Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system

Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

“…19 Lipase obtained from the fermented medium was purified using ammonium sulphate, membrane dialyse 25 cut-off 10,000-12,000 Da), and an aqueous two-phase system containing a polymer (PEG-8000), phosphate buffer and NaCl, with nominal lipase activity of 906.67 U g -1 , as described by Barbosa et al. 20 For sol gel encapsulation, the silane precursor was tetraethoxysilane, supplied by Acros Organic (NJ, USA), used without further purification. Ethanol (minimum 99%), ammonia (minimum 28%), hydrochloric acid (minimum 36%) and gum arabic were obtained from Synth (São Paulo, Brazil).…”

“…Purification of the enzyme was performed as described by Barbosa et al, 20 entailing stepwise precipitation with ammonium sulfate up to 80% saturation, in an aqueous two-phase system consisting of polyethylene glycol and potassium phosphate salt. Studies have shown that after the purification step, the enzyme is present at a purification factor of about 210 fold.…”

“…ITP-001, isolated from petroleum-contaminated soil in Brazil but to date, immobilization of this enzyme using a sol-gel technique has not been studied for this lipase. [18][19][20] The importance of immobilized lipases can be inferred from the large number of recently published articles related to future industrial applications of lipase-catalyzed reactions. The effects of various factors on the activities and stabilities of lipases need to be studied.…”

Application of an Aqueous Two-Phase Technique for Extraction of Papain from Carica Papaya Latex

“…The results seem to suggest that 5% NaCl is the optimal salt concentration to enhance both purification and yield of papain while 0% gives the lowest results. At 0% salt concentration, the aqueous two phase system does not have enough ionic strength to enhance isolation of the hydrophobic protein as observed also by [12], while at 8.5%, the salt is too much and causes precipitation of the target protein causing lower yields, as also reported by [13]. Fig.2.…”

Influencia Do Uso De Líquidos Iônicos No Processo De Imobilização De Lipase Bacillus Sp. Em Biopolímero

“…A fase de fundo do SAB, que continha a enzima foi dialisada e liofilizada à -50°C. Após esta etapa a enzima foi mantida em frasco fechado e mantida à 4°C (Barbosa et al, 2011). …”