Putative link between Staphylococcus aureus bacteriophage serotype and community association

Mohamed, Doumbouya; Saberesheikh, S.; Kearns, Angela; Saunders, Nicholas A.

doi:10.1016/j.ijmm.2012.02.002

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…Several studies have speculated that CC398 MSSA originated in humans but lost human associated factors such as Panton-Valentine Leukocidin (PVL)-associated phages, toxic shock syndrome toxin I and exfoliative toxins, which are markers of community associated (CA)-MRSA and hospital associated (HA)-MRSA strains (Kadlec et al, 2012; Mohamed et al, 2012; Ballhausen et al, 2017), and acquired antibiotic resistance genes such as mecA and tetM as they adapted to livestock (Fitzgerald, 2012a, b; Price et al, 2012). Furthermore, several investigations have shown that persons in contact with livestock may be at increased risk of becoming colonized with LA-MRSA.…”

Section: Introductionmentioning

confidence: 99%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus From Animals and Animal Products in the UK

et al. 2019

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is an emerging problem in many parts of the world. Although animal-adapted LA-MRSA has been known for many years, recent reports suggest a possible increasing trend in the zoonotic transmission of LA-MRSA in Europe. Since its emergence in the early 2000’s, several investigations have indicated that persons in prolonged, repeated contact with affected livestock are at a higher risk of becoming colonized with LA-MRSA. LA-MRSA monitoring in livestock is voluntary under current EU legislation, and not all member states, including the UK, participate. UK LA-MRSA isolates have been detected through scanning surveillance, where samples are submitted from clinically diseased livestock for diagnostic investigation, and research studies. Surveys conducted on retail beef, pig and poultry meat on sale in the UK have also detected LA-MRSA. Taken together these results suggest that LA-MRSA is present in the UK, possibly at low prevalence level, as suggested by available evidence. In this review, we examine the data available from UK livestock and animal products, and make recommendations for future. We also review the findings from whole genome sequencing (WGS) of the possible lineage of some UK livestock isolates.

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Section: Introductionmentioning

confidence: 99%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus From Animals and Animal Products in the UK

et al. 2019

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“…Numerous studies have shown that many MRSA clones that occur sporadically or not at all in one geographic region are often prevalent in another region and vice versa (20,22,23). However, previous studies that have investigated sporadic MRSA populations are limited in terms of sample size and/or depth of analysis (24)(25)(26)(27).…”

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confidence: 99%

Extensive Genetic Diversity Identified among Sporadic Methicillin-Resistant Staphylococcus aureus Isolates Recovered in Irish Hospitals between 2000 and 2012

Kinnevey

Shore

Brennan

et al. 2014

Antimicrob Agents Chemother

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e Clonal replacement of predominant nosocomial methicillin-resistant Staphylococcus aureus (MRSA) strains has occurred several times in Ireland during the last 4 decades. However, little is known about sporadically occurring MRSA in Irish hospitals or in other countries. Eighty-eight representative pvl-negative sporadic MRSA isolates recovered in Irish hospitals between 2000 and 2012 were investigated. These yielded unusual pulsed-field gel electrophoresis and antibiogram-resistogram typing patterns distinct from those of the predominant nosocomial MRSA clone, ST22-MRSA-IV, during the study period. Isolates were characterized by spa typing and DNA microarray profiling for multilocus sequence type (MLST) clonal complex (CC) and/or sequence type (ST) and SCCmec type assignment, as well as for detection of virulence and antimicrobial resistance genes. Conventional PCR-based SCCmec subtyping was undertaken when necessary. Extensive diversity was detected, including 38 spa types, 13 MLST-CCs (including 18 STs among 62 isolates assigned to STs), and 25 SCCmec types (including 2 possible novel SCCmec elements and 7 possible novel SCCmec subtypes). Fifty-four MLST-spa-SCCmec type combinations were identified. Overall, 68.5% of isolates were assigned to nosocomial lineages, with ST8-t190-MRSA-IID/IIE ؎ SCC M1 predominating (17.4%), followed by CC779/ST779-t878-MRSA-SCCmec-SCC-SCC CRISPR (7.6%) and CC22/ST22-t032-MRSA-IVh (5.4%). Community-associated clones, including CC1-t127/t386/t2279-MRSA-IV, CC59-t216-MRSA-V, CC8-t008-MRSA-IVa, and CC5-t002/t242-MRSA-IV/V, and putative animal-associated clones, including CC130-t12399-MRSA-XI, ST8-t064-MRSA-IVa, ST398-t011-MRSA-IVa, and CC6-t701-MRSA-V, were also identified. In total, 53.3% and 47.8% of isolates harbored genes for resistance to two or more classes of antimicrobial agents and two or more mobile genetic element-encoded virulence-associated factors, respectively. Effective ongoing surveillance of sporadic nosocomial MRSA is warranted for early detection of emerging clones and reservoirs of virulence, resistance, and SCCmec genes.

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The chronic wound virome: phage diversity and associations with wounds and healing outcomes

Verbanic

Deacon

Chen

2022

Preprint

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Two leading impediments to chronic wound healing are polymicrobial infection and biofilm formation. Recent studies have characterized the bacterial fraction of these microbiomes and have begun to elucidate compositional correlations to healing outcomes. However, the factors that drive compositional shifts are still being uncovered. The virome may play an important role in shaping bacterial community structure and function. Previous work on the skin virome determined that it was dominated by bacteriophages, viruses that infect bacteria. To characterize the virome, we enrolled 20 chronic wound patients presenting at an outpatient wound care clinic in a microbiome survey, collecting swab samples from healthy skin and chronic wounds before and after a single, sharp debridement procedure. We investigated the virome using a virus-like particle enrichment procedure, shotgun metagenomic sequencing, and a k-mer-based, reference-dependent taxonomic classification method. Taxonomic composition, diversity, and associations to covariates are presented. We find that the wound virome is highly diverse, with many phages targeting known pathogens, and may influence bacterial community composition and functionality in ways that impact healing outcomes.

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Putative link between Staphylococcus aureus bacteriophage serotype and community association

Cited by 5 publications

References 38 publications

Livestock-Associated Methicillin-Resistant Staphylococcus aureus From Animals and Animal Products in the UK

Livestock-Associated Methicillin-Resistant Staphylococcus aureus From Animals and Animal Products in the UK

Extensive Genetic Diversity Identified among Sporadic Methicillin-Resistant Staphylococcus aureus Isolates Recovered in Irish Hospitals between 2000 and 2012

The chronic wound virome: phage diversity and associations with wounds and healing outcomes

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