Purification of a modified cyclosporine A by co-current centrifugal partition chromatography: Process development and intensification

Amarouche, Nassima; Boudesocque, Leslie; Sayagh, Charlotte; Giraud, Matthieu; McGarrity, John F.; Butté, Alessandro; Marchal, Luc; Foucault, Alain; Renault, Jean‐Hugues

doi:10.1016/j.chroma.2013.08.053

Cited by 16 publications

(6 citation statements)

References 11 publications

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“…Amarouche et al. report in their study a successful purification of an 11mer hydrophobic synthetic modified cyclosporine, showing an anti‐HIV activity. A biphasic solvent system composed of heptane/ethyl acetate/acetone/methanol/water was used.…”

Section: Applications For Centrifugal Partition Chromatographymentioning

confidence: 99%

“…And finally, there is no loss of chromatographic resolution during the scale‐up . The greatest disadvantage of this liquid–liquid chromatographic technique is, nevertheless, inevitable optimization of the separation conditions for each new sample . These all advantages and disadvantages can be summarized in several points: Advantages of CCC in general over LC with a solid support –There is no need to buy new and expensive preparative column, all that is needed are solvents (and additives if needed). –There is no need of sample pretreatment – there is no risk of destroying the solid expensive support (bead).…”

Section: Advantages and Disadvantages Of Centrifugal Partition Chromamentioning

confidence: 99%

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Centrifugal partition chromatography - A review of recent applications and some classic references

Bojczuk

Żyżelewicz

Hodurek³

2017

J. Sep. Sci.

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Countercurrent chromatography, based on liquid-liquid partitioning, has many technological variants. One of them is centrifugal partition chromatography, introduced by Wataru Murayama and Kanichi Nunogaki in 1982. This technique, like other countercurrent chromatography techniques, is based on the phenomenon of liquid-liquid partitioning between two immiscible liquid phases that stay at equilibrium. But the significant difference between this technique and others is the retention mechanism of stationary phase. In the case of centrifugal partition chromatography, this mechanism is based on hydrostatic force, formed by the centrifugal field in the rotor in one-axis centrifuge. Sometimes that allows more control of stationary phase, for example, when aqueous two-phase and other difficult solvent systems are used. However, the efficiency of the separation in centrifugal partition chromatography is also affected by a variety of parameters dependent on the sample properties in the solvent system, physical properties of the solvent system, parameters of the instrument, and the method. This article includes also recent ideas for improvements to the technique and broadening its application (e.g., (multiple) dual-mode or elution-extrusion procedure, pH-zone-refining centrifugal partition chromatography, ion-exchange centrifugal partition chromatography, online and offline coupling of centrifugal partition chromatography).

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Section: Applications For Centrifugal Partition Chromatographymentioning

confidence: 99%

Section: Advantages and Disadvantages Of Centrifugal Partition Chromamentioning

confidence: 99%

Centrifugal partition chromatography - A review of recent applications and some classic references

Bojczuk

Żyżelewicz

Hodurek³

2017

J. Sep. Sci.

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“…CPC further allows for rapid and inexpensive method development, higher throughput, higher yields and reduced costs compared to typical preparative HPLC techniques [33]. Based on these unique advantages and the high resolution offered by CPC, this technique has been applied for the separation of numerous bioactive natural products including sesamin, catharanthine and vindoline [34,35,36,37,38] as well as certain mycotoxins [39,40,41,42,43]. For example, fumonisin B 1 was separated from the crude extract of Fusarium verticillioides by CPC in a single 70-min run with a purity above 98% and total yield of 68% [39].…”

Section: Introductionmentioning

confidence: 99%

Separation and Purification of Aflatoxins by Centrifugal Partition Chromatography

Endre

Hegedűs

Turbat

et al. 2019

Toxins

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Aflatoxins are mycotoxins that are produced by several species of filamentous fungi. In the European Union, the concentration limits for this group of mycotoxins in food and feed products are very low (on the order of parts per billion). Thus, relatively high amounts of these substances in their pure forms are required as reference standards. Chromatographic techniques based on solid stationary phases are generally used to purify these molecules; however, liquid–liquid chromatographic separations may be a promising alternative. Therefore, this study proposes a liquid–liquid chromatographic method for the separation of four aflatoxins and impurities. To optimise the method, numerous biphasic solvent systems (chloroform-, acetone- and acetic acid-based systems) were tested and evaluated in terms of their effectiveness at partitioning aflatoxins; the toluene/acetic acid/water (30:24:50, v/v/v/%) system was found to be the most efficient for application in centrifugal partition chromatographic instrument. Using liquid–liquid instrumental separation, the four aflatoxins, namely B1 (400 mg), B2 (34 mg), G1 (817 mg) and G2 (100 mg), were successfully isolated with 96.3%–98.2% purity from 4.5 L of Aspergillus parasiticus fermented material in a 250 mL centrifugal partition chromatography column. The identities and purities of the purified components were confirmed, and the performance parameters of each separation step and the whole procedure was determined. The developed method could be effectively used to purify aflatoxins for analytical applications.

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“…Finally, there have been excellent studies on optimising the injection step when scaling up for production using centrifugal partition chromatography (CPC) [21] and then putting it into practice [22]. It should be noted that CPC is considered overall as a hydrostatic process (despite hydrodynamic cascade mixing in each chamber) as when the flow stops the stationary phase remains trapped in each chamber.…”

Section: Introductionmentioning

confidence: 99%

Sample injection strategy to increase throughput in counter-current chromatography: Case study of Honokiol purification

Peng

Hewitson

et al. 2016

Journal of Chromatography A

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Counter-current chromatography (CCC) has been widely used as a preparative separation method to purify natural products from plant extracts and fermentation broths. Traditionally, throughput optimization in CCC has focused on sample concentration and sample volume. In this paper sample injection was considered as consisting of three variables: injection flow rate, post-injection flow rate and sample solvent. The effects of these parameters were studied using a honokiol purification from a Magnolia officinalis bark extract as a case study aiming to achieve the highest throughput/yield ratio for greater than 99% purity of this potential anti-cancer drug obtained for submission to the Chinese FDA. An injection method was established that increased the throughput of honokiol by 46.5% (from 3.05g/h to 4.47g/h), and decreased the solvent consumption of mobile phase and stationary phase per gram of honokiol by 40.0% (from 0.68L/g to 0.41L/g) and 48.4% (from 0.40L/g to 0.21L/g) respectively. These results show the importance of understanding the whole injection process when optimizing a given CCC separation.

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Purification of a modified cyclosporine A by co-current centrifugal partition chromatography: Process development and intensification

Cited by 16 publications

References 11 publications

Centrifugal partition chromatography - A review of recent applications and some classic references

Centrifugal partition chromatography - A review of recent applications and some classic references

Separation and Purification of Aflatoxins by Centrifugal Partition Chromatography

Sample injection strategy to increase throughput in counter-current chromatography: Case study of Honokiol purification

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