Purification, Characterization, and in Vitro Activity of 2,4-Di-tert-butylphenol from Pseudomonas monteilii PsF84: Conformational and Molecular Docking Studies

Abstract

A novel strain of Pseudomonas monteilii, PsF84, was isolated from tannery waste soil from Jajmau, Kanpur, India. 16S rRNA gene sequence phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as P. monteilii. An antifungal volatile organic compound (VOC) active against hyphal growth of Fusarium oxysporum (CIMAP-IMI-357464) in vitro was isolated from strain PsF84 by using chromatographic techniques. The molecular formula of the antifungal VOC was deduced to be C₁₄H₂₂O by EI-MS and 1D and 2D NMR spectral analysis. 2,4-Di-tert-butylphenol was found to be effective against an agriculturally important fungus, namely, F. oxysporum, in inhibiting spore germination and hyphal growth. Molecular docking analysis of 2,4-di-tert-butylphenol with β-tubulin further validated the potential of β-tubulin binding in F. oxysporum. Two residues of β-tubulin protein, HIS 118 and THR 117, showed hydrogen binding with ligand. To the authors' knowledge, this is the first report of antifungal VOC (2,4-di-tert-butylphenol) produced by P. monteilii PsF84 that can be a potent inhibitor of β-tubulin of F. oxysporum.

Effects of standardized stem bark extract of Mangifera indica L. in wistar rats with 2,4-dinitrophenylhydrazine-induced haemolytic anaemia

“…Report have shown that 1,2-benzenediol possess antimicrobials as well as antiplatelet 25,26 activity. Phenol, 2,4-bis (1, 1-dimethylethyl)-has also been reported in Scolopendra subspinispes with strong antioxidant activity, 28 Pereskia bleo with anticancer activity, 29 and strain of Pseudomonas monteilii PsF84 with antifungal activity, 30 Additionally, vitamin E identified two times at different retention times in this study is a well known antioxidant compound used for the treatment of various illnesses while N-Acetyl-Alpha-D-glucosamine has been used as safe alternative therapies for osteoarthritis,…”

2,4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp.

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Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging

“…2,4-ditert-butylphenol is a phenolic compound and a secondary metabolite in plants. It has been reported to exhibit strong antioxidant activity, anti-cancer activity, antifungal activity and antibacterial activity [59, 62–64]. It is cytotoxic to MCF7 cells (IC50 value of 5.75 μg/mL), KB cells (IC50 value of 0.81 μg/mL) and CasKi cells (IC50 value of 4.5 μg/mL).…”

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