Purification of a k‐Carrageenase from Marine Cytophaga Species

Sarwar, Golam; Matayoshi, Seiken; Oda, Hiroshi

doi:10.1111/j.1348-0421.1987.tb03148.x

Cited by 46 publications

(16 citation statements)

References 10 publications

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“…Furthermore, compared with carrageenan, carrageenan oligosaccharide and its derivants have more biological activities including anti-tumor [7], anti-oxidation [8], anti-cogulant, immunoregulation and anti-angiogenesis [9]. Among the previous reports, κ-carrageenases have been isolated from Pseudoalteromonas [10], Cytophaga [11], Alteromonas carrageenovora and Pseudomonas carrageenovora [12,13]. In the present study, a κ-carrageenase was purified from Cellulophaga lytica strain N5-2, and the hydrolyzed products of the enzyme were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products

Yao

Wang

Gao

et al. 2013

IJMS

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A carrageenan-degrading marine Cellulophaga lytica strain N5-2 was isolated from the sediment of carrageenan production base. A κ-carrageenase (EC 3.2.1.83) with high activity was purified to electrophoretic homogeneity from the culture supernatant by a procedure of ammonium sulfate precipitation, dialyzing and gel filtration on SephadexG-200 and SephadexG-75. The purified enzyme was verified as a single protein on SDS-PAGE, and whose molecular weight was 40.8 kDa. The κ-carrageenase yielded a high activity of 1170 U/mg protein. For κ-carrageenase activity, the optimum temperature and pH were 35 °C and pH 7.0, respectively. The enzyme was stable at 40 °C for at least 2.5 h. The enzyme against κ-carrageenan gave a Km value of 1.647 mg/mL and a Vmax value of 8.7 μmol/min/mg when the reaction was carried out at 35 °C and pH 7.0. The degradation products of the κ-carrageenase were analyzed by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), electrospray ionization time-of-flight mass spectroscopy (ESI-TOF-MS) and 13C-NMR spectroscopy, and the results indicated that the enzyme was specific of the β-1,4 linkage and hydrolyzed κ-carrageenan into κ-neocarraoctaose-sulfate and κ-neocarrahexaose-sulfate first, and then broke κ-neocarraoctaose-sulfate into κ-neocarrabiose-sulfate and κ-neocarrahexaose-sulfate.

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Section: Introductionmentioning

confidence: 99%

Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products

Yao

Wang

Gao

et al. 2013

IJMS

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“…Several carrageenan degrading bacteria Pseudomonas elongata [24], Cytophaga sp. [25], Cytophaga like bacterium [26][27][28] were found to produce carrageenases at pH range of 7-8 and few at different temperatures, ranging from 25 °C [15,29] to 37 °C [24,27] are reported. The P. aeruginosa ZSL-2 grows better, when the growth medium was supplemented with 1 % sodium chloride.…”

Section: Discussionmentioning

confidence: 99%

Increased Production of Carrageenase by Pseudomonas aeruginosa ZSL-2 Using Taguchi Experimental Design

Ziayoddin

Lalitha

Shinde

2014

ILNS

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The culture conditions for the production of carrageenase were optimized using one-factor-at-atime method combined with orthogonal array design. With the one-factor-at-a-time method revealed optimal conditions for carrageenase production were 24 h of fermentation period, 28 °C incubation temperature at pH 8.0 with NaNO 3 as nitrogen source and carrageenan as carbon source in MMS media. Further optimization of carrgeenase production by using orthogonal experimental design L 9 (3 4 ) with four factors, temperature, pH, NH 4 NO 3 and carrageenan with their relevant levels revealed optimised conditions for carrageenase production were temperature of 28 °C, pH 8.0, 2 g L -1 NaNO 3 and 2 g L -1 carrageenan. The order of the factors affecting the fermentation process was found to be temperature > pH > NaNO 3 > carrageenan. The temperature played a significant role on the carrageenase production. Higher carrageenase yield with activity of 0.542 ±0.045 U ml -1 was obtained in the optimised medium when compared to those of basal medium. Carrageenase hydrolysed products of carrageenan were identified by LC-ESI-MS as neocarrabiose, neocarrabiose-4 sulfate, neocarratetraose, neocarratetraose-4 sulfate, anhydrogalactose, galactose, galactose-4 sulphate and sulphate.

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“…LPLM f /30 °C/48 h/pH 7.53550 %/35 °C/36 h6.0>50 %/pH 5.0–9.0/35 °C/1 h75.5 kDaLi et al (2015)7 Cellulosimicrobium cellulans Kappa-carrageenan/37 °C/250 rpm/24 h/pH 7.530NR n 6.0NR n NR n Beltagy et al (2012)8 Cellulosimicrobium cellulans ZM g /37 °C/250 rpm/24 h/pH 7.537NR n 7.5NR n NR n Youssef et al (2012)9 Cellulophaga lytica strain N5-2Kappa-carrageenan/35 °C/20 h35>85 %/40 °C/pH 7.0/150 min7.0>80 %/pH 7.0/360 min/35 °C40.8Yao et al (2013)10 Cellulophaga sp. QY3Iota-carrageenan/25 °C/150 rpm/20 h/pH 7.050>80 %/50 °C/pH 7.0/60 min7.0>70 %/pH 5.0–10.6/720 min/4 °C48.3 kDaMa et al (2013)11 Cytophaga lk-C783ZM g having carrageenan/25 °C/150 rpm/72 h25 °C50 %/50 °C/pH 7.0/10 min7.6NR n 100 kDaSarwar et al (1983a, b, 1987)12 Cytophaga MCA-2ZM g having carrageenan/32 °C/150 rpm/36 h/pH 7.5280 %/55 °C/30 min7.232 %/pH 10.83/24 h30 kDaMou et al (2004)13Dsij strainZM g /22 °C/250 rpm/24–40 h40...…”

Section: Sources Of Carrageenasesmentioning

confidence: 99%

“…Carrageenases have been produced by submerged fermentation in most of the studies (Sarwar et al 1983a, b, 1987; Youssef et al 2012; Ziayoddin et al 2014). However, few attempts have been made for the production of mannosidases by solid state fermentation (SSF).…”

Section: Production Conditions and Propertiesmentioning

confidence: 99%

Bacterial carrageenases: an overview of production and biotechnological applications

Chauhan

Saxena²

2016

3 Biotech

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Carrageenan, one of the phycocolloids is a sulfated galactan made up of linear chains of galactose and 3,6-anhydrogalactose with alternating α-(1 → 3) and β-(1 → 4) linkages and further classified based on the number and the position of sulfated ester(s); κ-, ι- and λ-carrageenan. Enzymes which degrade carrageenans are called k-, ι-, and λ-carrageenases. They all are endohydrolases that cleave the internal β-(1–4) linkages of carrageenans yielding products of the oligo-carrageenans. These enzymes are produced only by bacteria specifically gram negative bacteria. Majority of the marine bacteria produce these enzymes extracellularly and their activity is in wide range of temperature. They have found potential applications in biomedical field, bioethanol production, textile industry, as a detergent additive and for isolation of protoplast of algae etc. A comprehensive information shall be helpful for the effective understanding and application of these enzymes. In this review exhaustive information of bacterial carrageenases reported till date has been done. All the aspects like sources, production conditions, characterization, cloning and- biotechnological applications are summarized.

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Purification of a k‐Carrageenase from Marine Cytophaga Species

Cited by 46 publications

References 10 publications

Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products

Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products

Increased Production of Carrageenase by <i>Pseudomonas aeruginosa</i> ZSL-2 Using Taguchi Experimental Design

Bacterial carrageenases: an overview of production and biotechnological applications

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