Purple grape juices prevent pentylenetetrazol-induced oxidative damage in the liver and serum of Wistar rats

Abstract

Oxidative damages in hepatocytes may be caused by epilepsy and/or anticonvulsant drugs. Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen species. Organic and conventional grape juices are rich in polyphenols, compounds with important antioxidant activity. It is hypothesized that organic and conventional purple grape juices may have protective effect against oxidative damage induced by pentylenetetrazole (PTZ) (a standard convulsant drug) in the liver and serum of Wistar rats. Animals (n = 16 in each group) received, by gavage, saline, organic grape juice or conventional grape juice (10 μL/g of body weight) for 17 days. Subsequently, half of the rats in each group received PTZ (60 mg/kg). After 30 minutes, the animals were euthanized by decapitation. Liver and blood samples were isolated to evaluate oxidative parameters (lipid and protein oxidation, nitric oxide metabolite content, antioxidant defenses, and protein sulfhydryl content). The results of this study showed that although organic juice contains higher polyphenol content than conventional juice, both juices conferred protection against lipid and protein oxidative damage and limited the increase in PTZ-induced nitric oxide metabolite content in the liver and serum. In addition, both juices inhibited the PTZ-induced reduction in enzymatic antioxidant defenses (superoxide dismutase and catalase activities) and sulfhydryl protein content in the liver and serum. In summary, both organic and conventional grape juices were able to reduce oxidative damage induced by PTZ in the liver and serum of Wistar rats.

Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: Method validation and characterization of São Francisco Valley samples

“…The anthocyanins were the main phenolic class detected in V. labrusca, hybrid, Tempranillo and Alicante Bouschet grape juices, corresponding to 44.35 to 70.33% of the total phenolic content quantified for the samples. These results were consistent with those described by Rodrigues et al [32] and Stalmach et al [33]. It is known that the anthocyanins are responsible for the coloration of the grape, have the ability to scavenge the excess radicals and may play a role in the prevention of diseases [34][35][36].…”

Effect of grape juice consumption on antioxidant activity and interleukin-6 concentration in lactating rats

“…Trolox/g) lower than that found in the present study. While Rodrigues et al (2013) (21), using the method of reactive substances to the thiobarbituric acid (TBA), using adults rats, divided into 3 groups (control, organic grape juice and conventional grape juice) noted that although the organic juice has higher polyphenol content than the conventional grape juice, both were able to reduce the oxidative damage induced by (pentylenetetrazole) PTZ in plasm of the Wistar rats.…”

Differential effects of grape juice on gastric emptying and renal function from cisplatin-induced acute adverse toxicity

“…Whole grape juice was administered for 6 days before the induction of cisplatin and then continued for two additional days. In the present study, whole grape juices were administered according to the method proposed by Rodrigues et al 15 The doses of grape juice were determined by calculating the average amount of juice that would be equally consumed daily by a 70 kg human male. 21 Hence, whole grape juices were administered to the rats at 10 mL/g of body weight once per day.…”

“…[12][13][14] Consumption of grape juice at a concentration of 10 mL/g body weight in Wistar rats with pentylenetetrazol-induced oxidative damage for 17 days resulted in an improvement in liver and serum oxidative damage by increased enzymatic antioxidant defenses. 15 In addition, grape juice concentrate has been found to protect against oxidative DNA damage induced by hydrogen peroxide (H 2 O 2 ) in peripheral blood cells in rats. 16 Clinical studies have reported that concentrated red grape juice supplementation exerts antioxidant, hypolipidemic, and anti-inflammatory effects in both healthy subjects and hemodialysis patients.…”