Purple sweet potato pigments protect murine thymocytes from⁶⁰Co γ-ray-induced mitochondria-mediated apoptosis

Abstract

Pre-treatment with PSP pigments significantly inhibited ⁶⁰Co γ-ray-induced mitochondria-mediated apoptosis. This radioprotective effect might be related to ROS scavenging, the enhancement of the activity of antioxidant enzymes, the maintenance of mitochondrial transmembrane potential, and the sequential inhibition of cytochrome c release and downstream caspase and PARP cleavage.

Purple Sweet Potato Pigments Scavenge ROS, Reduce p53 and Modulate Bcl-2/Bax to Inhibit Irradiation-induced Apoptosis in Murine Thymocytes

“…The cell viability assays showed that 60 Co γ-ray irradiation inhibited the cell survival and PSP pigments enhanced the cell viability in a dose-dependent manner without causing cytotoxicity when applied in their optimum doses. The results were in line with our previous study [15] and further demonstrated the radioprotective effects of PSP pigments. Following exposure to irradiation, a cell-specific program of apoptosis will be engaged.…”

“…There has been limited information about the radioprotective effects of PSP pigments. In our previous study we have found that PSP pigments could inhibit the 60 Co γ-ray-induced apoptosis in murine thymocytes by mitochondrial way [15]. In the present study, we further investigated the effect of PSP pigments on the apoptosis induced by 60 Co γ-ray irradiation and the roles of ROS, p53 and Bcl-2 family members involved in.…”

“…Protein concentration was determined by bicinchonic acid (BCA) method. Equal amounts of total protein was fractionated by SDS-PAGE and preformed as previously described [15]. The band density was analyzed by Quantity One software (Bio-Rad Laboratories, Hercules, CA, USA).…”