Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith

Influenza DNA vaccine purification using pHEMA cryogel support

“…The results indicate that the pHEMA cryogel is selective for sc pDNA and allows its recovery with a high purity and yields [24,30]. The purity level of sc NTC 7482-41H-VA2 HA plasmid described for agmatine monolith with an increased stepwise of NaCl was 98.3%, while the recovery yield was 45.3% after two chromatographic runs [13]. Similar results were described for ethylenediamine monolith also using an increased gradient of NaCl, with a purity level and recovery yield of 97.1% and 47%, respectively [12].…”

“…The use of an increased stepwise gradient of NaCl in purification method has shown tremendous advantages over (NH 4 ) 2 SO 4 , such as cost reduction and less environmental impact [23]. Moreover, the use of NaCl in mobile phase was successful applied in the purification of sc pDNA with agmatine monolith [13],…”

“…Dynamic binding capacity (DBC) of a stationary phase is an important parameter in pDNA purification, as showed in previous studies [12][13][14]. The method consists in measure the amount of pDNA that will bind to the column under certain experimental conditions, before occurrence of a break in breakthrough curve [33].…”

“…Recently, several chromatography supports based on ethylenediamine and agmatine monoliths were used to purify a HA influenza deoxyribonucleic acid-based vaccine [12,13]. These monoliths have several advantages over conventional stationary phases, since they present excellent mass transfer properties and flow independence resolution [14].…”

Guanidine improves DEAE anion exchange‐based analytical separation of plasmid DNA

“…Despite their simple structure, both exchangers are partially multimodal and each represents a different combination of characteristics. These differences create opportunities to refine their selectivity with buffers that differentially exploit the individual chemical mechanisms that define their multimodal identities [15].…”