Purification of Highly Radioactive Mouse Interferon Produced by Ehrlich Ascites Tumour Cells Induced by Sendai Virus

Dussaix, Élisabeth; Grégoire, Anne; Chany, C; Thang, D.C.; Thang, M.N.

doi:10.1099/0022-1317-64-2-285

Cited by 22 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rat interferon (rat IFN) was produced in newborn rat kidney cells modified by Kirshten virus (NRK Ki Msv), and was induced by Sendai virus (3.5 UHA/5 x 104 cells) in the laboratory of Professor C. Chany (Paris, France) following a modification of the methods of SLATTERY [18] and DUSSAIX [19]. It had a specific activity of 1.82 x 10 ~ IU/mg of protein against vesicular stomatitis virus (C. Chany, personal communication).…”

Section: Materials and Methods Substancesmentioning

confidence: 99%

Effect of rat andβ-human interferons on hyperacute experimental allergic encephalomyelitis in rats

Hertz¹,

Deghenghi²

1985

Agents and Actions

View full text Add to dashboard Cite

Human interferon-beta (human IFN-beta) and rat interferon (rat IFN) were evaluated on experimental allergic encephalomyelitis (EAE) in rats, a delayed cellular reaction resembling human multiple sclerosis (MS). Rat IFN was active by intravenous and intracerebroventricular routes. It decreased the severity of clinical symptoms of paralysis during the 22 days of the assay. Human IFN-beta, on the contrary, had no effect when similarly tested in this rat model. Cyclophosphamide delayed the onset of paralysis, but levamisole enhanced the severity of the EAE.

show abstract

Section: Materials and Methods Substancesmentioning

confidence: 99%

Effect of rat andβ-human interferons on hyperacute experimental allergic encephalomyelitis in rats

Hertz¹,

Deghenghi²

1985

Agents and Actions

View full text Add to dashboard Cite

show abstract

“…Purified mouse IFN (Dussaix et al, 1983), with a specific activity of 106 IU/mg protein, was used at a concentration of 104 IU/ml. As a control, human leukocyte IFN (5 x 104 IU/ml), which provoked neither antiviral effect, steroid stimulation nor cell rounding, was employed.…”

Section: Methodsmentioning

confidence: 99%

Factors Involved in Interferon-induced or Cholera Toxin-induced Steroidogenesis in Y-1 Mouse Adrenal Tumour Cells

Matsuguchi¹,

Moreau²,

Rousset³

et al. 1985

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

SUMMARYIn addition to its antiviral effect, interferon, at high concentrations, stimulates steroidogenesis and provokes cell rounding in Y-1 mouse adrenal tumour cells. This stimulation was inhibited by cytochalasin B and colchicine. In contrast, dibutyryl cAMP and cholera toxin, also able to induce steroid production and cell rounding, increased steroid production even in the presence of these cytoskeleton-disrupting agents. The initial trigger for interferon or cholera toxin thus probably involves a distinct receptor organization. However, since both inducers increased cAMP synthesis in this differentiated cell line, the further metabolic steps of ketosteroid production could be the same.

show abstract

“…Interferons, including murine IFN-alP, were prepared from Ehrlich ascites tumor (EAT) cells (Dussaix et al, 1983), at sp. act.…”

Section: Serummentioning

confidence: 99%

Sarcolectin and interferon in the regulation of cell growth

Jiang

Chany

1990

Journal Cellular Physiology

View full text Add to dashboard Cite

Sarcolectin is an endolectin present in a great variety of conjunctival tissues (muscles, cartilage, sarcomas), but also in brain or placental extracts of vertebrates, including primates. When purified to electrophoretical homogeneity as a 65-kd protein, it agglutinates cells and has an affinity for simple sugars. In addition, it is able to inhibit the synthesis of interferon (IFN)-dependent secondary proteins and to restore cells to their status ad primum. The biological effect of Poly(I).Poly(C)-induced feedback interferon is inhibited by the addition of sarcolectins, which also abolishes cellular refractoriness to repeated IFN induction. Similarly, sequential association of, first, Poly(I).Poly(C); 4-5 h later, sarcolectin restores the full capacity of both to promote cell growth, unrestrained by IFN. Indeed, the secondary proteins which are in the process of being synthesized are inhibited. In a great variety of animal cells, sarcolectin can also initiate growth after it has been blocked by IFN. This is not an all-or-none effect, but a balance may be struck by IFN and sarcolectin, depending on their respective concentrations and specific activity. We propose that the coordination of these cellular functions of Poly(I).Poly(C), IFN, and sarcolectin takes place in the form of a triangular growth-regulatory cycle and postulate that they thus maintain a balance during differentiated normal tissue development.

show abstract

Purification of Highly Radioactive Mouse Interferon Produced by Ehrlich Ascites Tumour Cells Induced by Sendai Virus

Cited by 22 publications

References 15 publications

Effect of rat andβ-human interferons on hyperacute experimental allergic encephalomyelitis in rats

Effect of rat andβ-human interferons on hyperacute experimental allergic encephalomyelitis in rats

Factors Involved in Interferon-induced or Cholera Toxin-induced Steroidogenesis in Y-1 Mouse Adrenal Tumour Cells

Sarcolectin and interferon in the regulation of cell growth

Contact Info

Product

Resources

About