at the amino acid sequence level to a class of vegetative storage proteins characterized in soybean.

show abstract

A rapid and efficient method for the screening of acid phosphatase 1 in young tomato seedlings, and for the identification of root‐knot nematode species using miniaturized polyacrylamide gel electrophoresis

Guillermo

Roberts

1992

Electrophoresis

View full text Add to dashboard Cite

A relatively rapid and highly sensitive miniaturized polyacrylamide gel electrophoresis technique is described for the analysis of certain isozymes from single cotyledons of tomato seedlings and from single females of the root-know nematode (Meloidogyne spp.). Homogenates from single tomato cotyledons (7, 14, 21, and 28 days old) were electrophoresed and stained for acid phosphatase 1 (Aps 1) activity. Cotyledons from plants of all the above age groups showed good Aps 1 activity. Nondestructive screening for tomato Aps 1 is therefore feasible, using very small samples, from as young as 7-day-old tomato seedlings. This could be of important use in expediting root-knot nematode resistance (based on the Aps 1-linked resistance gene Mi) screening for breeding programs, or F1 testing for seed production purposes. In addition, the mini-polyacrylamide gel electrophoresis technique was useful for determination of the Aps 1 allelic contribution to the total enzyme activity. The system was also used to detect malate dehydrogenase and esterase isozyme activity from single adult females of the four common root-knot nematodes, Meloidogyne arenaria, M. hapla, M. incognita, and M. javanica, with equally good results, enabling species discrimination.

show abstract

Purification and Properties of Acid Phosphatase-1 from a Nematode Resistant Tomato Cultivar

Abstract: In tomato the acid phosphatase-1 isozyme (Apase-1) is inherited as a single locus linked to the nematode resistance gene (Mi)

Cited by 31 publications

References 13 publications

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Isolation and Characterization of a Tomato Acid Phosphatase Complementary DNA Associated with Nematode Resistance

A rapid and efficient method for the screening of acid phosphatase 1 in young tomato seedlings, and for the identification of root‐knot nematode species using miniaturized polyacrylamide gel electrophoresis

Contact Info

Product

Resources

About