Purification, identification and characterization of an esterase with high enantioselectivity to (S)-ethyl indoline-2-carboxylate

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

“…4F. 64,75,76 These results are also in accordance to the in silico protein-ligand interaction. Molecular docking simulations performed for p-NP-butyrate indicated that specic bonding of esterase enzyme in this study with p-NP-butyrate occurred at arginine68 and serine226 of esterase as shown in Fig.…”

Identification of Novel Alkaloids from Portulaca oleracea L. and Characterization of Their Pharmacokinetics and GLP-1 Secretion-Promoting Activity in STC-1 Cells

“…The enzymatic cyclization of l -phenylalanine is thought to be the second step in the synthesis . Indoline-2-carboxylic acid and methyl indoline-2-carboxylate would form racemates of the target compounds by reaction with ferulic acid and caffeic acid, respectively, and then chiral compounds 1 – 6 would be produced by esterase biocatalysis …”

AMWEst, a new thermostable and detergent-tolerant esterase retrieved from the Albian aquifer

“…Our results converge with much work on the characterization of esterase enzymes from thermophilic and hyperthermophilic microorganisms which can be cultivated such as Ureibacillus thermosphaericus (Samoylova et al 2018 ), Geobacillus sp. HBB-4 (Metin et al 2006 ), Bacillus licheniformis (Bhardwaj et al 2020 ), Thermotoga maritima (Levisson et al 2007 ), Geobacillus thermodenitrifcans NG80-2 (Curci et al 2019 ), Bacillus pumilus (Sharma et al 2016 ) and Bacillus aryabhattai B8W22 (Zhang et al 2019 ) especially for detergents, Triton X-100, CHAPS, Tween 20 and even from the construction of metagenomic libraries (Lewin et al 2016 ; Pereira et al 2017 ; Jayanath et al 2018 ; Li et al 2019 ). In many of the studies cited above, SDS detergent greatly reduces the enzymatic activity even at low concentrations.…”