Purification of the mitosis-stimulating factor from Phaseolus vulgaris

Börjeson, Jan; Bouveng, R.; Gardell, S.; Nordén, Å.; Thunell, Stig

doi:10.1016/0304-4165(64)90020-0

Cited by 41 publications

(12 citation statements)

References 5 publications

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“…In order to isolate from the extract the substances that stimulate RNA synthesis responsible for the effects shown in Fig. 1, the following purification procedure was used: Red kidney beans (Phaseolus vulgaris) were extracted for preparation of "dry powder" (15). Fraction I (F I) was prepared by extraction of 6.0 g of "dry powder" (derived from 500 g of beans) with 100 ml of PM buffer (8).…”

Section: Methodsmentioning

confidence: 99%

Isolation of Substances That Stimulate RNA Synthesis from Red Kidney Bean: Their Activities in Lymphocytes and Escherichia coli

Harms‐Ringdahl

Fedorcsák

Ehrenberg

1973

Proc. Natl. Acad. Sci. U.S.A.

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An extract from red kidney beans (Phaseolus vulgaris) was fractionated with respect to RNA synthesis-stimulating activity. The activity of the fractions was tested in chicken-spleen lymphocytes cultured in serum-free medium and in plasmolyzed E. coli cells. Lymphocyte-stimulating activity was found in a fraction called F III. This fraction contained protein and orcinolpositive carbohydrate. The protein (called F III p) responsible for the observed activity was separated from the contaminating carbohydrate by acid precipitation. F III p appears to belong to the group of well-known mitogens of high molecular weight present in red kidney beans. A bacteria-stimulating factor was found in a fraction called F V. This factor strongly stimulated RNA synthesis in the bacterial system, and had a weak stimulating effect in the lymphocyte system. In plasmolyzed E. coli cells, F V counteracted the inhibiting effect of rifampicin on RNA synthesis.

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Section: Methodsmentioning

confidence: 99%

Isolation of Substances That Stimulate RNA Synthesis from Red Kidney Bean: Their Activities in Lymphocytes and Escherichia coli

Harms‐Ringdahl

Fedorcsák

Ehrenberg

1973

Proc. Natl. Acad. Sci. U.S.A.

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“…[11], contained 2.8 mg protein per ml, and was stored frozen at -20°. A new vial was opened for each experiment.…”

Section: Methodsmentioning

confidence: 99%

The Mechanism of Glucocorticoid Action on the Phytohemagglutinin-Stimulated Lymphocyte

Eurenius¹,

McIntyre²

1970

Int Arch Allergy Immunol

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“…The mitosis-stimulating factor of red kidney beans (Phaseolus vulgaris) has been purified by Börjeson et al [7]. The method con sists essentially in removing inert protein by heat dénaturation, followed by ethanol fractionation of the soluble extract and chromato graphy on calcium phosphate gel.…”

Section: Hemagglutinins From Leguminosaementioning

confidence: 99%

Phytohemagglutinine - A Survey of Recent Progress

Dechary¹

1968

Vox Sanguinis

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This paper is a review of recent articles by other investigators on phytohemagglutinins and is by necessity selective in nature. Previously known hemagglutinins of members of the family Leguminosae (Phaseolus, Soja) have been purified and some of their physical and chemical properties determined. The mitosis-stimulating factor of P. vulgaris has been purified and the hemagglutinating component separated from it. Another member of Leguminosae (Wistaria) has been found to possess mitogenic and blastogénie properties and the pokeweed mitogen has been isolated and characterized. Concanavalin A, the nonspecific hemagglutinin of jack bean, has been highly purified and its quaternary structure determined. The determination of stereochemical requirements for inhibition studies with hemagglutinins now provides a new tool for the solution of unresolved problems in carbohydrate identification and structure. Toxic proteins have been isolated from several members of the family Euphorbiacae. Ricin B, the toxic, nonhemagglutinating protein of the castor bean has been highly purified and some of its physical properties determined.

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Purification of the mitosis-stimulating factor from Phaseolus vulgaris

Cited by 41 publications

References 5 publications

Isolation of Substances That Stimulate RNA Synthesis from Red Kidney Bean: Their Activities in Lymphocytes and Escherichia coli

Isolation of Substances That Stimulate RNA Synthesis from Red Kidney Bean: Their Activities in Lymphocytes and Escherichia coli

The Mechanism of Glucocorticoid Action on the Phytohemagglutinin-Stimulated Lymphocyte

Phytohemagglutinine - A Survey of Recent Progress

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