Purification of a cell-associated hemagglutinin from<i>Shigella dysenteriae</i>type 1

Guhathakurta, B.; Sasmal, D.; Ghosh, Amit; Pal, Chiranjib; Datta, A.

doi:10.1111/j.1574-695x.1996.tb00271.x

Cited by 3 publications

References 6 publications

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Identification of a 19.3-kDa protein in MRHA-positiveEdwardsiella tarda: putative fimbrial major subunit

Sakai

Kanai

Osatomi

et al. 2003

FEMS Microbiology Letters

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The hemagglutinating properties of Edwardsiella tarda isolated from fish were investigated. Hemagglutination of E. tarda was not inhibited by D-mannose but was strongly inhibited by fetuin and N-acetylneuraminic acid. Extraction of hemagglutinating activity from bacterial cells was achieved using n-octyl-L-D-thioglucoside (NOTG), and the NOTG extracts were fractionated by sucrose density gradient ultracentrifugation. Sodium dodecyl sulfate^polyacrylamide gel electrophoresis analysis of the fractions revealed that a 19.3-kDa protein band appeared in the fractions exhibiting highest hemagglutinating activity. In an immunoblot analysis of NOTG extracts from 18 strains of E. tarda, the 19.3-kDa protein was detected only in the extracts possessing hemagglutinating activity. The predicted amino acid sequence of a 534-bp gene encoding the 19.3-kDa protein was identical to fimbrial subunit (FimA) of E. tarda by FASTA homology search. These findings suggest that fimbriae are implicated in the hemagglutination of E. tarda.

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Identification of a 19.3-kDa protein in MRHA-positiveEdwardsiella tarda: putative fimbrial major subunit

Sakai

Kanai

Osatomi

et al. 2003

FEMS Microbiology Letters

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Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

2014

Bacterial Adhesion to Animal Cells and Tissues

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Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model

et al. 2012

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The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythrocytes collected from rabbit, guinea pig, chicken, and sheep. Only rabbit and guinea pig erythrocytes showed positive HA reactions in most of the cases. On the basis of HA pattern, 4 strains from each serogroup were selected for in vivo colonization studies. Our results showed a positive correlation between HA activity and colonization ability of the strains belonging to different serogroups (groups A, B, C, and D) of Shigella. In all 4 serogroups, high HA titer was associated with greater intestinal colonization.

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Purification of a cell-associated hemagglutinin fromShigella dysenteriaetype 1

Cited by 3 publications

References 6 publications

Identification of a 19.3-kDa protein in MRHA-positiveEdwardsiella tarda: putative fimbrial major subunit

Identification of a 19.3-kDa protein in MRHA-positiveEdwardsiella tarda: putative fimbrial major subunit

Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model

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