Purification and properties of two forms of glucoamylase from Penicillium oxalicum.

Yamasaki, Yudai; Suzuki, Yukio; Ozawa, Junjiro

doi:10.1271/bbb1961.41.755

Cited by 42 publications

(19 citation statements)

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“…The conversion rates of glucose by the P. oxalicum enzyme and CTec2 at 10 mg/g were 68.2 and 59.2%, respectively. Similar to the results reported by Yamasaki et al 22) on secretion of a starch degrading enzyme by P. oxalicum, the culture supernatant of the present strain A592-4B as well as CTec2 degraded soluble starch (data not shown). The oligosaccharides in the degraded soluble starch constituted of more than three saccharides were less than detectable amount under the present experimental condition.…”

Section: Activity On Various Substratessupporting

confidence: 92%

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

Aoyama

Kurane

Matsuura

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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An enzyme producing micro-organism, which can directly saccharify rice straw that has only been crushed without undergoing the current acid or alkaline pretreatment, was found. From the homology with the ITS, 28S rDNA sequence, the strain named A592-4B was identified as Penicillium oxalicum. Activities of the A592-4B enzymes and commercial enzyme preparations were compared by Novozymes Cellic CTec2 and Genencore GC220. In the present experimental condition, activity of A592-4B enzymes was 2.6 times higher than that of CTec2 for degrading milled rice straw. Furthermore, even when a quarter amount of A592-4B enzyme was applied to the rice straw, the conversion rate was still higher than that by CTec2. By utilizing A592-4B enzymes, improved lignocellulose degradation yields can be achieved without pre-treatment of the substrates; thus, contributing to cost reduction as well as reducing environmental burden.

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Section: Activity On Various Substratessupporting

confidence: 92%

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

Aoyama

Kurane

Matsuura

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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show abstract

“…Though the recovery rate was relatively low, the purification steps were far more rapid than some other methods (Campos and Felix, 1995;James and Lee, 1997;Kleinman et al, 1988;Li et al, 1998;Martel et al, 2002;Tosi et al, 1993;Yamasaki et al, 1977).…”

Section: Discussionmentioning

confidence: 97%

Purification and characterization of a thermostable glucoamylase from Chaetomium thermophilum

Chen

Zhang

et al. 2005

J. Gen. Appl. Microbiol.

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“…The glucoamylase activity was measured as described by Yamasaki et al (1977) (w/v) maltose, 0.5 ml 1 M-acetate buffer (PH 5.3) and 0.3 ml enzyme solution (culture supernatant sample), was incubated at 40 "C for 10min. After incubation the reaction was stopped by heating in a boiling water bath.…”

Section: Methodsmentioning

confidence: 99%

Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided with multiple copies of the glaA gene

Schrickx

Krave

Verdoes³

et al. 1993

Journal of General Microbiology

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Continuous and recycling cultures were carried out with Aspergillus niger N402 wild-type and a glucoamylase overproducing transformant to investigate growth and product formation characteristics. In shake flask cultures, the amount of glucoamylase produced by the transformant was about five times more than by the wild-type strain. In contrast with these results, a twofold overproduction was found in glucose-limited continuous cultures, while no overproduction was found under maltodextin-limitation. Two regions of specific growth rates could be distinguished, one at specific growth rates lower (domain I) and one at specific growth rates higher than 0.12 h-' (domain 11). In domain I changes in mycelium morphology and conidia formation were observed. It has been concluded that maintenance requirements are dependent on the specific growth rate over the whole range of measured growth rates. The deviation in linearity in the linear equation of substrate utilization, caused by this phenomenon, should be considered when continuous cultures with filamentous fungi are performed. In recycling cultures, xylose as limiting carbon source repressed glucoamylase production very strongly. Under maltodextrinlimitation a fivefold overproduction was found. After about 150 h, the total amount of glucoamylase produced was still increasing, while total amount of product, measured as carbon, remained constant. After this time no increase in the amount of biomass formed was observed. These results suggest autolysis and cryptic growth taking place in a recycling fermenter and cell death rate equalling growth rate.

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Purification and properties of two forms of glucoamylase from Penicillium oxalicum.

Cited by 42 publications

References 0 publications

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

Purification and characterization of a thermostable glucoamylase from Chaetomium thermophilum

Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided with multiple copies of the glaA gene

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