Purification de particules virales associées à l'enroulement de la vigne et mise au point d'un protocole ELISA permettant leur détection

Zimmermann, Dieter R.; Walter, Benjamin von; Gall, Olivier Le

doi:10.1051/agro:19880809

Cited by 13 publications

(9 citation statements)

References 1 publication

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“…Potyviruses were isolated from diseased grapes and fully characterized (TANNE et al 1977). The early identification of closterovirus-like particles (NAMBA et al 1979) has been confirmed by many authors (CASTELLANO et al 1983, CONTI et al 1980, GuGERLi et al 1984, MIENE et al 1984, RosciGEiONE and GUGEREI 1986, ZEE et al 1987, ZiMMERMANN et al 1988. Occasionally, isometric particles have been found in leafroll diseased grapevines (GUGERLI et al 1984, CASTELLANO et al 1983), but also in non-affected grapevines (personal observation).…”

Section: Resumementioning

confidence: 94%

Characterization and Serological Detection of Four Closterovirus‐like Particles Associated with Leafroll Disease on Grapevine

Zimmermann¹,

Bass²,

Legin³

et al. 1990

Journal of Phytopathology

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Four serologically unrelated closterovirus-like particles (GLRV-1, GLRV-2, GLRV-3 and GLRV-4) were isolated in our laboratory from leafroll diseased grapevines. Polyclonal antibodies raised against these particles were useful for their characterization and their detection m infected plants.The coat proteins of these four serotypes were characterized by a SDS-PAGE after denaturation, followed by a transfer on nitrocellulose sheet and itnmunoprinting using the specific polyclonal antibodies. The capsid of GLRV-1, GLRV-2, GLRV-3 and GLRV-4 contains a single protein species with molecular weight of about 39 Kd, 26 Kd, 43 Kd and 36 Kd respectively. No serological relation was found between tbese four filamentous particles either by ELISA, immuno electron microscopy or immunoblotting experiments.Serological analysis of many grapevines originating from tbe Middle East and Europe sbowed a ver)' close association between the presence of GLRV-1, GLRV-2, GLRV-3 and GLRV-4 antigens, and leafroll symptoms on Vitis vinifera Pinot Noir. This association was confirmed by testing sytnptomless and diseased grapevines collected in the field, and by serological analysis of heat treated plants originally infected by GLRV-1 and GLRV-3, which are the most widespread antigens detected in leafroll infected grapevines. ZusammenfassungCharakterisierung und serologischer Nachweis von vier mit der Blattrollkrankheit der Rebe assoziierten Closteroviren Vier serologisch nicht verwandte Glosteroviren (GLRV-1, GLRV-2, GLRV-3 und GLRV-4) wurden aus blattrollkranken Reben isoliert. Polyklonale Antikorper, die gegen diese Partikel gewonnen wurden, konnten mit Erfolg zu deren Charakterisierung und Nacbweis in infizierten Pflanzen eingesetzt werden. U.S. Copyrigh, Clearance Center Code Su.e^eni: 093 1 -1 785/90/3003-0205S02.50/0 206 ZiMMERMANN, BASS, LEGIN and WALTER

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Section: Resumementioning

confidence: 94%

Characterization and Serological Detection of Four Closterovirus‐like Particles Associated with Leafroll Disease on Grapevine

Zimmermann¹,

Bass²,

Legin³

et al. 1990

Journal of Phytopathology

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“…For electron microscopy investigations, polyclonal antisera to other GLRaV-1, GLRaV-3 and GVA isolates were included. The former was obtained from Dr P. Gugerli ELISA tests were carried out with a few modifications, as described in literature (Clark & Adams, 1977;Gugerli et al, 1984;Rosciglione & Gugerli, 1986;Teliz et al, 1987;Zimmermann et al, 1988) and in accordance with the manufacturer's instructions. Duplicate samples were collected from each vine for two consecutive years.…”

Section: Antisera and Serological Assaysmentioning

confidence: 99%

**Grapevine leafroll‐associated viruses and grapevine virus A in selected Vitis vinifera cultivars in northern Italy**

Credi

Giunchedi

1996

Plant Pathology

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The occurrence of grapevine leafroll‐associated virus 1 (GLRaV‐1), grapevine leafroll‐associated virus 3 (GLRaV‐3) and grapevine virus A (GVA) was demonstrated in a viticultural region of northern Italy (Emilia‐Romagna) using immunoelectron microscopy. Virus incidence was subsequently assessed using ELISA. A total of 60.6% of the 150 clone selections tested, from 18 local Vitis vinifera cultivars, were found to be infected. ELISA did not reveal the presence of grapevine leafroll‐associated virus 2 (GLRaV‐2) or grapevine leafroll‐associated virus 5 (GLRaV‐5). GLRaV‐1, GLRaV‐3 and GVA were found individually and in various combinations. The most common findings were GLRaV‐1 alone (25.3%) and associated with GVA (33%). Serological data confirmed that the majority (91%) of the clones known to be affected by grapevine leafroll (GLR), on its own or in association with rugose wood (RW), contained viruses. On the other hand, where the RW phenomenon was present on its own, only 40% of these clones were ELISA‐positive. The implications for the biology of GLR and RW are discussed and the complex aetiology of these grapevine diseases is confirmed.

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“…Grapevines originating from INRA grapevine collections (Colmar, Montpellier, France) were found to be infected with GLRaV-1 particles, by a double-antibody sandwich ELISA (DAS-ELISA) using polyclonal antibodies (Zimmermann et al, 1988). Plants were either maintained in the greenhouse (Greif & Walter, 1997) or grown in the field.…”

Section: Virus Isolatesmentioning

confidence: 99%

Establishment of a relationship between grapevine leafroll closteroviruses 1 and 3 by use of monoclonal antibodies

Seddas¹,

Haidar²,

Greif³

et al. 2000

Plant Pathology

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Five stable hybridoma cell lines secreting monoclonal antibodies (Mabs) specific for GLRaV-1, one of the agents involved in the aetiology of grapevine leafroll disease, were produced by fusing a nonsecreting myeloma cell line with spleen cells from BALB/c mice immunized with purified GLRaV-1. The Mabs were characterized for their recognition of virus coat protein by DAS-ELISA and Western blotting. Mab (1G10) reacted specifically in ELISA, immunoelectron microscopy and immunoblotting with both GLRaV-1 and GLRaV-3 coat proteins. Mab 1C4 detected 25 of the 33 GLRaV-1 isolates, while Mab 1B7 reacted with 32 isolates including the eight isolates not recognized by Mab 1C4. Two of these hybridoma lines (2F11 and 2F3) are now used routinely for the immunodiagnosis of GLRaV-1.

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Purification de particules virales associées à l'enroulement de la vigne et mise au point d'un protocole ELISA permettant leur détection

Abstract: RÉSUMÉ

Cited by 13 publications

References 1 publication

Characterization and Serological Detection of Four Closterovirus‐like Particles Associated with Leafroll Disease on Grapevine

Characterization and Serological Detection of Four Closterovirus‐like Particles Associated with Leafroll Disease on Grapevine

**Grapevine leafroll‐associated viruses and grapevine virus A in selected Vitis vinifera cultivars in northern Italy**

Establishment of a relationship between grapevine leafroll closteroviruses 1 and 3 by use of monoclonal antibodies

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