Purple sweet potato extract maintains intestinal homeostasis and extend lifespan through increasing autophagy in female Drosophila melanogaster

Abstract

Purple sweet potatoes extract (PSPE) have been used as a natural food antioxidant with high anthocyanin concentrations. Research investigated the lifespan and the mechanisms of PSPE on female Drosophila melanogaster. Supplementation of PSPE extended the lifespan by 16.3% and had a protective effect on injury by oxidative stress. PSPE treatment enhanced the endogenous antioxidant enzyme activity and reduced malondialdehyde (MDA) content. Furthermore, PSPE significantly up‐regulated foxo‐related genes, inhibited mTOR mRNA expression, and activated autophagy to maintain intestinal homeostasis. Meanwhile, PSPE improved intestinal barrier dysfunction by 22.86%, decelerated the abnormal proliferation rate of intestinal stem cell (ISCs) by 23.77%, and improved intestinal integrity in geriatric D. melanogaster. In conclusion, PSPE may maintain intestinal homeostasis, and improve the antioxidant and stress resistance capacity through the insulin and rapamycin pathway, thereby extending the lifespan. Therefore, it provides active support to the development and application of PSPE in functional food. Practical applications In recent years, with the increase of age, age‐related complications have generally increased and seriously affected people’s healthy life. Purple sweet potato is a nutrient‐rich substance, which not only has a unique color but also contains rich anthocyanins, so it has many potential biological and pharmacological functions. Our results showed that the PSPE had a good effect of maintaining the intestinal homeostasis of the older adult, and provided a favorable theoretical basis for the development of PSPE functional products and scientific academic research.

Hawthorn extract inhibited the PI3k /Akt pathway to prolong the lifespan of Drosophila melanogaster

“…As shown in Figure 5c,d, the intestinal microflora was significantly reduced after administration of HE, and intestinal homeostasis was maintained. It is in line with Zhang, Wang, et al (2021), whose study reported that purple sweet potatoes extract, with high anthocyanin concentrations, could sustain intestinal homeostasis of D. melanogaster with aging. Moreover, aging and tissue damage led to abnormal proliferation and enhance terminal differentiation of ISCs.…”

“…The method of lifespan determination is based on the literature (Zhang, Wang, et al, 2021). Two‐day‐old D. melanogaster was divided into 4 groups, with 10 bottles in each group and 20 flies in each bottle.…”

Naringin ameliorates H 2O 2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway

“…Food intake was determined by referring to the method of previous studies [14] . Briefly, wild-type flies (n = 100 per group) were cultured for 4 days in diets with or without naringin or resveratrol, then placed in empty tubes for 2 h. The flies were moved to diets including 0.2% rhodamine B sulfonate sodium salt (Sigma, USA) for 3 h. A subjective rating scale ranging from 0 to 5 (colorless abdomen to completely red) was used to reflect the food intake.…”

Anti-Aging Potential of Bioactive Phytoconstituents Found in Edible Medicinal Plants: A Review

“…Due to their known antioxidative potential, the capacity of anthocyanins to delay aging or the progression of age-related diseases has therefore been explored [94,95]. For instance, purple sweet potato extracts, known to contain a high quantity of anthocyanins, have been investigated for their life-prolonging potential in Drosophila melanogaster [96]. In their research, NAME examined the effect of purified purple sweet potato extract on female wild-type D. melanogaster.…”