Purple-bacterial photosynthetic reaction centers and quantum‐dot hybrid‐assemblies in lecithin liposomes and thin films

Lukashev, E. P.; Knox, P. P.; Gorokhov, V. V.; Grishanova, N. P.; Seifullina, N. Kh.; Krikunova, Maria; Lokstein, Heiko; Paschenko, V.Z.

doi:10.1016/j.jphotobiol.2016.09.009

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…This was first demonstrated using chromophores which were covalently attached to LH membrane proteins isolated in detergents, including synthetic organic compounds [11][12][13][14] or quantum dots. 15,16 FRET has also been demonstrated between noncovalently coupled chromophores incorporated into lipid bilayers [17][18][19] or polymer micelles. [20][21][22] LHCII has previously been integrated into model membranes, e.g., lipid vesicles [23][24][25][26][27][28] or lipid nanodiscs, 28,29 and these have been studied to understand its biophysical properties, but not to enhance its spectral range.…”

Section: Main Textmentioning

confidence: 99%

Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores

Hancock

Meredith

Connell

et al. 2019

Preprint

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Biology provides a suite of optically-active nanomaterials in the form of "light harvesting" protein-chlorophyll complexes, however, these have drawbacks including their limited spectral range. We report the generation of model lipid membranes (proteoliposomes) incorporating the photosynthetic protein Light-Harvesting Complex II (LHCII) and lipid-tethered Texas Red (TR) chromophores that act as a "bio-hybrid" energy transferring nanomaterial. The effective spectral range of the protein is enhanced due to highly efficient energy transfer from the TR chromophores (up to 94%), producing a marked increase in LHCII fluorescence (up to 3x).Our self-assembly procedure offers excellent modularity allowing the incorporation of a range of concentrations of energy donors (TR) and acceptors (LHCII), allowing the energy transfer efficiency (ETE) and LHCII fluorescence to be tuned as desired. Fluorescence Lifetime Imaging Microscopy (FLIM) provides single-proteoliposome-level quantification of ETE, revealing distributions within the population and proving that functionality is maintained on a surface. Our membrane-based system acts as a controllable light harvesting nanomaterial with potential applications as thin films in photo-active devices.

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Section: Main Textmentioning

confidence: 99%

Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores

Hancock

Meredith

Connell

et al. 2019

Preprint

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“…Одним из важных моментов является стабильность функционирования подобных устройств в разных условиях. Она может достигаться в том числе высушиванием сохраняющих активность гибридных конструкций, а также включением их в различные органические матрицы [1,2]. Важнейшим фактором, влияющим на перенос энергии электронного возбуждения от КТ к РЦ, является температура.…”

Section: биохимия биофизика молекулярная биологияunclassified

“…Известно, что КТ -высокостабильные структуры, а РЦ сохраняют максимальную (100%) квантовую эффективность разделения зарядов при варьировании температуры от комнатных до криогенных значений [3]. 1 Предметом настоящей работы явилось исследование эффективности миграции энергии от КТ к РЦ в широком диапазоне температур.…”

Section: биохимия биофизика молекулярная биологияunclassified

“…Из характера кривых на рис. 1 ∝ ⋅ ϕ χ [9]. Кроме этого, величина интеграла перекрывания спектров зависит от молярного коэффициента экстинкции ε, величина которого также может изменяться с температурой.…”

Section: биохимия биофизика молекулярная биологияunclassified

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Intramolecular mobility affects the energy migration from quantum dots to reaction centers of photosynthesizing bacterium rb. Sphaeroides

Krasilnikov

Лукашев

Knox

et al. 2019

Доклады Академии наук

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The temperature dependence of the efficiency of energy migration from the CdSe/CdS/ZnS quantum dots (QDs, a fluorescence maximum at 580 nm) to the reaction centers (RCs) of the bacteria Rb.sphaeroides is practically constant over the temperature range from 100 to ~230–240 K but then decreases 2,5–3 times as temperature further increases to 310 K. The analysis of this dependence on the basis of Forster’s theory showed that the major changes in the energy transfer efficiency are associated with the temperature change in the quantum yield of QD fluorescence, which is due to the activation of intramolecular mobility in the RC structure.

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“…The other solution is to use quantum dots (QDs), which absorb ultraviolet and longwavelength light much more efficiently than natural lightharvesting proteins and transfer the excitation energy to photosynthetic RCs. Dry films of bacterial RCs and CdSe/ ZnS QDs, maintained at atmospheric humidity, are capable of maintaining their functional activity for at least some months according to measurements of their spectral characteristics, efficiency of energy transfer from QDs to RCs, and RC electron transport activity [89][90][91].…”

Section: Redox Equivalentsmentioning

confidence: 99%

Functional Nanohybrid Materials from Photosynthetic Reaction Center Proteins

Hajdu

Szabó

Sarrai

et al. 2017

International Journal of Photoenergy

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Application of technical developments in biology and vice versa or biological samples in technology led to the development of new types of functional, so-called "biohybrid" materials. These types of materials can be created at any level of the biological organization from molecules through tissues and organs to the individuals. Macromolecules and/or molecular complexes, membranes in biology, are inherently good representatives of nanosystems since they fall in the range usually called "nano." Nanohybrid materials provide the possibility to create functional bionanohybrid complexes which also led to new discipline called "nanobionics" in the literature and are considered as materials for the future. In this publication, the special characteristics of photosynthetic reaction center proteins, which are "nature's solar batteries," will be discussed in terms of their possible applications for creating functional molecular biohybrid materials.

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Purple-bacterial photosynthetic reaction centers and quantum‐dot hybrid‐assemblies in lecithin liposomes and thin films

Cited by 17 publications

References 43 publications

Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores

Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores

Intramolecular mobility affects the energy migration from quantum dots to reaction centers of photosynthesizing bacterium rb. Sphaeroides

Functional Nanohybrid Materials from Photosynthetic Reaction Center Proteins

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