Purifying selection against gene conversions between the polyamine transport (TPO) genes of Saccharomyces species

Sampathkumar, Gowthami; Drouin, Guy

doi:10.1007/s00294-014-0445-y

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Lucklessly, the transcription profile of genes in polyamine biosynthesis like SPE1, SPE2, SPE3 and SPE4 was not obtained in this study, except for up-regulations of polyamine transporter-encoding genes including TPO2, TPO3 and TPO4 (Table 4 and Fig. 4), which are important for polyamines uptake and excretion (78,79). Also, SAM is a precursor of GSH (80), which play a major role in GRX system for removal of ROS-mediated damages and xenobiotics (25).…”

Section: Heat Shock Proteins (Hsps) Chaperone Stress Conditions Like contrasting

confidence: 57%

Negative effects characterization and comparative transcriptomics elucidation on the lag phase of an industrialS. cerevisiaeunder the corn stover hydrolysate stress

Kuang

Guo

Zhang

et al. 2020

Preprint

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During biofuels fermentation from pretreated lignocellulosic biomass, the strong toxicity of the lignocellulose hydrolysate is resulted from the synergistic effect of multiple lignocellulosic inhibitors, which far exceeds the sum of effects caused by every single inhibitor. Meanwhile, the synergistic effect is unclear and the underlying response mechanism of the industrial yeast towards the actual pretreated lignocellulose hydrolysate is still under exploration. Here, we employed an industrial S. cerevisiae for the transcriptomic analysis in two time points (early and late) of the lag phase under the corn stover hydrolysate stress. As investigation, the corn stover hydrolysate caused the accumulation of reactive oxygen species (ROS), damages of mitochondrial membrane and endoplasmic reticulum (ER) membrane in the industrial S. cerevisiae YBA_08 during the lag phase, especially these negative effects were more significant at the early lag phase. Based on the transcriptome profile, the industrial S. cerevisiae YBA_08 might recruit stress-related transcription factors (MSN4, STE12, SFL1, CIN5, COM2, MIG3, etc.) through the mitogen-activated protein kinase (MAPK)-signaling pathway to induce a transient G1/G2 arrest, and to activate defense bioprocesses like protectants metabolism, sulfur metabolism, glutaredoxin system, thioredoxin system, heat shock proteins chaperone and oxidoreductase detoxification, resisting those compounded stresses including oxidative stress, osmotic stress and structural stress. Surprisingly, this defense system might be accompanied with the transient repression of several bioprocesses like fatty acid metabolism, purine de novo biosynthesis and ergosterol biosynthesis.Importance This research systematically demonstrated the lag phase response of an industrial yeast to the lignocellulosic hydrolysate in transcriptional level, providing a molecular fundament for understanding the synergistic effect of various lignocellulosic inhibitors and the regulatory mechanism of tolerance for industrial yeasts under this stress.

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Section: Heat Shock Proteins (Hsps) Chaperone Stress Conditions Like contrasting

confidence: 57%

Negative effects characterization and comparative transcriptomics elucidation on the lag phase of an industrialS. cerevisiaeunder the corn stover hydrolysate stress

Kuang

Guo

Zhang

et al. 2020

Preprint

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“…Natural populations of yeasts display considerable levels of genetic and phenotypic diversity, in part allowing them to conquer a wide range of ecological and geographical habitats (Liti et al 2009, Schacherer et al 2009, Liti and Schacherer 2011, Skelly et al 2013, Almeida et al 2014, Hirakawa et al 2014, Fawcett et al 2014, Sampathkumar and Drouin 2015, Ford et al 2015, Jeffares et al 2015). Nevertheless, as demonstrated by recent studies (Charron, et al 2014, Hou, et al 2014, Hou, et al 2015), independently segregating genetic variants in diverging populations could occasionally cause them to be reproductively isolated, leading to reduced offspring viability upon hybridization.…”

Section: Main Textmentioning

confidence: 99%

Negative epistasis: a route to intraspecific reproductive isolation in yeast?

Hou

Schacherer

2015

Curr Genet

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Summary Exploring the molecular bases of intraspecific reproductive isolation captures the ongoing phenotypic consequences of genetic divergence and provides insights into the early onset of speciation. Recent species-wide surveys using natural populations of yeasts demonstrated that intrinsic post-zygotic reproductive isolation segregates readily within the same species, and revealed the multiplicity of the genetic mechanisms underlying such processes. These advances deepened our current understandings and opened further perspectives regarding the complete picture of molecular and evolutionary origins driving the onset intraspecific reproductive isolation in yeasts.

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“…The knowledge about polyamine exporters in plants is lacking, while PotE and CadB have been reported as PA antiporters in E. coli (Schiller et al, 2000;Igarashi and Kashiwagi, 2010). Similarly TPO1-5 were characterized as PA excretion proteins in yeast (Aouida et al, 2005;Uemura et al, 2005;Uemura et al, 2007;Sampathkumar and Drouin, 2015). Based on the homology to these antiporters, bi-directional amino acid transporters (BATs) were identified as PA antiporters in Arabidopsis and rice (Ge, 2015;Ariyaratne et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic portrait of polyamine transporters in barley: insights in the regulation of leaf senescence

et al. 2023

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Nitrogen (N) is one of the most expensive nutrients to supply, therefore, improving the efficiency of N use is essential to reduce the cost of commercial fertilization in plant production. Since cells cannot store reduced N as NH3 or NH4+, polyamines (PAs), the low molecular weight aliphatic nitrogenous bases, are important N storage compounds in plants. Manipulating polyamines may provide a method to increase nitrogen remobilization efficiency. Homeostasis of PAs is maintained by intricate multiple feedback mechanisms at the level of biosynthesis, catabolism, efflux, and uptake. The molecular characterization of the PA uptake transporter (PUT) in most crop plants remains largely unknown, and knowledge of polyamine exporters in plants is lacking. Bi-directional amino acid transporters (BATs) have been recently suggested as possible PAs exporters for Arabidopsis and rice, however, detailed characterization of these genes in crops is missing. This report describes the first systematic study to comprehensively analyze PA transporters in barley (Hordeum vulgare, Hv), specifically the PUT and BAT gene families. Here, seven PUTs (HvPUT1-7) and six BATs (HvBAT1-6) genes were identified as PA transporters in the barley genome and the detailed characterization of these HvPUT and HvBAT genes and proteins is provided. Homology modeling of all studied PA transporters provided 3D structures prediction of the proteins of interest with high accuracy. Moreover, molecular docking studies provided insights into the PA-binding pockets of HvPUTs and HvBATs facilitating improved understanding of the mechanisms and interactions involved in HvPUT/HvBAT-mediated transport of PAs. We also examined the physiochemical characteristics of PA transporters and discuss the function of PA transporters in barley development, and how they help barley respond to stress, with a particular emphasis on leaf senescence. Insights gained here could lead to improved barley production via modulation of polyamine homeostasis.

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Purifying selection against gene conversions between the polyamine transport (TPO) genes of Saccharomyces species

Cited by 5 publications

References 26 publications

Negative effects characterization and comparative transcriptomics elucidation on the lag phase of an industrialS. cerevisiaeunder the corn stover hydrolysate stress

Negative effects characterization and comparative transcriptomics elucidation on the lag phase of an industrialS. cerevisiaeunder the corn stover hydrolysate stress

Negative epistasis: a route to intraspecific reproductive isolation in yeast?

Genetic portrait of polyamine transporters in barley: insights in the regulation of leaf senescence

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