Purification, characterization and functional properties of exopolysaccharide from a novel halophilic Natronotalea sambharensis sp. nov.

Singh, Shweta; Sran, Kulwinder Singh; Pinnaka, Anil Kumar; Choudhury, Anirban Roy

doi:10.1016/j.ijbiomac.2019.06.080

Cited by 32 publications

(9 citation statements)

References 41 publications

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“…Namely, this strong band was accepted as the main character of EPS. The peaks at 621 cm −1 and 520 cm −1 (690–515 cm) corresponded to stretching of alkyl halides 38,58 . The FTIR spectra also revealed that there were no visible bands around 1230 and 980 cm −1 in FTIR spectra, indicating that EPS did not contain significant amounts of nucleic acids.…”

Section: Resultsmentioning

confidence: 97%

Production of water‐soluble sulfated exopolysaccharide with anticancer activity from Anoxybacillus gonensisYK25

Karadayi

Aykutoğlu

Arslan

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND Thermophilic bacteria are accepted as novel sources of exopolysaccharides (EPSs) with different biological activities. This study was conducted to test EPS production potential of thermophilic bacteria isolated from hot springs in Erzurum province (Turkey), to optimize EPS production from the best isolate in molasses medium, to characterize the produced EPS, and to investigate its anticancer activity. RESULTS Among 28 isolates, the best EPS producer isolate was found to be Anoxybacillus gonensis YK25 (GenBank No: MN865832). Under optimized culture conditions (initial pH 7.0, temperature 55 °C, molasses concentration 100 mL/L, peptone concentration 6 g/L, and incubation time 96 h), 4.4 g/L EPS was produced. Molecular weight of EPS was 102 kDa. Carbohydrate and protein contents of EPS were 91.4% and 4.1%, respectively. EPS was a heteropolysaccharide containing xylose (59%), sucrose (18%), glucose (16%), and galactose (5%). It had a high solubility in water and a moderate solubility in dimethyl sulfoxide (DMSO), but it was insoluble in polar solvents. FTIR analysis confirmed the presence of hydroxyl, carboxyl, amide, and sulphate groups. EPS showed anticancer activity against SHSY‐5Y, HT‐29, DU‐145, and A‐549 cell lines in a dose‐dependent manner. The IC50 values of EPS for A‐549, DU‐145, SHSY‐5Y, and HT‐29 cancer cells were 5.5, 9.61, 15.62, and 17.35 mg/mL, respectively. CONCLUSION The EPS production potential of A. gonensis, and the anticancer activity of the produced EPS were revealed. Furthermore, it was shown for the first time that the main monomer of bacterial heteropolysaccharides would be xylose. This study indicates that Anoxybacillus species may be a novel source of pharmaceutically‐important EPSs. © 2020 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 97%

Production of water‐soluble sulfated exopolysaccharide with anticancer activity from Anoxybacillus gonensisYK25

Karadayi

Aykutoğlu

Arslan

et al. 2020

J of Chemical Tech & Biotech

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“…A similar porous structure was also reported by the EPS of Lactobacillus plantarum YW11 and Natronotalea sambharensis sp. nov. ( Singh et al, 2019 , Wang et al, 2015a ). These previous reported studies concluded that because EPS has a more porous and concentrated distribution structure, it may increase its water retention capacity and retain more moisture, thereby expanding the physical properties of the product by generating a hydrated polymer with matrix-like properties and increasing viscosity.…”

Section: Resultsmentioning

confidence: 99%

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

et al. 2022

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“…Studies involving cultivation under laboratory conditions have identified and tested most of the currently known halophiles to obtain their exact characteristics [16,80,102,103]. Through the use of laboratory cultivation of pure bacterial cultures, new bioagents produced by halophiles can also be discovered [95,104,105]. However, these traditional cultivation-based methods do not always work well for halophiles.…”

Section: Experiments Strategies Used In Halophiles Researchmentioning

confidence: 99%

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

Lach

Jecz

Strapagiel

et al. 2021

Genes

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Halophiles, the salt-loving organisms, have been investigated for at least a hundred years. They are found in all three domains of life, namely Archaea, Bacteria, and Eukarya, and occur in saline and hypersaline environments worldwide. They are already a valuable source of various biomolecules for biotechnological, pharmaceutical, cosmetological and industrial applications. In the present era of multidrug-resistant bacteria, cancer expansion, and extreme environmental pollution, the demand for new, effective compounds is higher and more urgent than ever before. Thus, the unique metabolism of halophilic microorganisms, their low nutritional requirements and their ability to adapt to harsh conditions (high salinity, high pressure and UV radiation, low oxygen concentration, hydrophobic conditions, extreme temperatures and pH, toxic compounds and heavy metals) make them promising candidates as a fruitful source of bioactive compounds. The main aim of this review is to highlight the nucleic acid sequencing experimental strategies used in halophile studies in concert with the presentation of recent examples of bioproducts and functions discovered in silico in the halophile’s genomes. We point out methodological gaps and solutions based on in silico methods that are helpful in the identification of valuable bioproducts synthesized by halophiles. We also show the potential of an increasing number of publicly available genomic and metagenomic data for halophilic organisms that can be analysed to identify such new bioproducts and their producers.

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Purification, characterization and functional properties of exopolysaccharide from a novel halophilic Natronotalea sambharensis sp. nov.

Cited by 32 publications

References 41 publications

Production of water‐soluble sulfated exopolysaccharide with anticancer activity from Anoxybacillus gonensisYK25

Production of water‐soluble sulfated exopolysaccharide with anticancer activity from Anoxybacillus gonensisYK25

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

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