Purification of Phenylalkanoids and Monoterpene Glycosides from <i>Rhodiola rosea</i> L. Roots by High‐speed Counter‐current Chromatography

Mudge, Elizabeth; Lopes‐Lutz, Daise; Brown, Paula N.; Schieber, Andreas

doi:10.1002/pca.2391

Cited by 23 publications

(15 citation statements)

References 34 publications

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“…As previously described, the total amount of rosavins is a more suitable quality control measurement for actual R. rosea content than any single constituents (Brown et al 2002;Ma et al 2011;Mudge et al 2013). In this study, Nuodikang and Rosenrot Forte deviated clearly from the other products in terms of amount of total rosavins.…”

Section: Discussionmentioning

confidence: 53%

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

Thu

Nilsen

Hellum

2016

Pharmaceutical Biology

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Context: Rhodiola rosea L. (Crassulaceae) products are popular natural remedies with a worldwide distribution. Recent studies have revealed potent CYP inhibition by R. rosea extracts both in vitro and in vivo, but information on in vitro CYP inhibition by commercial products are lacking. Variations in commercial R. rosea product quality have also been published. Objective: This study evaluates the variation of in vitro CYP inhibition potential and product quality of six commercially available R. rosea products. Materials and methods: Human CYPs isolated from baculovirus-infected cell system were incubated with testosterone (CYP3A4), dextromethorphan (CYP2D6) or phenacetin (CYP1A2). Positive CYP inhibitors ketoconazole (CYP3A4), quinidine (CYP2D6) and b-naphtoflavone (CYP1A2) were used as controls. Quantification of rosavin, rosarin, rosin, tyrosol and salidroside were used to evaluate R. rosea content. Results: IC 50 values ranged from 7.2-106.6 lg/mL for CYP3A4, 13.0-186.1 lg/mL for 2D6 and 10.7-116.0 lg/mL for 1A2. The tincture formulation of R. rosea was the strongest inhibitor giving the lowest IC 50 values of 7.2 ± 0.7, 13 ± 1.7 and 10.7 ± 5.6 lg/mL, respectively. CYP3A4 was significantly more inhibited by the different products than CYP1A2 (p < .05). One of the six products did not contain any rosavin, rosarin or rosin and is not a R. rosea product. Constituent concentrations were not linked to enzyme inhibition. Discussion and conclusion: The present results show a large variation in inhibitory potential between the products. Several of the products demonstrate similar inhibition levels as the product Arctic Root already proven to inhibit CYP enzyme activity in man.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 53%

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

Thu

Nilsen

Hellum

2016

Pharmaceutical Biology

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“…The metabolites detected were elucidated by the analyses of the 1 H-NMR spectra as well as the comparison with the reference compounds, together with the in-house NMR chemical shift database (Mudge et al, 2013; Luo et al, 2015). The summary of the assignment of 1 H-NMR spectral peaks obtained from the R. rosea, R. crenulata , and R. sachalinensis BRM extracts are provided in Supplement (S3).…”

Section: Resultsmentioning

confidence: 99%

From Traditional Resource to Global Commodities:—A Comparison of Rhodiola Species Using NMR Spectroscopy—Metabolomics and HPTLC

et al. 2016

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The fast developing international trade of products based on traditional knowledge and their value chains has become an important aspect of the ethnopharmacological debate. The structure and diversity of value chains and their impact on the phytochemical composition of herbal medicinal products, as well as the underlying government policies and regulations, have been overlooked in the debate about quality problems in transnational trade. Rhodiola species, including Rhodiola rosea L. and Rhodiola crenulata (Hook. f. & Thomson) H. Ohba, are used as traditional herbal medicines. Faced with resource depletion and environment destruction, R. rosea and R. crenulata are becoming endangered, making them more economically valuable to collectors and middlemen, and also increasing the risk of adulteration and low quality. Rhodiola products have been subject to adulteration and we recently assessed 39 commercial products for their composition and quality. However, the range of Rhodiola species potentially implicated has not been assessed. Also, the ability of selected analytical techniques in differentiating these species is not known yet. Using a strategy previously developed by our group, we compare the phytochemical differences among Rhodiola raw materials available on the market to provide a practical method for the identification of different Rhodiola species from Europe and Asia and the detection of potential adulterants. Nuclear magnetic resonance spectroscopy coupled with multivariate analysis software and high performance thin layer chromatography techniques were used to analyse the samples. Rosavin and rosarin were mainly present in R. rosea but also in Rosea sachalinensis Borris. 30% of the Rhodiola samples purchased from the Chinese market were adulterated by other Rhodiola spp. The utilization of a combined platform based on 1H-NMR and HPTLC methods resulted in an integrated analysis of different Rhodiola species. We identified adulteration at the earliest stage of the value chains, i.e., during collection as a key problem involving several species. This project also highlights the need to further study the links between producers and consumers in national and trans-national trade.

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“…The structures of benzyl and phenol derivatives are shown in Figure . Benzyl β‐ d ‐glucopyranoside (Compound 36 ) is found in R. crenulata , R. rosea , and R. sachalinensis . Two other benzene methane derivatives, phenylmethyl 6‐ O ‐α‐ l ‐arabinopyranosyl‐β‐ d ‐glucopyranoside (Compound 41 ) and phenylmethyl 6‐ O ‐α‐ l ‐arabinofuranosyl‐β‐ d ‐glucopyranoside (Compound 42 ), are isolated from both R. rosea and R. sachalinensis .…”

Section: Chemistry and Quality Controlmentioning

confidence: 99%

Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study

Tao

Cao

et al. 2019

Medicinal Research Reviews

113

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Rhodiola species, belonging to the family Crassulaceae, have long been used as an adaptogen, tonic, antidepressant, and antistress medicine or functional food in Asia and Europe. Due to the valuable application, the growing demand of Rhodiola species has led to a rapid decrease in resource content. This review aims to summarize the integrated research progress of seven mainstream Rhodiola species. We first outline both traditional and current use of Rhodiola for the treatment of various diseases. A detailed summary and comparison of chemical, pharmacological, toxicological, and clinical studies of various Rhodiola species highlight recent scientific advances and gaps, which gives insights into the understanding of Rhodiola application and would be helpful to improve the situation of biological resources and diversities of Rhodiola plants.

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Purification of Phenylalkanoids and Monoterpene Glycosides from Rhodiola rosea L. Roots by High‐speed Counter‐current Chromatography

Abstract: High-speed counter-current chromatography was successful in the isolation of several phytochemicals present in Rhodiola rosea roots, including two components that are not commercially available.

Cited by 23 publications

References 34 publications

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

From Traditional Resource to Global Commodities:—A Comparison of Rhodiola Species Using NMR Spectroscopy—Metabolomics and HPTLC

Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study

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