Purification of a Lethal Toxin of Edwardsiella tarda.

Suprapto, Hari; Hara, Takatoshi; Nakai, Toshiko; Muroga, Kiyokuni

doi:10.3147/jsfp.31.203

Cited by 23 publications

(20 citation statements)

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“…The pathogenic mechanism and the invasion pathways of E. tarda are not clear. Several virulence factors have been implicated, including haemolysins (Janda et al, 1991a ;Hirono et al, 1997), dermatoxins (Ullah & Arai, 1983), a lethal toxin (Suprapto et al, 1996), anti-phagocyte killing (Ainsworth & Dexiang, 1990), congo-red binding and serum resistance (Janda et al, 1991b). There has been no study to correlate virulence factors of fish isolates with virulence in fish.…”

Section: Introductionmentioning

confidence: 99%

Use of green fluorescent protein (GFP) to study the invasion pathways of Edwardsiella tarda in in vivo and in vitro fish models

et al. 2000

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Edwardsiella tarda is a fish pathogen that causes systemic infections in many food and ornamental fish. E. tarda PPD130/91 and PPD125/87 were selected as representatives of the virulent and avirulent groups, respectively, from eight fish isolates, and transformed with plasmids encoding either green fluorescent protein (pGFPuv) or blue fluorescent protein (pBFP2). Two host models were used to study the invasion pathway of E. tarda in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Use of green fluorescent protein (GFP) to study the invasion pathways of Edwardsiella tarda in in vivo and in vitro fish models

et al. 2000

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“…Strains highly virulent among fishes can be differentiated easily by serotyping; strains belonging to serotype A have high abilities to acquire iron and to produce a lethal toxin against Japanese eel Anguilla japonica and Japanese flounder Paralichtys olivaceus (lida and Wakabayashi, 1990;Suprapto et al, 1995Suprapto et al, , 1996. However, there is little information about the phylogenetic diversity among populations of E. tarda.…”

mentioning

confidence: 99%

Identification of Fish-pathogenic Strains Belonging to the Genus Edwardsiella by Sequence Analysis of sodB.

Yamada

Wakabayashi

1999

Fish Pathol.

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Abstract--Nucleotide sequences of an internal fragment of iron-cofactored superoxide dismutase gene (sodB) from members of the genus Edwardsiella were determined directly from amplified DNA fragments. Sequences obtained were all 454 bp in length, and there was no insertion or deletion against that of Escherichia coli. Identical sequences were obtained for the three Edwardsiella ictaluri strains, the four atypical Edwardsiella tarda strains isolated from red sea bream Chrysophrus major, and the five strains of Edwardsiella sp. from Japanese eel Anguilla japonica, respec tively. In contrast, five classes of sodB sequences were found in 26 biochemically typical E. tarda strains and were assigned to sodB types 1 to 5. A phylogenetic tree based on the sodB sequences reveals that members of the genus Edwardsiella can be divided into two clusters, I and II, which differed from each other in their pathogenicity to fish. Cluster I is composed of the patho genic E. tarda strains isolated from Japanese eel, Japanese flounder Paralichtys olivaceus, nile tilapia Oreochromis niloticus, and ayu Plecoglossus altivelis, atypical E. tarda from red sea bream, Edwardsiella sp. from Japanese eel, and E. ictaluri, whereas cluster II is composed of the non-pathogenic E. tarda strains and Edwardsiella hoshinae. These data indicated that sequence analysis of sodB is an effective tool for identifying fish-pathogenic strains belonging to the genus Edwardsiella.

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“…Although the mechanism of pathogenicity of E. tarda is still largely unknown, some virulence factors related to the pathogenicity of this organism have been reported: these include the ability to invade and replicate in epithelial cells, such as HeLa and Hep-2 (Marques et al 1984, Janda et al 1991, Okuda et al 2008, and in phagocytic cells, such as fish phagocytes and murine macrophages (Srinivasa Rao et al 2001, Okuda et al 2006, and the ability to produce catalases, hemolysins, and dermato-or lethal toxins (Srinivasa Rao et al 2003a, Janda & Abbott 1993, Ullah & Arai 1983, Suprapto et al 1996. The weapon possessed by many pathogenic bacteria, the Type III secretion system (TTSS), was also identified as a very important virulence factor in E. tarda (Tan et al 2002, Srinivasa Rao et al 2003b, 2004.…”

Section: Introductionmentioning

confidence: 99%

ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2

Okuda

Takeuchi²,

Yasuda³

et al. 2016

Dis. Aquat. Org.

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The Type III secretion system (TTSS) is essential for the intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the TTSS gene cluster is required for intracellular replication and virulence of E. tarda. Here, we show that under TTSS-inducing conditions, the protein ORF13 was secreted into culture supernatant. Then, using a yeast 2-hybrid screen, we show that the mammalian factor Cugbp2, which regulates apoptosis in breast cancer cells, directly interacts with ORF13. A pull-down assay revealed that ORF13 binds to the C-terminal region of Cugbp2. Our results suggest that ORF13 may facilitate E. tarda replication in phagocytes by binding to Cugbp2.KEY WORDS: Edwardsiella tarda · Type III secretion system · ORF13 · Mammalian factor Cugbp2Resale or republication not permitted without written consent of the publisher

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Purification of a Lethal Toxin of Edwardsiella tarda.

Cited by 23 publications

References 9 publications

Use of green fluorescent protein (GFP) to study the invasion pathways of Edwardsiella tarda in in vivo and in vitro fish models

Use of green fluorescent protein (GFP) to study the invasion pathways of Edwardsiella tarda in in vivo and in vitro fish models

Identification of Fish-pathogenic Strains Belonging to the Genus Edwardsiella by Sequence Analysis of sodB.

ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2

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