Pyocyanine

MacDonald, James C.

doi:10.1007/978-3-642-46054-8_7

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…The pyocyanin assay is based on the absorbance of pyocyanin at 520 nm in acidic solution (15,20). A 5-ml sample of culture grown in PB to maximize pyocyanin production was extracted with 3 ml of chloroform and then reextracted into 1 ml of 0.2 N HCl to give a pink to deep red solution.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications

et al. 1990

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We have found that Pseudomonas aeruginosa has two anthranilate synthase enzymes; they are homologous, and both have conventional ot and p subunits (9b). The two anthranilate synthases have different functions. One participates in tryptophan synthesis; its genes have been designated trpE and trpG (9b). These genes are quite similar to those of Pseudomonas putida (9a). In both pseudomonads, trpG, which encodes the p subunit, is cotranscribed with trpD and trpC in a three-gene operon.The second anthranilate synthase, previously misidentified as the product of a trp gene pair, was obtained on a R-prime plasmid by mating P. aeruginosa PAC174 carrying R68.44 with Escherichia coli W3110 tna AtrpE5 (8). This R-prime plasmid complements only those E. coli auxotrophs blocked in anthranilate synthase, the first enzyme in the tryptophan synthetic pathway. Enzyme assays indicated that both the a and P subunits of anthranilate synthase were produced. The anthranilate synthase-encoding segment of the R-prime plasmid was subcloned into pBR322 (8). DNA sequencing of one of these subclones, pIA14, showed that the genes for the a and P anthranilate synthase subunits were indeed present on the cloned DNA. These genes are adjacent, and their coding sequences overlap by 23 base pairs * Corresponding author. t Present address:

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Section: Methodsmentioning

confidence: 99%

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications

et al. 1990

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“…Labeyrie and Neuzil (1981) showed enhanced growth rate as well as enhanced pyocyanin secretion of P. aeruginosa A237 in amino acids (tyrosine and phenylalanine) supplemented media. The concentration of glycerol and paraffins was found to stimulate the production of pyocyanin and phenazine derivatives in P. aeruginosa (MacDonald 1967). The selective and increased production of PCA and PCN was achieved by Byng et al (1979) using m or p-aminobenzoic acid as selective inhibitor of PYC specifically inhibit of phenazine methylation.…”

Section: Nutritional Factor For Pseudomonadmentioning

confidence: 96%

Fermentative Production of Bacterial Phenazines

Chincholkar

Patil

Sarode

et al. 2013

Microbial Phenazines

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Phenazines, a nitrogen containing heterocyclic antibiotic biosynthesized by a diverse range of bacteria. Owing its enormous importance as (1) electron shuttles to alternate terminal acceptors in bacteria, (2) modify cellular redox states to modify host response, (3) contributing to biofilm formation and cell signaling, as well as (4) biotechnological applications such as environmental sensor, microbial fuel cell, antitumor, and biocontrol activity attracted attention of scientific community to target phenazine as lead molecule. Similarly, emerging application of phenazines insisted high productivity fermentative process. Current chapter focuses on sources of natural phenazines and impact of nutritional as well as environmental dynamics on fermentative production of phenazine in different bacteria.

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Production, characterization and biomedical application of pyocyanin pigment produced by competent Pseudomonas species

Thukkaram,

Muthukrishnan,

2024

Process Biochemistry

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Pyocyanine

Cited by 6 publications

References 19 publications

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications

Fermentative Production of Bacterial Phenazines

Production, characterization and biomedical application of pyocyanin pigment produced by competent Pseudomonas species

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