Purification of a Bacteriocin from <i>Lactobacillus plantarum</i> ZJ217 Active Against Methicillin-Resistant <i>Staphylococcus aureus</i>

Zhu, Xuan; Shen, L. L.; Liu, Jiao; Zhang, Chen; Gu, Qing

doi:10.1515/ijfe-2014-0176

Cited by 8 publications

(11 citation statements)

References 36 publications

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“…Currently, natural and synthetic antifungal peptides have been suggested to induce cell death and exert an antifungal effect mainly by membrane pore formation or lysis followed by the loss of cytoplasm [45][46][47]. The expressed recombinant KRE9 protein in the supernatant was purified after undergoing affinity chromatography and was analyzed by (A) sodium dodecyl sulfate-polyacrylamide gel electrophoresis and (B) western blot.…”

Section: Discussionmentioning

confidence: 99%

The antifungal peptide CGA-N12 inhibits cell wall synthesis of Candida tropicalis by interacting with KRE9

Liu

Dong

et al. 2020

Biochemical Journal

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CGA-N12, an antifungal peptide derived from chromogranin A, has specific antagonistic activity against Candida spp., especially against Candida tropicalis, by inducing cell apoptosis. However, the effect of CGA-N12 on the Candida cell wall is unknown. The Candida protein KRE9, which possesses β-1,6-glucanase activity, was screened by affinity chromatography after binding to CGA-N12. In this study, the effect of CGA-N12 on KRE9 and the interaction between CGA-N12 and KRE9 was studied to clarify the effect of CGA-N12 on C. tropicalis cell wall synthesis. The effect of CGA-N12 on recombinant KRE9 β-1,6-glucanase activity was investigated by analyzing the consumption of glucose. The results showed that CGA-N12 inhibited the activity of KRE9. After C. tropicalis was treated with CGA-N12, the structure of the C. tropicalis cell wall was damaged. The interaction between CGA-N12 and KRE9 was analyzed by isothermal titration calorimetry (ITC). The results showed that their interaction process was involved an endothermic reaction, and the interaction force was mainly hydrophobic with a few electrostatic forces. The results of the fluorescence resonance energy transfer (FRET) assay showed that the distance between CGA-N12 and KRE9 was 7 ∼ 10 nm during their interaction. Therefore, we concluded that the target of CGA-N12 in the C. tropicalis cell membrane is KRE9, and that CGA-N12 weakly binds to KRE9 within a 7 ∼ 10 nm distance and inhibits KRE9 activity.

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Section: Discussionmentioning

confidence: 99%

The antifungal peptide CGA-N12 inhibits cell wall synthesis of Candida tropicalis by interacting with KRE9

Liu

Dong

et al. 2020

Biochemical Journal

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“…In this study, the L. plantarum D27 strain was found to inhibit the growth of E. coli ATCC 25922 and S. aureus ATCC 25923. Zhu et al (2015) reported that L. plantarum ZJ217 exhibited bacteriocinogenic activity against S. aureus and E. coli. According to Mulaw et al (2019), nine Lactobacillus strains showed varying degrees of antimicrobial activity against S. aureus, L. monocytogenes, S. Typhimurium and E. coli.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Investigation of some probiotic properties of Lactobacillaceae strains isolated from traditional Tulum cheese in Turkey

Kiliç Kanak

2023

Mljekarstvo

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The aim of this study was to evaluate the probiotic properties of Lactobacillaceae strains isolated from Tulum, Turkey. Ten strains of Lactobacillaceae spp. were taxonomically identified as follows: Lactiplantibacillus plantarum subsp. plantarum (6), Lacticaseibacillus paracasei (2), Lacticaseibacillus rhamnosus (1) and Lacticaseibacillus casei (1). Their probiotic properties, antimicrobial activities and tolerance to simulated gastrointestinal conditions such as low pH, pepsin, pancreatin and bile salts were evaluated. The results show that 10 lactic acid bacterial strains have high auto aggregation, coaggregation and hydrophobic properties. Antibiotic resistance and haemolytic activity were determined for safety assessment of the strains. Selected isolates showing high antimicrobial activity and the ability to remove cholesterol were also detected. These bacteria were found to have antioxidant activity as well. Therefore, these ten extracted lactic acid bacteria are promising potential candidates for use as probiotics. In this comprehensive study, L. plantarum SM27, L. plantarum S74 and L. paracasei RU39-7 were found to have the best probiotic properties. Important information was obtained on the probiotic and functional properties of Lactobacillaceae strains isolated from traditionally fermented Tulum cheese, which can be used as a rich source of probiotic bacteria. It was found that it is possible to isolate probiotic bacteria from traditional fermented cheeses.

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“…Tolworthi , was purified by liquid chromatography connected with a gel filtration column, resulting in 17% yield and a specific activity of about 3,600 U/mg protein (Pacheco‐Cano et al., 2014). Besides, affinity chromatography (Gibbs et al., 2004; Hechard, Derijard, Letellier, & Cenatiempo, 1992) and macroporous resin (Xu et al., 2019; Zhu et al., 2014; Zhu, Shen, Liu, Zhang, & Gu, 2015) were used for the purification of bacteriocins. Ion‐exchange technology includes anion exchange and cation exchange (Table 5 shows some ion‐exchange columns used in the purification process of bacteriocins).…”

Section: Purification and Identification Of Bacteriocinsmentioning

confidence: 99%

Mining, heterologous expression, purification, antibactericidal mechanism, and application of bacteriocins: A review

Cui

Luo

Wang

et al. 2020

Comp Rev Food Sci Food Safe

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Bacteriocins are generally considered as low‐molecular‐weight ribosomal peptides or proteins synthesized by G+ and G− bacteria that inhibit or kill other related or unrelated microorganisms. However, low yield is an important factor restricting the application of bacteriocins. This paper reviews mining methods, heterologous expression in different systems, the purification technologies applied to bacteriocins, and identification methods, as well as the antibacterial mechanism and applications in three different food systems. Bioinformatics improves the efficiency of bacteriocins mining. Bacteriocins can be heterologously expressed in different expression systems (e.g., Escherichia coli, Lactobacillus, and yeast). Ammonium sulfate precipitation, dialysis membrane, pH‐mediated cell adsorption/desorption, solvent extraction, macroporous resin column, and chromatography are always used as purification methods for bacteriocins. The bacteriocins are identified through electrophoresis and mass spectrum. Cell envelope (e.g., cell permeabilization and pore formation) and inhibition of gene expression are common antibacterial mechanisms of bacteriocins. Bacteriocins can be added to protect meat products (e.g., beef and sausages), dairy products (e.g., cheese, milk, and yogurt), and vegetables and fruits (e.g., salad, apple juice, and soybean sprouts). The future research directions are also prospected.

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Purification of a Bacteriocin from Lactobacillus plantarum ZJ217 Active Against Methicillin-Resistant Staphylococcus aureus

Cited by 8 publications

References 36 publications

The antifungal peptide CGA-N12 inhibits cell wall synthesis of Candida tropicalis by interacting with KRE9

The antifungal peptide CGA-N12 inhibits cell wall synthesis of Candida tropicalis by interacting with KRE9

Investigation of some probiotic properties of Lactobacillaceae strains isolated from traditional Tulum cheese in Turkey

Mining, heterologous expression, purification, antibactericidal mechanism, and application of bacteriocins: A review

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