Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2)

Oku, Yuichi; Yutsudo, Takashi; Hirayama, Toshiya; O'Brien, Alison D.; Takeda, Yoshifumi

doi:10.1016/0882-4010(89)90014-4

Cited by 66 publications

(48 citation statements)

References 26 publications

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“…It has been shown that Vero toxins share the same biological properties as Shiga toxin. The toxicities of the purified Shiga toxin and Vero toxins reported by several investigators differed considerably (32,54,63,72,83,100,101) probably because of different batches of toxins were studied and different animals and techniques were applied for the assays. Table 2 summarizes the biological activities of the purified published data).…”

Section: Structure Of the Vero Toxinsmentioning

confidence: 99%

Vero Toxins (Shiga‐Like Toxins) Produced by Enterohemorrhagic Escherichia coli (Verocytotoxin‐Producing E. coli)

Takeda

Kurazono

Yamasaki

1993

Microbiology and Immunology

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Section: Structure Of the Vero Toxinsmentioning

confidence: 99%

Vero Toxins (Shiga‐Like Toxins) Produced by Enterohemorrhagic Escherichia coli (Verocytotoxin‐Producing E. coli)

Takeda

Kurazono

Yamasaki

1993

Microbiology and Immunology

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“…Briefly, 96-well polystyrene microtiter plates (Maxisorp; Nalge Nunc International, Roskilde, Denmark) were coated with 100-l portions of purified Stx1 or Stx2 at a concentration of 2.5 g/ml in carbonate buffer (coating buffer), pH 9.6, or with a formalin-killed STEC whole-cell suspension diluted with coating buffer to an OD 600 of 0.01. Purified Stx1 and Stx2 were prepared as described previously (38,43,65). Intact STEC cells were prepared as follows.…”

mentioning

confidence: 99%

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

et al. 2001

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“…Purification procedures for the Stx I and Stx 2 were modified by the methods that had been reported (14,15,22) as below.…”

Section: Methodsmentioning

confidence: 99%

A Rapid Bioluminescent Enzyme Immunoassay (BLEIA) for the Detection of Shiga Toxin Types 1 and 2

Yamazaki

Sato

Gondaira

et al. 2001

Microbiology and Immunology

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In recent years, Escherichia coli 0157: 87 has emerged as a global public health concern. Among the more important virulence characteristics of this strain is its ability to produce one or more Shiga toxins (Stx). Traditional culture-based methods for assay of enteric toxins in foods and clinical samples are relatively slow and results can be ambiguous. In this work, we established a toxin-detection system based on bioluminescent enzyme immunoassay (BLEIA) using a simple and inexpensive device. The system could detect both Shiga toxin types 1 and 2 individually within 150 min with a detection limit for each toxin at 5 pglml. In our study of previously characterized Shigatoxigenic and all non-Shigatoxigenic E. coli and other bacterial species, we found all Shigatoxigenic strains to be positive and non-Shigatoxigenic E. coli and other bacterial species to be negative. This assay was also used to detect Stxs in milk and supernatant fluids from minced chicken and beef. For clinical stool samples we noted a tendency for the system to give unexpectedly high background level. Our results suggest the feasibility of using BLEIA methodology for the simple, rapid and sensitive detection of toxins from culture supernatant, various foods and clinical samples.

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Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2)

Cited by 66 publications

References 26 publications

Vero Toxins (Shiga‐Like Toxins) Produced by Enterohemorrhagic Escherichia coli (Verocytotoxin‐Producing E. coli)

Vero Toxins (Shiga‐Like Toxins) Produced by Enterohemorrhagic Escherichia coli (Verocytotoxin‐Producing E. coli)

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

A Rapid Bioluminescent Enzyme Immunoassay (BLEIA) for the Detection of Shiga Toxin Types 1 and 2

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