Putative novel lineage of West Nile virus in Uranotaenia unguiculata mosquito, Hungary

Kemenesi, Gábor; Dallos, Bianka; Oldal, Miklós; Kutas, Anna; Földes, Fanni; Németh, Viktória; Reiter, Paul L.; Bakonyi, Tamás; Bányai, Krisztiàn; Jakab, Ferenc

doi:10.1007/s13337-014-0234-8

Cited by 21 publications

(17 citation statements)

References 9 publications

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“…To date, nine genetic variants of the virus have been identified. However, the infectivity and neuroinvasive tendencies regarding the lineages have been largely different [2,3]. Among genetic variants, lineage 1 and lineage 2 West Nile virus (ffWNV) strains pose the largest human health risk worldwide [1].…”

Section: Introductionmentioning

confidence: 99%

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Zana

Erdélyi

Nagy

et al. 2020

Viruses

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The West Nile virus is endemic in multiple European countries and responsible for several epidemics throughout the European region. Its evolution into local or even widespread epidemics is driven by multiple factors from genetic diversification of the virus to environmental conditions. The year of 2018 was characterized by an extraordinary increase in human and animal cases in the Central-Eastern European region, including Hungary. In a collaborative effort, we summarized and analyzed the genetic and serologic data of WNV infections from multiple Hungarian public health institutions, universities, and private organizations. We compared human and veterinary serologic data, along with NS5 and NS3 gene sequence data through 2018. Wild birds were excellent indicator species for WNV circulation in each year. Our efforts resulted in documenting the presence of multiple phylogenetic subclades with Balkans and Western-European progenitor sequences of WNV circulating among human and animal populations in Hungary prior to and during the 2018 epidemic. Supported by our sequence and phylogenetic data, the epidemic of 2018 was not caused by recently introduced WNV strains. Unfortunately, Hungary has no country-wide integrated surveillance system which would enable the analysis of related conditions and provide a comprehensive epidemiological picture. The One Health approach, involving multiple institutions and experts, should be implemented in order to fully understand ecological background factors driving the evolution of future epidemics.

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Section: Introductionmentioning

confidence: 99%

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Zana

Erdélyi

Nagy

et al. 2020

Viruses

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“…W est Nile virus (WNV), a widely distributed member of the genus Flavivirus, family Flaviviridae, is responsible for numerous human and animal infections in Europe, Africa, and the Americas. WNV is a single-stranded RNA virus, which is divided into at least eight different genetic lineages (Kemenesi et al 2014, Pachler et al 2014. Lineage 1 and lineage 2 WNV strains are considered the causative agents of both human and animal diseases (Barzon et al 2015).…”

Section: Introductionmentioning

confidence: 99%

West Nile Virus Seroprevalence Among Blood Donors in Hungary

Nagy

Szöllősi

Takács

et al. 2019

Vector-Borne and Zoonotic Diseases

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Background and Objectives: West Nile virus (WNV) is one of the most important viral zoonotic infections in Hungary; however, no transfusion-transmitted WNV infections have been confirmed so far. In 2016, the number of clinical cases of WNV reported was 44, but the seasonal WNV screening of whole-blood donors has not yet been implemented. Our aims were to assess the WNV RNA reactivity and the prevalence of WNV-specific antibodies in the samples of blood donors collected in 2016. Materials and Methods: WNV RNA with Cobas TaqScreen and anti-WNV antibody determination from plasma samples of 2112 donors was performed. Cross-reactivity to tick-borne encephalitis virus was excluded. WNV neutralization test was used for the confirmation of anti-WNV IgG reactive results, and the presence of anti-WNV IgM antibodies was also determined. Results: None of the samples showed WNV RNA reactivity. The total weighted anti-WNV IgG prevalence was 2.34% (95% confidence interval 1.65-3.03), and in addition, three donors were found to be IgM positive. There was a comparable tendency between the data of WNV seroprevalence and cumulative incidence in six out of seven statistical regions in Hungary. Conclusion: Our results show a comparable data with publications that estimated the WNV seroprevalence in some other European endemic areas. As protective measures, both the 30-day deferral of blood donors who spent at least 24 h in WNV-exposed areas and the exclusion of affected Hungarian territories from blood donation are enforced by the Hungarian National Blood Transfusion Service. Our study is the first comprehensive serological survey to obtain actual data about WNV seroprevalence in the Hungarian human population.

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“…WNV strains have been divided into at least eight distinct genetic lineages [ 1 , 2 , 3 ]. The first WNV strain, isolated in Uganda in 1937 [ 4 ], belongs to lineage 2 and this lineage has been confined to sub-Saharan Africa and Madagascar [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and in Vivo Evaluation of Mutations in the NS Region of Lineage 2 West Nile Virus Associated with Neuroinvasiveness in a Mammalian Model

Szentpáli-Gavallér

Lim

Dencső

et al. 2016

Viruses

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West Nile virus (WNV) strains may differ significantly in neuroinvasiveness in vertebrate hosts. In contrast to genetic lineage 1 WNVs, molecular determinants of pathogenic lineage 2 strains have not been experimentally confirmed so far. A full-length infectious clone of a neurovirulent WNV lineage 2 strain (578/10; Central Europe) was generated and amino acid substitutions that have been shown to attenuate lineage 1 WNVs were introduced into the nonstructural proteins (NS1 (P250L), NS2A (A30P), NS3 (P249H) NS4B (P38G, C102S, E249G)). The mouse neuroinvasive phenotype of each mutant virus was examined following intraperitoneal inoculation of C57BL/6 mice. Only the NS1-P250L mutation was associated with a significant attenuation of virulence in mice compared to the wild-type. Multiplication kinetics in cell culture revealed significantly lower infectious virus titres for the NS1 mutant compared to the wild-type, as well as significantly lower amounts of positive and negative stranded RNA.

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Putative novel lineage of West Nile virus in Uranotaenia unguiculata mosquito, Hungary

Cited by 21 publications

References 9 publications

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

West Nile Virus Seroprevalence Among Blood Donors in Hungary

In Vitro and in Vivo Evaluation of Mutations in the NS Region of Lineage 2 West Nile Virus Associated with Neuroinvasiveness in a Mammalian Model

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