Purification and some properties of oat golden stripe virus

Abstract

S U M M A R YOat golden stripe virus (OGSV) was maintained in oats by mechanical inoculation and purified by extraction of leaves in borate buffer, two cycles of centrifugation through sucrose cushions and isopycnic centrifugation in CsCI. An antiserum with a titre of 1/1024 in precipitin tests was prepared. Particle length distribution was bimodal with median values, respectively, of 150 and 300 nm from dip preparations. Measurements from immunosorbent electron microscopy (ISEM) and purified preparations showed that the particles had partially degraded during these procedures. The virus sedimented as two components of 168 S and 218 S and had a buoyant density of 1.321 g ~m -~.Four isolates of OGSV reacted with the antiserum. Antiserum to members and possible members of the furovirus group were tested in ISEM decoration tests and in ELISA. OGSV was related to soil-borne wheat mosaic virus but not to beet necrotic yellow vein virus, hypochoeris mosaic virus or potato mop-top virus.

Serological relationships between five fungally transmitted cereal viruses and other elongated viruses

“…PVM, RCVMV, WSBMV and BN \1\-G gave values greater than those of the controls. The relationship with WSBMV has already been reported (Adams et al 1988).…”

“…while there are also relationships between OGSV and WSBMV (Adams et al, 1988). There is, however, little information on relationships with viruses in other groups.…”

Differentiation of three whitefly-transmitted geminiviruses from the Republic of Yemen

“…Grids were viewed in a Jeol 1200EX electron microscope. Immunosorbent electron microscopy (ISEM) was performed as described by Adams et al [1988] using antisera raised to African cassava mosaic virus (kindly provided by M. Swanson, SCRI, Dundee, UK) or squash leaf curl virus (kindly provided by Dr J.K. Brown, Univ. of Arizona, Arizona, USA).…”

Fungally‐transmitted viruses of cereals in the UK1

“…It can be transmitted mechanically and has been purified and an antiserum prepared (Adams et al, 1988b). It can be transmitted mechanically and has been purified and an antiserum prepared (Adams et al, 1988b).…”