Purification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, Phaseolus vulgaris.

Marshall, J.J.; Lauda, C M

doi:10.1016/s0021-9258(19)40811-9

Cited by 255 publications

(46 citation statements)

References 35 publications

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“…Residues involved in ␣-AI1 binding to PPA Contrary to earlier enzymological studies [16], the present structural analysis reveals that ␣-AI is dimeric and interacts with two pancreatic enzyme molecules (PPA). Kinetics measurements of the inhibition of PPA by ␣-AI1 (P Rousseau and P Rougé, unpublished data) support the idea that ␣-AI1 ␣␤ units are themselves organized as dimers as seen in the crystal PPA-␣-AI1 structure, and that the structure observed here is therefore biologically relevant.…”

Section: Figurecontrasting

confidence: 94%

“…The last 19 residues at the C terminus are not seen in the electron density. The electron density enabled us to identify a third N-glycosylation site (five potential glycosylation sites are proposed in the literature [16]). All solvent molecules with densities below 1 in the (2F obs -F calc ) exp(i␣ calc ) map and temperature factors above 70 Å 2 were removed after the first iteration of refinement.…”

Section: Structure Determination and Refinementmentioning

confidence: 99%

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Substrate mimicry in the active center of a mammalian α amylase: structural analysis of an enzyme–inhibitor complex

et al. 1996

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The present analysis reveals that there are extensive interactions between the inhibitor and residues that are highly conserved in the active site of alpha-amylases; alpha-Al1 inactivates PPA through elaborate blockage of substrate-binding sites. It provides a basis to design peptide analogue inhibitors. alpha-Amylase inhibition is of interest from several points of view, for example the treatment of diabetes and for crop protection.

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Section: Figurecontrasting

confidence: 94%

Section: Structure Determination and Refinementmentioning

confidence: 99%

Substrate mimicry in the active center of a mammalian α amylase: structural analysis of an enzyme–inhibitor complex

et al. 1996

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“…Then, 200 µl of a 0.15% solution of potato starch was added and after 5 min at room temperature the reaction was stopped by adding 1 ml of Iodine reagent (Varner and Mense, 1972) and absorbance was measured at 620 nm. Results were expressed as units of α-amylase inhibited per mg of flour, where one unit of inhibitor activity is the amount which will bring about 50% inhibition of the α-amylase in 30 min under the above conditions according to Marshall and Lauda (1975).…”

Section: Assay Of α-Amylase Inhibitor Activitymentioning

confidence: 99%

The Challenge of Protein Crops as a Sustainable Source of Food and Feed for the Future

Ron¹,

Sparvoli²,

Pueyo³

et al. 2017

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial

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“…Em trabalho com o feijão branco, MARSHAL & LAUDA (1975), descreveram a purificação, bem como algumas propriedades físico-químicas do inibidor de alfaamilase, sendo denominado faseolamina. Entretanto, observou-se que a faseolamina possui alta especificidade pelas alfa-amilases do pâncreas de porcos, de seres humanos e também de saliva humana (MARSHALL & LAUDA, 1975).…”

Section: Revisão De Literaturaunclassified

BENEFÍCIOS DA FASEOLAMINA (Phaseolus vulgaris L.) - UMA REVISÃO

Colaço

Degáspari

2014

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Este trabalho relata sobre os benefícios da faseolamina, proteína extraída do feijão branco (Phaseolus vulgaris L.), grão este considerado um importante componente da dieta alimentar do brasileiro. A faseolamina possui efeito na inibição da digestão e também na absorção de amido, sendo, portanto uma inibidora da enzima alfa‑amilase. Ela tem sido utilizada como tratamento para indivíduos na perda de peso, bem como um efeito hipoglicemiante em diabéticos. A faseolamina é comercializada no Brasil na forma de cápsulas, que só podem ser manipuladas em farmácias magistrais, e a sua matéria-prima deve obtida de fornecedores que possuam laudo e certificado de análise que garantam sua qualidade. Em se tratando da posologia da faseolamina, os médicos prescrevem cápsulas com 500mg, administradas duas vezes ao dia. A recomendação para ingerir a faseolamina deve ser 30 minutos antes das duas principais refeições do dia. Nesta revisão de literatura foram considerados artigos científicos principalmente dos últimos cinco anos, com a finalidade de expandir a percepção dos benefícios e a aplicabilidade da faseolamina. A ingestão frequente desta proteína (através do consumo do feijão) impacta diretamente na qualidade de vida do brasileiro, pois o visível decréscimo no consumo de feijão é, indiretamente, uma das causas do aumento significativo da obesidade observado na população nos últimos anos.

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Purification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, Phaseolus vulgaris.

Cited by 255 publications

References 35 publications

Substrate mimicry in the active center of a mammalian α amylase: structural analysis of an enzyme–inhibitor complex

Substrate mimicry in the active center of a mammalian α amylase: structural analysis of an enzyme–inhibitor complex

The Challenge of Protein Crops as a Sustainable Source of Food and Feed for the Future

BENEFÍCIOS DA FASEOLAMINA (Phaseolus vulgaris L.) - UMA REVISÃO

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