Purification and Properties of Two Different Dihydroxyacetone Reductases in<i>Gluconobacter suboxydans</i>Grown on Glycerol

Adachi, Osao; Ano, Yoshitaka; Shinagawa, Emiko; Matsushita, Kazunobu

doi:10.1271/bbb.80199

Cited by 8 publications

(4 citation statements)

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“…The DHA phosphate may be converted to D-glyceraldehyde 3-phosphate by triosephosphate isomerase and thus enter into the glycolysis and gluconeogenesis pathway. However, the A. pasteurianus genome contains no genes encoding membrane-bound glycerol dehydrogenase which is well characterized in Gluconobacter (52), the capability to produce both DHA and glycerol 3-phosphate by G . xylinus (50,51) were reported and A. pasteurianus genome includes the glycerol kinase gene (described later) and several gens for alcohol dehydrogenase genes, such as sorbitol dehydrogenase.…”

Section: Resultsmentioning

confidence: 99%

Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus

Azuma

Hosoyama

Matsutani

et al. 2009

Nucleic Acids Research

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Acetobacter species have been used for brewing traditional vinegar and are known to have genetic instability. To clarify the mutability, Acetobacter pasteurianus NBRC 3283, which forms a multi-phenotype cell complex, was subjected to genome DNA sequencing. The genome analysis revealed that there are more than 280 transposons and five genes with hyper-mutable tandem repeats as common features in the genome consisting of a 2.9-Mb chromosome and six plasmids. There were three single nucleotide mutations and five transposon insertions in 32 isolates from the cell complex. The A. pasteurianus hyper-mutability was applied for breeding a temperature-resistant strain grown at an unviable high-temperature (42°C). The genomic DNA sequence of a heritable mutant showing temperature resistance was analyzed by mutation mapping, illustrating that a 92-kb deletion and three single nucleotide mutations occurred in the genome during the adaptation. Alpha-proteobacteria including A. pasteurianus consists of many intracellular symbionts and parasites, and their genomes show increased evolution rates and intensive genome reduction. However, A. pasteurianus is assumed to be a free-living bacterium, it may have the potentiality to evolve to fit in natural niches of seasonal fruits and flowers with other organisms, such as yeasts and lactic acid bacteria.

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Section: Resultsmentioning

confidence: 99%

Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus

Azuma

Hosoyama

Matsutani

et al. 2009

Nucleic Acids Research

Self Cite

167

145

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“…Since it is a nonfermentative carbon source for these bacteria its presence will result in increased biomass or delay the onset of stationary phase. A plaque is unlikely to increase in size as the lawn cells enter late log growth stage [10,37-39]. …”

Section: Discussionmentioning

confidence: 99%

The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique

et al. 2009

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BackgroundThe Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages and to isolate mutants and new phages. Many phages form large and well-defined plaques that are easily observed so that they can be enumerated when plated by the DLA technique. However, some give rise to small and turbid plaques that are very difficult to detect and count. To overcome these problems, some authors have suggested the use of dyes to improve the contrast between the plaques and the turbid host lawns. It has been reported that some antibiotics stimulate bacteria to produce phages, resulting in an increase in final titer. Thus, antibiotics might contribute to increasing plaque size in solid media.ResultsAntibiotics with different mechanisms of action were tested for their ability to enhance plaque morphology without suppressing phage development. Some antibiotics increased the phage plaque surface by up to 50-fold.ConclusionThis work presents a modification of the DLA technique that can be used routinely in the laboratory, leading to a more accurate enumeration of phages that would be difficult or even impossible otherwise.

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“…One of the two paralogs, the NBRC3255_0235 operon, is responsible for dihydroxyacetone production from glycerol as a glycerol dehydrogenase (9), and NBRC 3255 was shown to assimilate dihydroxyacetone (10). Although Prust et al suggested that the G. oxydans 621H strain assimilates dihydroxyacetone (7), we found that the strain fails to metabolize it (E. Kawajiri, M. Matsutani, T. Yakushi, O. Adachi, and K. Matsushita, unpublished data).…”

Section: Genome Announcementmentioning

confidence: 99%