Purification, characterization and mode of action of plantaricin K25 produced by Lactobacillus plantarum

Wen, Lim Sue; Philip, Koshy; Ajam, Noni

doi:10.1016/j.foodcont.2015.08.010

Cited by 91 publications

(55 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most bacteriocin isolated from LAB have narrow antimicrobial spectrum, showing antimicrobial activity against only gram‐positive bacteria. In order to overcome the narrow spectrum, bacteriocin with broad‐spectrum antimicrobial activity are more preferred (Wen, Philip, & Ajam, ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

Kalhoro

Visessanguan

Nguyen

et al. 2019

J Food Process Preserv

View full text Add to dashboard Cite

This study aimed to isolate bacteriocin‐like inhibitory substance (BLIS) producing strains from raw buffalo milk and characterize their probiotic and antimicrobial potential. The BT‐11 isolate was identified through 16S rDNA gene sequencing and recA gene amplification as Lactobacillus paraplantarum. The BT‐11 strain survived in low pH (2, 3) and bile (0.5, 1, and 2%) for 3 hr of incubation. The strain exhibited adhesion to Caco‐2 cells (69.85%), auto‐aggregation (89.74%), and hydrophobicity (81.76%). The BLIS produced by BT‐11 showed antibacterial activity against indicator bacteria including multidrug‐resistant pathogens. Time–kill kinetic analysis revealed the reduction in the growth of Staphylococcus aureus and Salmonella typhimurium (>3 Log CFU/ml) in time‐dependent manner. BLIS showed biopreservative effect in meat contaminated with Listeria monocytogenes. The results concluded that L. paraplantarum BT‐11 is potential probiotic strain with potential to produce BLISs. Practical applications Raw buffalo milk represents excellent source of unexplored potential probiotics with antimicrobial characteristics. In this study, the Lactobacillus paraplantarum BT‐11 was isolated from raw buffalo milk and its probiotic potential and bacteriocin‐like inhibitory substance (BLIS) was characterized. The results demonstrated that L. paraplantarum BT‐11 is promising potential probiotic. The BLIS produced by L. paraplantarum BT‐11 showed antagonistic activity against pathogens including multidrug‐resistant foodborne bacteria. BLIS also showed biopreservative potential in meat against Listeria monocytogenes. L. paraplantarum and its antimicrobial compound can be used as bio preservative agent in food safety applications.

show abstract

Section: Resultsmentioning

confidence: 99%

“…monocytogenes, E.coli, S. typhimurium, and S. aureus are shown in only gram-positive bacteria. In order to overcome the narrow spectrum, bacteriocin with broad-spectrum antimicrobial activity are more preferred (Wen, Philip, & Ajam, 2016).…”

Section: Mic and Mbc Of Blismentioning

confidence: 99%

Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

Kalhoro

Visessanguan

Nguyen

et al. 2019

J Food Process Preserv

View full text Add to dashboard Cite

show abstract

“…Both peptide concentrations used in this study for the inhibition (%) of fungal growth resulted in the same order of magnitude as those obtained in our study. Wen, Philip, and Ajam (2016) studied the mode of action of a bacteriocin with low molecular weight, isolated from L. plantarum K25. This antimicrobial peptide, named plantaricin K25, exhibited a broad spectrum of inhibitory activity against both Gram-positive and Gram-negative bacteria.…”

Section: Isolation and Identification Of The Antimicrobial Peptides Pmentioning

confidence: 99%

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Luz¹,

Saladino²,

Luciano³

et al. 2017

LWT - Food Science and Technology

View full text Add to dashboard Cite

a b s t r a c tFood spoilage caused by mycotoxigenic moulds represents an important problem in food security. The antimicrobial peptides are compounds of natural origin constituted by a variable number (5e100) of amino acids held together through peptide bonds. In this work, the cell free supernatants (CFSs) containing peptides obtained from four strains of LAB were lyophilized, filtered and tested to determine the antifungal activity against Aspergillus Parasiticus and Penicillium expansum. CFS obtained by Lactobacillus plantarum showed the highest inhibition activity. CFS was fractionated by size exclusion chromatography and injected into the liquid chromatography coupled to diode array detector. One of the recollected fractions resulted interesting for the presence of three peaks that were purified by the technique of the LC-DAD using a semi preparative C18 column. Finally, the antifungal activity of the purified peptides was studied against A. Parasiticus and P. expansum in liquid medium. The MALDI-TOF/TOF mass spectrometry was used for the peptides identification. The three purified peptides presented an amino acidic sequence identified by a bioinformatics program of SGADTTFLTK, LVGKKVQTF, and GTLIGQDYK. The first peptide purified reduced 58% and 73% the growth of P. expansum and A. parasiticus, respectively, in liquid medium after 48 h incubation.

show abstract

“…The purified peptide samples were mixed with 2X Tricine-SDS-PAGE Loading Buffer (CWBIO) at a 1:1 proportion, heated at 100 • C for 3-5 min, and centrifuged at 13,000× g for 2 min to remove precipitated impurities. Peptides were separated on a 16.5% gel and electrophoresed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with a Tris-Tricine buffer system as described previously [39]. Then, immunoblots were performed with mouse anti-His-tag monoclonal antibody, goat anti-mouse IgG (H+L), HRP, and ECL detection reagents.…”

Section: Tris-tricine Sds-page and Western Blottingmentioning

confidence: 99%

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System

Abbas

et al. 2019

Biomolecules

View full text Add to dashboard Cite

The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.

show abstract

Purification, characterization and mode of action of plantaricin K25 produced by Lactobacillus plantarum

Cited by 91 publications

References 29 publications

Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System

Contact Info

Product

Resources

About