Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye

Guo, Mei; Lu, Fuping; Liu, Minyao; Li, Tuoping; Pu, Jun; Wang, Na; Peng, Liang; Zhang, Chenyun

doi:10.1007/s10529-008-9817-z

Cited by 35 publications

(14 citation statements)

References 14 publications

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“…These results are in agreement with previously reported purified fungal laccases, which showed extremely high activity in the decolorization of anthraquinone-like dyes [38,39], but reduced activity against azo-like dyes [40]. This finding may be attributed to the different structural features of the dyes, i.e., unlike azo dyes, anthraquinone dyes are direct substrates of laccase-based oxidation.…”

Section: Discussionsupporting

confidence: 92%

Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase

et al. 2012

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BackgroundMicrobial laccases are highly useful in textile effluent dye biodegradation. However, the bioavailability of cellularly expressed or purified laccases in continuous operations is usually limited by mass transfer impediment or enzyme regeneration difficulty. Therefore, this study develops a regenerable bacterial surface-displaying system for industrial synthetic dye decolorization, and evaluates its effects on independent and continuous operations.ResultsA bacterial laccase (WlacD) was engineered onto the cell surface of the solvent-tolerant bacterium Pseudomonas putida to construct a whole-cell biocatalyst. Ice nucleation protein (InaQ) anchor was employed, and the ability of 1 to 3 tandemly aligned N-terminal repeats to direct WlacD display were compared. Immobilized WlacD was determined to be surface-displayed in functional form using Western blot analysis, immunofluorescence microscopy, flow cytometry, and whole-cell enzymatic activity assay. Engineered P. putida cells were then applied to decolorize the anthraquinone dye Acid Green (AG) 25 and diazo-dye Acid Red (AR) 18. The results showed that decolorization of both dyes is Cu2+- and mediator-independent, with an optimum temperature of 35°C and pH of 3.0, and can be stably performed across a temperature range of 15°C to 45°C. A high activity toward AG25 (1 g/l) with relative decolorization values of 91.2% (3 h) and 97.1% (18 h), as well as high activity to AR18 (1 g/l) by 80.5% (3 h) and 89.0% (18 h), was recorded. The engineered system exhibited a comparably high activity compared with those of separate dyes in a continuous three-round shake-flask decolorization of AG25/AR18 mixed dye (each 1 g/l). No significant decline in decolorization efficacy was noted during first two-rounds but reaction equilibriums were elongated, and the residual laccase activity eventually decreased to low levels. However, the decolorizing capacity of the system was easily retrieved via a subsequent 4-h cell culturing.ConclusionsThis study demonstrates, for the first time, the methodology by which the engineered P. putida with surface-immobilized laccase was successfully used as regenerable biocatalyst for biodegrading synthetic dyes, thereby opening new perspectives in the use of biocatalysis in industrial dye biotreatment.

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Section: Discussionsupporting

confidence: 92%

Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase

et al. 2012

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“…Of particular interest, SMC decolorized the diazo-like dye Congo red without any mediator, even though this dye is not a common substrate for the laccase catalytic reaction [6]. These results are in agreement with those previously reported on purified fungal laccases, which showed extremely high activity in decolorizing anthraquinone-like dyes [19] but reduced activity against azo-like dyes [20]. Before measurements, the full wavelength absorption spectra (from 400 nm to 800 nm) of the dyes were recorded, which gave the maximal spectral peak at 595 nm for RBBR (Fig.…”

Section: Resultssupporting

confidence: 86%

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

2013

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This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at 4℃ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.

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“…Bioaccumulation has also been applied to the decontamination of pollutants, by using microorganisms such as bacteria, algae, yeast, and fungi (Guo et al, 2008;Gonzalez and Fuentes, 2009;Kotrba et al, 2011). The most important advantage of microorganisms is their selectivity in the uptake of pollutants.…”

Section: Introductionmentioning

confidence: 99%

Decolorization of hair dye by lignocellulosic waste materials from contaminated waters

Zahir

Naidoo

Kostadinova

et al. 2014

Front. Environ. Sci.

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Basic yellow 57 (BY57) was chosen as a model hair dye due to its prevalence in cosmetics wastewaters. This study proposes the use of lignocellulosic materials like spent tea leaves of peppermint (PM), chai tea (CT), and chamomile (CM) as raw adsorbents for the removal of BY57 from contaminated solutions. Batch adsorption experiments were carried out at room temperature to achieve the maximum adsorption capacity. Results indicate that the highest removal is achieved at pH 6-8, with a minimum adsorbent mass of 75 mg and in the absence of salinity, crowding agents and heavy metals. Adsorption equilibria were modeled according to the Langmuir and Freundlich isotherm theories and reported the following trend: PM > CT > CM, reaching q max values of 105, 80, and 38 mg of dye per gram of adsorbent, respectively. Desorption experiments showed that diluted solution of HCl is able to desorb up to 80% of the dye and recover the adsorbent to be used in consecutive cycles. Finally, the adsorbents were characterized by Scanning electron microscopy (SEM), indicating that the adsorbents have a porous and heterogeneous surface, showing pockets and protrusions that are potential adsorption sites for the dye.

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Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye

Cited by 35 publications

References 14 publications

Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase

Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

Decolorization of hair dye by lignocellulosic waste materials from contaminated waters

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