Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis

Nopparatana, Chamnong; Setasuban, Prasert; Chaicumpa, Wanpen; Tapchaisri, Pramuan

doi:10.1016/0020-7519(91)90079-m

Cited by 54 publications

(38 citation statements)

References 9 publications

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“…14 The same antigen was also found in the somatic extract of G. spinigerum L3 collected from eels 27 and was confirmed by using a monoclonal antibody against the protein. 28 Larvae from G. spinigerum (recovered from infected mice) also contained the 24-kDa antigen, which was localized mainly in the esophagus and intestine of L3 larva of G. spinigerum.…”

Section: Discussionmentioning

confidence: 78%

Identification of Immunodominant Peptides from Gnathostoma binucleatum

Campista-León

Delgado‐Vargas²,

Landa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of Gnathostoma binucleatum and in Asia mainly by G. spinigerum. The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from G. binucleatum, somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.

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Section: Discussionmentioning

confidence: 78%

Identification of Immunodominant Peptides from Gnathostoma binucleatum

Campista-León

Delgado‐Vargas²,

Landa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…For the diagnosis of G. spinigerum infections, a G. spinigerum-AL3-antigen-based immunoblot, showing excellent sensitivity and specificity, 5 is currently the diagnostic method of choice. This assay is available in reference laboratories in Asia and Europe.…”

Section: Discussionmentioning

confidence: 99%

“…13,14,17 Antigen extraction from larvae, antigen preparation, and immunoblotting were performed according to published protocols. 5,6 We cross-tested positive and negative clinical reference sera as well as three sera from patients with suspected American gnathostomiasis in both assays. The three patients' sera were sent to the Swiss TPH reference laboratory for human parasitic diseases from the United States, Chile, and Sweden.…”

Section: Methodsmentioning

confidence: 99%

“…The detection of a G. spinigerum-specific 24-kDa band in this assay is considered diagnostic of gnathostomiasis. 5,6 In the absence of commercially available test kits, serological testing is currently restricted to several research institutions that offer diagnostic services (e.g., Hospital for Tropical Diseases, Mahidol University, Bangkok, Thailand; Swiss Tropical and Public Health Institute [Swiss TPH], Basel, Switzerland).…”

Section: Introductionmentioning

confidence: 99%

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Cross-Reactivity Pattern of Asian and American Human Gnathostomiasis in Western Blot Assays Using Crude Antigens Prepared from Gnathostoma spinigerum and Gnathostoma binucleatum Third-Stage Larvae

Neumayr

Ollague

Bravo

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is a zoonotic parasitosis endemic in many Asian and some Latin American countries. Most human infections are caused by Gnathostoma spinigerum in Asia and Gnathostoma binucleatum in the Americas, and recently, imported cases have been increasing among travelers returning from endemic regions. Confirmation of the clinical diagnosis relies largely on serologic tests, with a G. spinigerum-antigen-based immunoblot currently being the diagnostic method of choice. However, we repeatedly experienced that sera from patients with clinically suspected American gnathostomiasis gave negative results in this assay. Therefore, we used homologous methods to prepare G. spinigerum-and G. binucleatum-antigen-based immunoblot assays, and evaluated the cross-reactivity of the two assays. The results show incomplete cross-reactivity between the two assays: the G. spinigerum-antigen-based immunoblot apparently only detects Asian gnathostomiasis caused by G. spinigerum, whereas the G. binucleatum-antigen-based immunoblot is apparently capable of detecting American as well as Asian gnathostomiasis.

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“…Am sensitivsten und spezifischsten ist derzeit ein ELISA mit Verwendung eines Antigens aus Larven von Gnathostoma spinigerum im dritten Stadium. Kreuzreaktionen sind mit Angiostrongyliasis cantonensis und Paragonimiasis möglich, bei anderen Parasitosen, insbesondere Filariosen, jedoch nicht beobachtet worden (7,13,19). Diese Untersuchung wird derzeit weltweit nur von wenigen spezialisierten Zentren durchgeführt.…”

Section: Gnathostomiasis After a Trip To Thailandunclassified